Annexin A1 in plasma from patients with bronchial asthma: its association with lung function
Abstract Background Annexin-A1 (ANXA1) is a glucocorticoid-induced protein with multiple actions in the regulation of inflammatory cell activation. The anti-inflammatory protein ANXA1 and its N-formyl peptide receptor 2 (FPR2) have protective effects on organ fibrosis. However, the exact role of ANX...
| Main Authors: | , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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BMC
2018-01-01
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| Series: | BMC Pulmonary Medicine |
| Subjects: | |
| Online Access: | http://link.springer.com/article/10.1186/s12890-017-0557-5 |
| _version_ | 1818557643488231424 |
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| author | Sun-Hye Lee Pureun-Haneul Lee Byeong-Gon Kim Hyun-Jeong Seo Ae-Rin Baek Jong-Sook Park June-Hyuk Lee Sung-Woo Park Do-Jin Kim Choon-Sik Park An-Soo Jang |
| author_facet | Sun-Hye Lee Pureun-Haneul Lee Byeong-Gon Kim Hyun-Jeong Seo Ae-Rin Baek Jong-Sook Park June-Hyuk Lee Sung-Woo Park Do-Jin Kim Choon-Sik Park An-Soo Jang |
| author_sort | Sun-Hye Lee |
| collection | DOAJ |
| description | Abstract Background Annexin-A1 (ANXA1) is a glucocorticoid-induced protein with multiple actions in the regulation of inflammatory cell activation. The anti-inflammatory protein ANXA1 and its N-formyl peptide receptor 2 (FPR2) have protective effects on organ fibrosis. However, the exact role of ANXA1 in asthma remains to be determined. The aim of this study was to identify the role of ANXA1 in bronchial asthma. Methods In mice sensitized and challenged with ovalbumin (OVA-OVA mice) and mice sensitized with saline and challenged with air (control mice), we investigated the potential links between ANXA1 levels and bronchial asthma using ELISA, immunoblotting, and immunohistochemical staining. Moreover, we also determined ANXA1 levels in blood from 50 asthmatic patients (stable and exacerbated states). Results ANXA1 protein levels in lung tissue and bronchoalveolar lavage fluid were significantly higher in OVA-OVA mice compared with control mice. FPR2 protein levels in lung tissue were significantly higher in OVA-OVA mice compared with control mice. Plasma ANXA1 levels were increased in asthmatic patients compared with healthy controls. Plasma ANXA1 levels were significantly lower in exacerbated patients compared with stable patients with bronchial asthma (p < 0.05). The plasma ANXA1 levels in controlled asthmatic patients were correlated with forced expiratory volume in 1 s (FEV1) (r = − 0.191, p = 0.033) and FEV1/forced vital capacity (FVC) (r = −0.202, p = 0.024). Conclusion These results suggest that ANXA1 may be a potential marker and therapeutic target for asthma. |
| first_indexed | 2024-12-14T00:02:18Z |
| format | Article |
| id | doaj.art-a2d691eb64704b5aafb72c906be60355 |
| institution | Directory Open Access Journal |
| issn | 1471-2466 |
| language | English |
| last_indexed | 2024-12-14T00:02:18Z |
| publishDate | 2018-01-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Pulmonary Medicine |
| spelling | doaj.art-a2d691eb64704b5aafb72c906be603552022-12-21T23:26:16ZengBMCBMC Pulmonary Medicine1471-24662018-01-011811910.1186/s12890-017-0557-5Annexin A1 in plasma from patients with bronchial asthma: its association with lung functionSun-Hye Lee0Pureun-Haneul Lee1Byeong-Gon Kim2Hyun-Jeong Seo3Ae-Rin Baek4Jong-Sook Park5June-Hyuk Lee6Sung-Woo Park7Do-Jin Kim8Choon-Sik Park9An-Soo Jang10Division of Respiratory and Allergy, Department of Internal Medicine, Soonchunhyang University Bucheon HospitalDivision of Respiratory and Allergy, Department of Internal Medicine, Soonchunhyang University Bucheon HospitalDivision of Respiratory and Allergy, Department of Internal