Nanopore Sequencing as a Rapidly Deployable Ebola Outbreak Tool

Rapid sequencing of RNA/DNA from pathogen samples obtained during disease outbreaks provides critical scientific and public health information. However, challenges exist for exporting samples to laboratories or establishing conventional sequencers in remote outbreak regions. We successfully used a n...

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Main Authors: Thomas Hoenen, Allison Groseth, Kyle Rosenke, Robert J. Fischer, Andreas Hoenen, Seth D. Judson, Cynthia Martellaro, Darryl Falzarano, Andrea Marzi, R. Burke Squires, Kurt R. Wollenberg, Emmie de Wit, Joseph B. Prescott, David Safronetz, Neeltje van Doremalen, Trenton Bushmaker, Friederike Feldmann, Kristin McNally, Fatorma K. Bolay, Barry Fields, Tara Sealy, Mark Rayfield, Stuart T. Nichol, Kathryn C. Zoon, Moses Massaquoi, Vincent J. Munster, Heinz Feldmann
Format: Article
Language:English
Published: Centers for Disease Control and Prevention 2016-02-01
Series:Emerging Infectious Diseases
Subjects:
Online Access:https://wwwnc.cdc.gov/eid/article/22/2/15-1796_article
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author Thomas Hoenen
Allison Groseth
Kyle Rosenke
Robert J. Fischer
Andreas Hoenen
Seth D. Judson
Cynthia Martellaro
Darryl Falzarano
Andrea Marzi
R. Burke Squires
Kurt R. Wollenberg
Emmie de Wit
Joseph B. Prescott
David Safronetz
Neeltje van Doremalen
Trenton Bushmaker
Friederike Feldmann
Kristin McNally
Fatorma K. Bolay
Barry Fields
Tara Sealy
Mark Rayfield
Stuart T. Nichol
Kathryn C. Zoon
Moses Massaquoi
Vincent J. Munster
Heinz Feldmann
author_facet Thomas Hoenen
Allison Groseth
Kyle Rosenke
Robert J. Fischer
Andreas Hoenen
Seth D. Judson
Cynthia Martellaro
Darryl Falzarano
Andrea Marzi
R. Burke Squires
Kurt R. Wollenberg
Emmie de Wit
Joseph B. Prescott
David Safronetz
Neeltje van Doremalen
Trenton Bushmaker
Friederike Feldmann
Kristin McNally
Fatorma K. Bolay
Barry Fields
Tara Sealy
Mark Rayfield
Stuart T. Nichol
Kathryn C. Zoon
Moses Massaquoi
Vincent J. Munster
Heinz Feldmann
author_sort Thomas Hoenen
collection DOAJ
description Rapid sequencing of RNA/DNA from pathogen samples obtained during disease outbreaks provides critical scientific and public health information. However, challenges exist for exporting samples to laboratories or establishing conventional sequencers in remote outbreak regions. We successfully used a novel, pocket-sized nanopore sequencer at a field diagnostic laboratory in Liberia during the current Ebola virus outbreak.
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spelling doaj.art-a2cc6cfca81b43ebb719175487d2018c2022-12-22T01:30:32ZengCenters for Disease Control and PreventionEmerging Infectious Diseases1080-60401080-60592016-02-0122233133410.3201/eid2202.151796Nanopore Sequencing as a Rapidly Deployable Ebola Outbreak ToolThomas HoenenAllison GrosethKyle RosenkeRobert J. FischerAndreas HoenenSeth D. JudsonCynthia MartellaroDarryl FalzaranoAndrea MarziR. Burke SquiresKurt R. WollenbergEmmie de WitJoseph B. PrescottDavid SafronetzNeeltje van DoremalenTrenton BushmakerFriederike FeldmannKristin McNallyFatorma K. BolayBarry FieldsTara SealyMark RayfieldStuart T. NicholKathryn C. ZoonMoses MassaquoiVincent J. MunsterHeinz FeldmannRapid sequencing of RNA/DNA from pathogen samples obtained during disease outbreaks provides critical scientific and public health information. However, challenges exist for exporting samples to laboratories or establishing conventional sequencers in remote outbreak regions. We successfully used a novel, pocket-sized nanopore sequencer at a field diagnostic laboratory in Liberia during the current Ebola virus outbreak.https://wwwnc.cdc.gov/eid/article/22/2/15-1796_articleEbola hemorrhagic feverEbola virusEbolavirusviruseshigh-throughput nucleotide sequencingnanopore sequencing
spellingShingle Thomas Hoenen
Allison Groseth
Kyle Rosenke
Robert J. Fischer
Andreas Hoenen
Seth D. Judson
Cynthia Martellaro
Darryl Falzarano
Andrea Marzi
R. Burke Squires
Kurt R. Wollenberg
Emmie de Wit
Joseph B. Prescott
David Safronetz
Neeltje van Doremalen
Trenton Bushmaker
Friederike Feldmann
Kristin McNally
Fatorma K. Bolay
Barry Fields
Tara Sealy
Mark Rayfield
Stuart T. Nichol
Kathryn C. Zoon
Moses Massaquoi
Vincent J. Munster
Heinz Feldmann
Nanopore Sequencing as a Rapidly Deployable Ebola Outbreak Tool
Emerging Infectious Diseases
Ebola hemorrhagic fever
Ebola virus
Ebolavirus
viruses
high-throughput nucleotide sequencing
nanopore sequencing
title Nanopore Sequencing as a Rapidly Deployable Ebola Outbreak Tool
title_full Nanopore Sequencing as a Rapidly Deployable Ebola Outbreak Tool
title_fullStr Nanopore Sequencing as a Rapidly Deployable Ebola Outbreak Tool
title_full_unstemmed Nanopore Sequencing as a Rapidly Deployable Ebola Outbreak Tool
title_short Nanopore Sequencing as a Rapidly Deployable Ebola Outbreak Tool
title_sort nanopore sequencing as a rapidly deployable ebola outbreak tool
topic Ebola hemorrhagic fever
Ebola virus
Ebolavirus
viruses
high-throughput nucleotide sequencing
nanopore sequencing
url https://wwwnc.cdc.gov/eid/article/22/2/15-1796_article
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