In vitro antimicrobial activity of Bixa orellana L. leaves extract against anaerobic bacteria associated to bacterial vaginosis and Lactobacillus spp.
Objective. To describe the in vitro antimicrobial activity of the methanolic extract of Bixa orellana L. leaves against anaerobic bacteria associated to bacterial vaginosis and Lactobacillus spp. Materials and methods. Eight ATCC reference strains; Gardnerella vaginalis, Prevotella bivia, Peptococc...
| Main Authors: | , , , , |
|---|---|
| Format: | Article |
| Language: | Spanish |
| Published: |
Instituto Nacional de Salud
2022-12-01
|
| Series: | Revista Peruana de Medicina Experimental y Salud Pública |
| Subjects: | |
| Online Access: | https://rpmesp.ins.gob.pe/index.php/rpmesp/article/view/11978 |
| _version_ | 1827276395675385856 |
|---|---|
| author | Jenny Marcas Liz Romero Oswaldo Tipiani Steev Loyola Jesús Tamariz |
| author_facet | Jenny Marcas Liz Romero Oswaldo Tipiani Steev Loyola Jesús Tamariz |
| author_sort | Jenny Marcas |
| collection | DOAJ |
| description | Objective. To describe the in vitro antimicrobial activity of the methanolic extract of Bixa orellana L. leaves against anaerobic bacteria associated to bacterial vaginosis and Lactobacillus spp. Materials and methods. Eight ATCC reference strains; Gardnerella vaginalis, Prevotella bivia, Peptococcus niger, Peptostreptococcus anaerobius, Mobiluncus curtisii, Atopobium vaginae, Veillonella parvula, and Lactobacillus crispatus, and twenty-two clinical isolates; eleven Gardnerella vaginalis and eleven Lactobacillus strains, were included in the study. The antimicrobial susceptibility was determined by the agar diffusion method. The minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) were determined by using agar dilution and a modified dilution plating method, respectively. Results. All ATCC reference strains showed
high levels of susceptibility to the extract, except P. vibia, V. parvula and L. crispatus. Interestingly, all G. vaginalis clinical isolates and the G. vaginalis ATTC strain were the most susceptible to the extract, given their low MIC (1.0 – 2.0 mg/mL) and MBC (1.0 – 4.0 mg/mL) values, whereas, the Lactobacillus spp. clinical isolates and the L. crispatus ATCC strain were the least susceptible bacteria given their high MIC (32.0 mg/mL) and MBC (≥ 32.0 mg/mL) values. Conclusions. In vitro experiments suggest that the extract possesses selective antimicrobial properties given its high activity against bacterial vaginosis-associated anaerobic bacteria and low activity against Lactobacillus species. |
| first_indexed | 2024-04-24T06:44:34Z |
| format | Article |
| id | doaj.art-a2c618a84d834d2c8901be13893ca23e |
| institution | Directory Open Access Journal |
| issn | 1726-4634 1726-4642 |
| language | Spanish |
| last_indexed | 2024-04-24T06:44:34Z |
| publishDate | 2022-12-01 |
| publisher | Instituto Nacional de Salud |
| record_format | Article |
| series | Revista Peruana de Medicina Experimental y Salud Pública |
| spelling | doaj.art-a2c618a84d834d2c8901be13893ca23e2024-04-22T19:47:54ZspaInstituto Nacional de SaludRevista Peruana de Medicina Experimental y Salud Pública1726-46341726-46422022-12-014081410.17843/rpmesp.2022.394.119787421In vitro antimicrobial activity of Bixa orellana L. leaves extract against anaerobic bacteria associated to bacterial vaginosis and Lactobacillus spp.Jenny Marcas0https://orcid.org/0000-0003-3312-3050Liz Romero1https://orcid.org/0000-0002-8678-0831Oswaldo Tipiani2https://orcid.org/0000-0002-1325-7249Steev Loyola3https://orcid.org/0000-0001-5455-2423Jesús Tamariz4https://orcid.org/0000-0002-0827-8117Laboratorio de Resistencia Antimicrobiana e Inmunopatología, Universidad Peruana Cayetano Heredia, Lima, PerúLaboratorio de Resistencia Antimicrobiana e Inmunopatología, Universidad Peruana Cayetano Heredia, Lima, PerúHospital Municipal de Surco, Lima, PeruLaboratorio de Resistencia Antimicrobiana e Inmunopatología, Universidad Peruana Cayetano Heredia, Lima, Perú; Facultad de Medicina, Universidad Peruana Cayetano Heredia, Lima, PerúAntimicrobial Resistance and Immunopathology Laboratory, Universidad Peruana