High Expression of MUC5AC, MUC5B, and Layilin Plays an Essential Role in Prediction in the Development of Plastic Bronchitis Caused by MPP
Plastic bronchitis (PB) is a rare respiratory condition which can result in severe respiratory complications such as respiratory failure and death. Mycoplasma pneumoniae infection is a main etiology cause of plastic bronchitis. However, the pathogenesis of plastic bronchitis complicated by Mycoplasm...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2022-06-01
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| Series: | Frontiers in Microbiology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fmicb.2022.911228/full |
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| author | Yu Ma Yeqi Gu Xinxing Zhang Wenjing Gu Ting Wang Huiming Sun Yinfang Dai Yongdong Yan Yuqing Wang Meijuan Wang Huiquan Sun Chuangli Hao Liping Fan Zhengrong Chen |
| author_facet | Yu Ma Yeqi Gu Xinxing Zhang Wenjing Gu Ting Wang Huiming Sun Yinfang Dai Yongdong Yan Yuqing Wang Meijuan Wang Huiquan Sun Chuangli Hao Liping Fan Zhengrong Chen |
| author_sort | Yu Ma |
| collection | DOAJ |
| description | Plastic bronchitis (PB) is a rare respiratory condition which can result in severe respiratory complications such as respiratory failure and death. Mycoplasma pneumoniae infection is a main etiology cause of plastic bronchitis. However, the pathogenesis of plastic bronchitis complicated by Mycoplasma pneumoniae pneumonia (MPP) has not yet been fully elucidated. Our article aims to explore biomarkers for early prediction of MPP cases complicated with plastic bronchitis. We utilized a protein chip to screen for significantly different proteins among the groups of healthy, general Mycoplasma pneumoniae pneumonia (GMPP) and refractory Mycoplasma pneumoniae pneumonia (RMPP) patients, where layilin exhibited a potent change across biology information technology. Next, we demonstrated the high expression of MUC5AC, MUC5B, and layilin in bronchoalveolar lavage fluid (BALF) of MPP cases complicated with plastic bronchitis. Further study suggested that the level of layilin had a positive correlation with both MUC5AC and MUC5B. A receiver operating characteristic (ROC) analysis was performed to assess the diagnostic values of MUC5AC, MUC5B, and layilin in MPP cases with PB. Data show that the three indicators have similar diagnostic ability for MPP children with plastic bronchitis. Then, we used different concentrations of community-acquired respiratory distress syndrome (CARDS) toxin or lipid-associated membrane proteins (LAMPs) to simulate an in vitro experiment. The in vitro assay revealed that CARDS toxin or LAMPs induced A549 cells to secrete MUC5AC, MUC5B, layilin, and proinflammatory factors. These findings suggest that MUC5AC, MUC5B, and layilin are correlated with MPP. The high expression of MUC5AC, MUC5B, and layilin play an essential role in prediction in the development of plastic bronchitis caused by MPP. The high expression of MUC5AC, MUC5B, and layilin may be relevant to the severity of illness. |
| first_indexed | 2024-12-12T07:47:55Z |
| format | Article |
| id | doaj.art-a2c4800de3f24d36b4e46cec87eb2798 |
| institution | Directory Open Access Journal |
| issn | 1664-302X |
| language | English |
| last_indexed | 2024-12-12T07:47:55Z |
| publishDate | 2022-06-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Microbiology |
| spelling | doaj.art-a2c4800de3f24d36b4e46cec87eb27982022-12-22T00:32:33ZengFrontiers Media S.A.Frontiers in Microbiology1664-302X2022-06-011310.3389/fmicb.2022.911228911228High Expression of MUC5AC, MUC5B, and Layilin Plays an Essential Role in Prediction in the Development of Plastic Bronchitis Caused by MPPYu Ma0Yeqi Gu1Xinxing Zhang2Wenjing Gu3Ting Wang4Huiming Sun5Yinfang Dai6Yongdong Yan7Yuqing Wang8Meijuan Wang9Huiquan Sun10Chuangli Hao11Liping Fan12Zhengrong Chen13Department of Respiratory Medicine, Children's Hospital of Soochow University, Suzhou, ChinaDepartment of Pediatrics, Changzhou Wujin People's Hospital, Changzhou, ChinaDepartment of Respiratory Medicine, Children's Hospital of Soochow University, Suzhou, ChinaDepartment of Respiratory Medicine, Children's Hospital of Soochow University, Suzhou, ChinaDepartment of Respiratory Medicine, Children's Hospital of Soochow University, Suzhou, ChinaDepartment of Respiratory Medicine, Children's Hospital of Soochow