Bioactive compounds from the parasitic plant Arceuthobium vaginatum inhibit Haemonchus contortus egg hatching
Abstract The aim of this study was to assess the in vitro ovicidal activity of an ethyl acetate extract from Arceuthobium vaginatum (EtOAc-E) and their subfractions (AvR5-AvR14) against Haemonchus contortus using the egg hatching inhibition (EHI) test. The EtOAc-E and subfractions were tested at 0.1...
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| Format: | Article |
| Language: | English |
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Colégio Brasileiro de Parasitologia Veterinaria
2023-12-01
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| Series: | Revista Brasileira de Parasitologia Veterinária |
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| Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-29612024000100301&tlng=en |
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| author | María Mitsi Nalleli Becerril-Gil Julieta Gertrudis Estrada-Flores Manases González-Cortazar Alejandro Zamilpa Ángel Rolando Endara-Agramont Pedro Mendoza-de Gives María Eugenia López-Arellano Agustín Olmedo-Juárez |
| author_facet | María Mitsi Nalleli Becerril-Gil Julieta Gertrudis Estrada-Flores Manases González-Cortazar Alejandro Zamilpa Ángel Rolando Endara-Agramont Pedro Mendoza-de Gives María Eugenia López-Arellano Agustín Olmedo-Juárez |
| author_sort | María Mitsi Nalleli Becerril-Gil |
| collection | DOAJ |
| description | Abstract The aim of this study was to assess the in vitro ovicidal activity of an ethyl acetate extract from Arceuthobium vaginatum (EtOAc-E) and their subfractions (AvR5-AvR14) against Haemonchus contortus using the egg hatching inhibition (EHI) test. The EtOAc-E and subfractions were tested at 0.12-2.00 and at 0.015-2.0 mg/mL, respectively. Distilled water and methanol (2%) were used as negative controls and Thiabendazole (0.10 mg/mL) as a positive control. Treatments with a dependent effect on concentration were subjected to regression analysis to determine the effective concentrations (EC50 and EC90). The major secondary compounds present in the extract and subfractions were identified by high performance liquid chromatography (HPLC). The EtOAc-E and AvR9 exhibited the best ovicidal effect recording 97.5 and 100% of EHI at 0.25 mg/mL, respectively. The EtOAc-E and AvR9 displayed an EC50= 0.12 and 0.08 mg/mL, respectively. The HPLC analysis in the EtOAc-E and bioactive fractions indicated the presence of a polyphenol, glycosylated flavanones, quercetin glucoside, cinnamates, coumarin, cinnamic acid derivative, ferulic acid, coumarate, naringenin, protocatechuic acid and naringin. Results demonstrated that A. vaginatum extract and fraction is able to inhibit the egg hatch process of H. contortus and could be a viable option for the control of small ruminant haemonchosis. |
| first_indexed | 2024-03-08T19:26:42Z |
| format | Article |
| id | doaj.art-a2c7ec53473546bcbfb52fefbeed2e43 |
| institution | Directory Open Access Journal |
| issn | 1984-2961 |
| language | English |
| last_indexed | 2024-03-08T19:26:42Z |
| publishDate | 2023-12-01 |
| publisher | Colégio Brasileiro de Parasitologia Veterinaria |
| record_format | Article |
| series | Revista Brasileira de Parasitologia Veterinária |
| spelling | doaj.art-a2c7ec53473546bcbfb52fefbeed2e432023-12-26T08:28:42ZengColégio Brasileiro de Parasitologia VeterinariaRevista Brasileira de Parasitologia Veterinária1984-29612023-12-0133110.1590/s1984-29612024004Bioactive compounds from the parasitic plant Arceuthobium vaginatum inhibit Haemonchus contortus egg hatchingMaría Mitsi Nalleli Becerril-GilJulieta Gertrudis Estrada-FloresManases González-CortazarAlejandro ZamilpaÁngel Rolando Endara-AgramontPedro Mendoza-de GivesMaría Eugenia López-ArellanoAgustín Olmedo-Juárezhttps://orcid.org/0000-0001-5499-7449Abstract The aim of this study was to assess the in vitro ovicidal activity of an ethyl acetate extract from Arceuthobium vaginatum (EtOAc-E) and their subfractions (AvR5-AvR14) against Haemonchus contortus using the egg hatching inhibition (EHI) test. The EtOAc-E and subfractions were tested at 0.12-2.00 and at 0.015-2.0 mg/mL, respectively. Distilled water and methanol (2%) were used as negative controls and Thiabendazole (0.10 mg/mL) as a positive control. Treatments with a dependent effect on concentration were subjected to regression analysis to determine the effective concentrations (EC50 and EC90). The major secondary compounds present in the extract and subfractions were identified by high performance liquid chromatography (HPLC). The EtOAc-E and AvR9 exhibited the best ovicidal effect recording 97.5 and 100% of EHI at 0.25 mg/mL, respectively. The EtOAc-E and AvR9 displayed an EC50= 0.12 and 0.08 mg/mL, respectively. The HPLC analysis in the EtOAc-E and bioactive fractions indicated the presence of a polyphenol, glycosylated flavanones, quercetin glucoside, cinnamates, coumarin, cinnamic acid derivative, ferulic acid, coumarate, naringenin, protocatechuic acid and naringin. Results demonstrated that A. vaginatum extract and fraction is able to inhibit the egg hatch process of H. contortus and could be a viable option for the control of small ruminant haemonchosis.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-29612024000100301&tlng=enArceuthobiumanthelmintic activitypolyphenolsHaemonchusorganic extract |
| spellingShingle | María Mitsi Nalleli Becerril-Gil Julieta Gertrudis Estrada-Flores Manases González-Cortazar Alejandro Zamilpa Ángel Rolando Endara-Agramont Pedro Mendoza-de Gives María Eugenia López-Arellano Agustín Olmedo-Juárez Bioactive compounds from the parasitic plant Arceuthobium vaginatum inhibit Haemonchus contortus egg hatching Revista Brasileira de Parasitologia Veterinária Arceuthobium anthelmintic activity polyphenols Haemonchus organic extract |
| title | Bioactive compounds from the parasitic plant Arceuthobium vaginatum inhibit Haemonchus contortus egg hatching |
| title_full | Bioactive compounds from the parasitic plant Arceuthobium vaginatum inhibit Haemonchus contortus egg hatching |
| title_fullStr | Bioactive compounds from the parasitic plant Arceuthobium vaginatum inhibit Haemonchus contortus egg hatching |
| title_full_unstemmed | Bioactive compounds from the parasitic plant Arceuthobium vaginatum inhibit Haemonchus contortus egg hatching |
| title_short | Bioactive compounds from the parasitic plant Arceuthobium vaginatum inhibit Haemonchus contortus egg hatching |
| title_sort | bioactive compounds from the parasitic plant arceuthobium vaginatum inhibit haemonchus contortus egg hatching |
| topic | Arceuthobium anthelmintic activity polyphenols Haemonchus organic extract |
| url | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-29612024000100301&tlng=en |
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