Cayetano Heredia, Lima, Peru; Medical school, Universidad Peruana Cayetano Heredia, Lima, PeruObjective. To describe the in vitro antimicrobial activity of the methanolic extract of Bixa orellana L. leaves against anaerobic bacteria associated to bacterial vaginosis and Lactobacillus spp. Materials and methods. Eight ATCC reference strains; Gardnerella vaginalis, Prevotella bivia, Peptococcus niger, Peptostreptococcus anaerobius, Mobiluncus curtisii, Atopobium vaginae, Veillonella parvula, and Lactobacillus crispatus, and twenty-two clinical isolates; eleven Gardnerella vaginalis and eleven Lactobacillus strains, were included in the study. The antimicrobial susceptibility was determined by the agar diffusion method. The minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) were determined by using agar dilution and a modified dilution plating method, respectively. Results. All ATCC reference strains showed high levels of susceptibility to the extract, except P. vibia, V. parvula and L. crispatus. Interestingly, all G. vaginalis clinical isolates and the G. vaginalis ATTC strain were the most susceptible to the extract, given their low MIC (1.0 – 2.0 mg/mL) and MBC (1.0 – 4.0 mg/mL) values, whereas, the Lactobacillus spp. clinical isolates and the L. crispatus ATCC strain were the least susceptible bacteria given their high MIC (32.0 mg/mL) and MBC (≥ 32.0 mg/mL) values. Conclusions. In vitro experiments suggest that the extract possesses selective antimicrobial properties given its high activity against bacterial vaginosis-associated anaerobic bacteria and low activity against Lactobacillus species.https://rpmesp.ins.gob.pe/index.php/rpmesp/article/view/11978bixa orellanavaginosis, bacterialplant extractsin vitro techniquesgardnerella vaginalislactobacillus |
| spellingShingle | Jenny Marcas Liz Romero Oswaldo Tipiani Steev Loyola Jesús Tamariz In vitro antimicrobial activity of Bixa orellana L. leaves extract against anaerobic bacteria associated to bacterial vaginosis and Lactobacillus spp. Revista Peruana de Medicina Experimental y Salud Pública bixa orellana vaginosis, bacterial plant extracts in vitro techniques gardnerella vaginalis lactobacillus |
| title | In vitro antimicrobial activity of Bixa orellana L. leaves extract against anaerobic bacteria associated to bacterial vaginosis and Lactobacillus spp. |
| title_full | In vitro antimicrobial activity of Bixa orellana L. leaves extract against anaerobic bacteria associated to bacterial vaginosis and Lactobacillus spp. |
| title_fullStr | In vitro antimicrobial activity of Bixa orellana L. leaves extract against anaerobic bacteria associated to bacterial vaginosis and Lactobacillus spp. |
| title_full_unstemmed | In vitro antimicrobial activity of Bixa orellana L. leaves extract against anaerobic bacteria associated to bacterial vaginosis and Lactobacillus spp. |
| title_short | In vitro antimicrobial activity of Bixa orellana L. leaves extract against anaerobic bacteria associated to bacterial vaginosis and Lactobacillus spp. |
| title_sort | in vitro antimicrobial activity of bixa orellana l leaves extract against anaerobic bacteria associated to bacterial vaginosis and lactobacillus spp |
| topic | bixa orellana vaginosis, bacterial plant extracts in vitro techniques gardnerella vaginalis lactobacillus |
| url | https://rpmesp.ins.gob.pe/index.php/rpmesp/article/view/11978 |
| work_keys_str_mv | AT jennymarcas invitroantimicrobialactivityofbixaorellanalleavesextractagainstanaerobicbacteriaassociatedtobacterialvaginosisandlactobacillusspp AT lizromero invitroantimicrobialactivityofbixaorellanalleavesextractagainstanaerobicbacteriaassociatedtobacterialvaginosisandlactobacillusspp AT oswaldotipiani invitroantimicrobialactivityofbixaorellanalleavesextractagainstanaerobicbacteriaassociatedtobacterialvaginosisandlactobacillusspp AT steevloyola invitroantimicrobialactivityofbixaorellanalleavesextractagainstanaerobicbacteriaassociatedtobacterialvaginosisandlactobacillusspp AT jesustamariz invitroantimicrobialactivityofbixaorellanalleavesextractagainstanaerobicbacteriaassociatedtobacterialvaginosisandlactobacillusspp |