University, Suzhou, ChinaDepartment of Respiratory Medicine, Children's Hospital of Soochow University, Suzhou, ChinaDepartment of Respiratory Medicine, Children's Hospital of Soochow University, Suzhou, ChinaDepartment of Respiratory Medicine, Children's Hospital of Soochow University, Suzhou, ChinaDepartment of Respiratory Medicine, Children's Hospital of Soochow University, Suzhou, ChinaDepartment of Respiratory Medicine, Children's Hospital of Soochow University, Suzhou, ChinaDepartment of Respiratory Medicine, Children's Hospital of Soochow University, Suzhou, ChinaDepartment of Respiratory Medicine, Children's Hospital of Soochow University, Suzhou, ChinaDepartment of Respiratory Medicine, Children's Hospital of Soochow University, Suzhou, ChinaPlastic bronchitis (PB) is a rare respiratory condition which can result in severe respiratory complications such as respiratory failure and death. Mycoplasma pneumoniae infection is a main etiology cause of plastic bronchitis. However, the pathogenesis of plastic bronchitis complicated by Mycoplasma pneumoniae pneumonia (MPP) has not yet been fully elucidated. Our article aims to explore biomarkers for early prediction of MPP cases complicated with plastic bronchitis. We utilized a protein chip to screen for significantly different proteins among the groups of healthy, general Mycoplasma pneumoniae pneumonia (GMPP) and refractory Mycoplasma pneumoniae pneumonia (RMPP) patients, where layilin exhibited a potent change across biology information technology. Next, we demonstrated the high expression of MUC5AC, MUC5B, and layilin in bronchoalveolar lavage fluid (BALF) of MPP cases complicated with plastic bronchitis. Further study suggested that the level of layilin had a positive correlation with both MUC5AC and MUC5B. A receiver operating characteristic (ROC) analysis was performed to assess the diagnostic values of MUC5AC, MUC5B, and layilin in MPP cases with PB. Data show that the three indicators have similar diagnostic ability for MPP children with plastic bronchitis. Then, we used different concentrations of community-acquired respiratory distress syndrome (CARDS) toxin or lipid-associated membrane proteins (LAMPs) to simulate an in vitro experiment. The in vitro assay revealed that CARDS toxin or LAMPs induced A549 cells to secrete MUC5AC, MUC5B, layilin, and proinflammatory factors. These findings suggest that MUC5AC, MUC5B, and layilin are correlated with MPP. The high expression of MUC5AC, MUC5B, and layilin play an essential role in prediction in the development of plastic bronchitis caused by MPP. The high expression of MUC5AC, MUC5B, and layilin may be relevant to the severity of illness.https://www.frontiersin.org/articles/10.3389/fmicb.2022.911228/fullMycoplasma pneumoniae pneumonia (MPP)plastic bronchitis (PB)MUC5ACMUC5Blayilin |
| spellingShingle | Yu Ma Yeqi Gu Xinxing Zhang Wenjing Gu Ting Wang Huiming Sun Yinfang Dai Yongdong Yan Yuqing Wang Meijuan Wang Huiquan Sun Chuangli Hao Liping Fan Zhengrong Chen High Expression of MUC5AC, MUC5B, and Layilin Plays an Essential Role in Prediction in the Development of Plastic Bronchitis Caused by MPP Frontiers in Microbiology Mycoplasma pneumoniae pneumonia (MPP) plastic bronchitis (PB) MUC5AC MUC5B layilin |
| title | High Expression of MUC5AC, MUC5B, and Layilin Plays an Essential Role in Prediction in the Development of Plastic Bronchitis Caused by MPP |
| title_full | High Expression of MUC5AC, MUC5B, and Layilin Plays an Essential Role in Prediction in the Development of Plastic Bronchitis Caused by MPP |
| title_fullStr | High Expression of MUC5AC, MUC5B, and Layilin Plays an Essential Role in Prediction in the Development of Plastic Bronchitis Caused by MPP |
| title_full_unstemmed | High Expression of MUC5AC, MUC5B, and Layilin Plays an Essential Role in Prediction in the Development of Plastic Bronchitis Caused by MPP |
| title_short | High Expression of MUC5AC, MUC5B, and Layilin Plays an Essential Role in Prediction in the Development of Plastic Bronchitis Caused by MPP |
| title_sort | high expression of muc5ac muc5b and layilin plays an essential role in prediction in the development of plastic bronchitis caused by mpp |
| topic | Mycoplasma pneumoniae pneumonia (MPP) plastic bronchitis (PB) MUC5AC MUC5B layilin |
| url | https://www.frontiersin.org/articles/10.3389/fmicb.2022.911228/full |
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