Medicine, Soonchunhyang University Bucheon HospitalDivision of Respiratory and Allergy, Department of Internal Medicine, Soonchunhyang University Bucheon HospitalDivision of Respiratory and Allergy, Department of Internal Medicine, Soonchunhyang University Bucheon HospitalDivision of Respiratory and Allergy, Department of Internal Medicine, Soonchunhyang University Bucheon HospitalDivision of Respiratory and Allergy, Department of Internal Medicine, Soonchunhyang University Bucheon HospitalDivision of Respiratory and Allergy, Department of Internal Medicine, Soonchunhyang University Bucheon HospitalDivision of Respiratory and Allergy, Department of Internal Medicine, Soonchunhyang University Bucheon HospitalDivision of Respiratory and Allergy, Department of Internal Medicine, Soonchunhyang University Bucheon HospitalDivision of Respiratory and Allergy, Department of Internal Medicine, Soonchunhyang University Bucheon HospitalAbstract Background Annexin-A1 (ANXA1) is a glucocorticoid-induced protein with multiple actions in the regulation of inflammatory cell activation. The anti-inflammatory protein ANXA1 and its N-formyl peptide receptor 2 (FPR2) have protective effects on organ fibrosis. However, the exact role of ANXA1 in asthma remains to be determined. The aim of this study was to identify the role of ANXA1 in bronchial asthma. Methods In mice sensitized and challenged with ovalbumin (OVA-OVA mice) and mice sensitized with saline and challenged with air (control mice), we investigated the potential links between ANXA1 levels and bronchial asthma using ELISA, immunoblotting, and immunohistochemical staining. Moreover, we also determined ANXA1 levels in blood from 50 asthmatic patients (stable and exacerbated states). Results ANXA1 protein levels in lung tissue and bronchoalveolar lavage fluid were significantly higher in OVA-OVA mice compared with control mice. FPR2 protein levels in lung tissue were significantly higher in OVA-OVA mice compared with control mice. Plasma ANXA1 levels were increased in asthmatic patients compared with healthy controls. Plasma ANXA1 levels were significantly lower in exacerbated patients compared with stable patients with bronchial asthma (p < 0.05). The plasma ANXA1 levels in controlled asthmatic patients were correlated with forced expiratory volume in 1 s (FEV1) (r = − 0.191, p = 0.033) and FEV1/forced vital capacity (FVC) (r = −0.202, p = 0.024). Conclusion These results suggest that ANXA1 may be a potential marker and therapeutic target for asthma.http://link.springer.com/article/10.1186/s12890-017-0557-5Airway epithelialAnnexin A1AsthmaBloodFPR2Ovalbumin |
| spellingShingle | Sun-Hye Lee Pureun-Haneul Lee Byeong-Gon Kim Hyun-Jeong Seo Ae-Rin Baek Jong-Sook Park June-Hyuk Lee Sung-Woo Park Do-Jin Kim Choon-Sik Park An-Soo Jang Annexin A1 in plasma from patients with bronchial asthma: its association with lung function BMC Pulmonary Medicine Airway epithelial Annexin A1 Asthma Blood FPR2 Ovalbumin |
| title | Annexin A1 in plasma from patients with bronchial asthma: its association with lung function |
| title_full | Annexin A1 in plasma from patients with bronchial asthma: its association with lung function |
| title_fullStr | Annexin A1 in plasma from patients with bronchial asthma: its association with lung function |
| title_full_unstemmed | Annexin A1 in plasma from patients with bronchial asthma: its association with lung function |
| title_short | Annexin A1 in plasma from patients with bronchial asthma: its association with lung function |
| title_sort | annexin a1 in plasma from patients with bronchial asthma its association with lung function |
| topic | Airway epithelial Annexin A1 Asthma Blood FPR2 Ovalbumin |
| url | http://link.springer.com/article/10.1186/s12890-017-0557-5 |
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