Phenolics profile, anti-nephrolithiasis, and antioxidant activities of Monodora myristica seed: impact of endogenous proteins and lipids
Abstract The bioactive compounds in a food matrix can exist either in their free form or bound to other endogenous compounds, which may affect their bioactivities. This study investigated the impact of endogenous proteins and lipids on the phenolics profile, anti-nephrolithiasis, and antioxidant act...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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BMC
2023-09-01
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| Series: | Food Production, Processing and Nutrition |
| Subjects: | |
| Online Access: | https://doi.org/10.1186/s43014-023-00167-8 |
| _version_ | 1797689140519632896 |
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| author | Emmanuel Anyachukwu Irondi Tolulope Muktar Aroyehun Arinze Favour Anyiam Milan Kumar Lal |
| author_facet | Emmanuel Anyachukwu Irondi Tolulope Muktar Aroyehun Arinze Favour Anyiam Milan Kumar Lal |
| author_sort | Emmanuel Anyachukwu Irondi |
| collection | DOAJ |
| description | Abstract The bioactive compounds in a food matrix can exist either in their free form or bound to other endogenous compounds, which may affect their bioactivities. This study investigated the impact of endogenous proteins and lipids on the phenolics profile, anti-nephrolithiasis, and antioxidant activities of Monodora myristica (African nutmeg) seed. Endogenous proteins and lipids in M. myristica seed flour were removed by deproteinization and defatting, to obtain the deproteinized, defatted, and deproteinized-defatted flours. The native flour served as a control. Phenolics compounds in the flours were quantified using HPLC-DAD, while nephrolithiasis-associated enzymes (xanthine oxidase and urease) inhibitory and antioxidant activities were determined using a spectrophotometer. Nine phenolic compounds (gallic, chlorogenic, caffeic, p-coumaric, ellagic acids, catechin, rutin, quercetin and luteolin) were detected in the native flour, with quercetin as the most abundant. The concentrations of the phenolic compounds, nephrolithiasis-associated enzymes inhibitory, and antioxidant activities of the flour decreased significantly due to deproteinization and defatting. Overall, the native flour had the highest concentrations of phenolics and the most potent enzymes inhibitory and antioxidant activities, followed by the defatted, deproteinized, and deproteinized-defatted flours. Hence, endogenous proteins and lipids may enhance the phenolics profile, anti-nephrolithiasis and antioxidant activities of M. myristica seed. This could have application in the development of functional food products and nutraceuticals targeting nephrolithiasis. Graphical Abstract |
| first_indexed | 2024-03-12T01:41:40Z |
| format | Article |
| id | doaj.art-a2d2eb9c30f746269f240fbb1f1fcc0a |
| institution | Directory Open Access Journal |
| issn | 2661-8974 |
| language | English |
| last_indexed | 2024-03-12T01:41:40Z |
| publishDate | 2023-09-01 |
| publisher | BMC |
| record_format | Article |
| series | Food Production, Processing and Nutrition |
| spelling | doaj.art-a2d2eb9c30f746269f240fbb1f1fcc0a2023-09-10T11:20:10ZengBMCFood Production, Processing and Nutrition2661-89742023-09-015111010.1186/s43014-023-00167-8Phenolics profile, anti-nephrolithiasis, and antioxidant activities of Monodora myristica seed: impact of endogenous proteins and lipidsEmmanuel Anyachukwu Irondi0Tolulope Muktar Aroyehun1Arinze Favour Anyiam2Milan Kumar Lal3Department of Biochemistry, Kwara State UniversityDepartment of Biochemistry, Kwara State UniversityDepartment of Medical Laboratory Science, Thomas Adewumi UniversityICAR-Central Potato Research InstituteAbstract The bioactive compounds in a food matrix can exist either in their free form or bound to other endogenous compounds, which may affect their bioactivities. This study investigated the impact of endogenous proteins and lipids on the phenolics profile, anti-nephrolithiasis, and antioxidant activities of Monodora myristica (African nutmeg) seed. Endogenous proteins and lipids in M. myristica seed flour were removed by deproteinization and defatting, to obtain the deproteinized, defatted, and deproteinized-defatted flours. The native flour served as a control. Phenolics compounds in the flours were quantified using HPLC-DAD, while nephrolithiasis-associated enzymes (xanthine oxidase and urease) inhibitory and antioxidant activities were determined using a spectrophotometer. Nine phenolic compounds (gallic, chlorogenic, caffeic, p-coumaric, ellagic acids, catechin, rutin, quercetin and luteolin) were detected in the native flour, with quercetin as the most abundant. The concentrations of the phenolic compounds, nephrolithiasis-associated enzymes inhibitory, and antioxidant activities of the flour decreased significantly due to deproteinization and defatting. Overall, the native flour had the highest concentrations of phenolics and the most potent enzymes inhibitory and antioxidant activities, followed by the defatted, deproteinized, and deproteinized-defatted flours. Hence, endogenous proteins and lipids may enhance the phenolics profile, anti-nephrolithiasis and antioxidant activities of M. myristica seed. This could have application in the development of functional food products and nutraceuticals targeting nephrolithiasis. Graphical Abstracthttps://doi.org/10.1186/s43014-023-00167-8AntioxidantsEndogenous proteins and lipidsEnzymes inhibitionMonodora myristicaAnti-nephrolithiasisPhenolic compounds |
| spellingShingle | Emmanuel Anyachukwu Irondi Tolulope Muktar Aroyehun Arinze Favour Anyiam Milan Kumar Lal Phenolics profile, anti-nephrolithiasis, and antioxidant activities of Monodora myristica seed: impact of endogenous proteins and lipids Food Production, Processing and Nutrition Antioxidants Endogenous proteins and lipids Enzymes inhibition Monodora myristica Anti-nephrolithiasis Phenolic compounds |
| title | Phenolics profile, anti-nephrolithiasis, and antioxidant activities of Monodora myristica seed: impact of endogenous proteins and lipids |
| title_full | Phenolics profile, anti-nephrolithiasis, and antioxidant activities of Monodora myristica seed: impact of endogenous proteins and lipids |
| title_fullStr | Phenolics profile, anti-nephrolithiasis, and antioxidant activities of Monodora myristica seed: impact of endogenous proteins and lipids |
| title_full_unstemmed | Phenolics profile, anti-nephrolithiasis, and antioxidant activities of Monodora myristica seed: impact of endogenous proteins and lipids |
| title_short | Phenolics profile, anti-nephrolithiasis, and antioxidant activities of Monodora myristica seed: impact of endogenous proteins and lipids |
| title_sort | phenolics profile anti nephrolithiasis and antioxidant activities of monodora myristica seed impact of endogenous proteins and lipids |
| topic | Antioxidants Endogenous proteins and lipids Enzymes inhibition Monodora myristica Anti-nephrolithiasis Phenolic compounds |
| url | https://doi.org/10.1186/s43014-023-00167-8 |
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