Molecular Diagnostic of Ochratoxin A With Specific Aptamers in Corn and Groundnut via Fabrication of a Microfluidic Device
Ochratoxin A (OTA) is one of the predominant mycotoxins that contaminate a wide range of food commodities. In the present study, a 36-mer aptamer was used as a molecular recognition element coupled with gold nanoparticles (AuNPs) for colorimetric detection of OTA in a microfluidic paper-based analyt...
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Frontiers Media S.A.
2022-03-01
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| Series: | Frontiers in Nutrition |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fnut.2022.851787/full |
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| author | Deepshikha Shahdeo Azmat Ali Khan Amer M. Alanazi Vivek K. Bajpai Shruti Shukla Sonu Gandhi |
| author_facet | Deepshikha Shahdeo Azmat Ali Khan Amer M. Alanazi Vivek K. Bajpai Shruti Shukla Sonu Gandhi |
| author_sort | Deepshikha Shahdeo |
| collection | DOAJ |
| description | Ochratoxin A (OTA) is one of the predominant mycotoxins that contaminate a wide range of food commodities. In the present study, a 36-mer aptamer was used as a molecular recognition element coupled with gold nanoparticles (AuNPs) for colorimetric detection of OTA in a microfluidic paper-based analytical device (μPADs). The μPADs consisted of three zones: control, detection, and sample, interconnected by channels. UV-vis spectroscopy (UV-vis), Dynamic Light Scattering (DLS), and Transmission Electron Microscopy (TEM) were used for characterization of AuNPs and AuNPs/Aptamer. According to the colorimetric assay, limit of detection (LOD) was found to be 242, 545.45, and 95.69 ng/mL in water, corn, and groundnut, respectively. The HPLC detection method achieved acceptable coefficient in standard curves (r2 = 0.9995), improved detection range, and recovery rates in spiked corn and groundnut samples as 43.61 ± 2.18% to 87.10 ± 1.82% and 42.01 ± 1.31% to 86.03 ± 2.64% after multiple sample extractions and cleanup steps. However, the developed μPADs analytical device had the potent ability to rapidly detect OTA without any extraction pre-requirement, derivatization, and cleanup steps, thus illustrating its feasibility in the animal health sector, agricultural, and food industries. |
| first_indexed | 2024-12-13T08:12:48Z |
| format | Article |
| id | doaj.art-a2d789a90aed4f9ba5bd9bfd47825e82 |
| institution | Directory Open Access Journal |
| issn | 2296-861X |
| language | English |
| last_indexed | 2024-12-13T08:12:48Z |
| publishDate | 2022-03-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Nutrition |
| spelling | doaj.art-a2d789a90aed4f9ba5bd9bfd47825e822022-12-21T23:54:11ZengFrontiers Media S.A.Frontiers in Nutrition2296-861X2022-03-01910.3389/fnut.2022.851787851787Molecular Diagnostic of Ochratoxin A With Specific Aptamers in Corn and Groundnut via Fabrication of a Microfluidic DeviceDeepshikha Shahdeo0Azmat Ali Khan1Amer M. Alanazi2Vivek K. Bajpai3Shruti Shukla4Sonu Gandhi5DBT-National Institute of Animal Biotechnology (NIAB), Hyderabad, IndiaPharmaceutical Biotechnology Laboratory, Department of Pharmaceutical Chemistry, College of Pharmacy, King Saud University, Riyadh, Saudi ArabiaPharmaceutical Biotechnology Laboratory, Department of Pharmaceutical Chemistry, College of Pharmacy, King Saud University, Riyadh, Saudi ArabiaDepartment of Energy and Materials Engineering, Dongguk University, Seoul, South KoreaTERI-Deakin Nanobiotechnology Centre, Sustainable Agricultural Division, The Energy and Resources Institute, TERI Gram, Gurugram, IndiaDBT-National Institute of Animal Biotechnology (NIAB), Hyderabad, IndiaOchratoxin A (OTA) is one of the predominant mycotoxins that contaminate a wide range of food commodities. In the present study, a 36-mer aptamer was used as a molecular recognition element coupled with gold nanoparticles (AuNPs) for colorimetric detection of OTA in a microfluidic paper-based analytical device (μPADs). The μPADs consisted of three zones: control, detection, and sample, interconnected by channels. UV-vis spectroscopy (UV-vis), Dynamic Light Scattering (DLS), and Transmission Electron Microscopy (TEM) were used for characterization of AuNPs and AuNPs/Aptamer. According to the colorimetric assay, limit of detection (LOD) was found to be 242, 545.45, and 95.69 ng/mL in water, corn, and groundnut, respectively. The HPLC detection method achieved acceptable coefficient in standard curves (r2 = 0.9995), improved detection range, and recovery rates in spiked corn and groundnut samples as 43.61 ± 2.18% to 87.10 ± 1.82% and 42.01 ± 1.31% to 86.03 ± 2.64% after multiple sample extractions and cleanup steps. However, the developed μPADs analytical device had the potent ability to rapidly detect OTA without any extraction pre-requirement, derivatization, and cleanup steps, thus illustrating its feasibility in the animal health sector, agricultural, and food industries.https://www.frontiersin.org/articles/10.3389/fnut.2022.851787/fullgold nanoparticlescolorimetric assayOchratoxin Amicrofluidicaptasensor |
| spellingShingle | Deepshikha Shahdeo Azmat Ali Khan Amer M. Alanazi Vivek K. Bajpai Shruti Shukla Sonu Gandhi Molecular Diagnostic of Ochratoxin A With Specific Aptamers in Corn and Groundnut via Fabrication of a Microfluidic Device Frontiers in Nutrition gold nanoparticles colorimetric assay Ochratoxin A microfluidic aptasensor |
| title | Molecular Diagnostic of Ochratoxin A With Specific Aptamers in Corn and Groundnut via Fabrication of a Microfluidic Device |
| title_full | Molecular Diagnostic of Ochratoxin A With Specific Aptamers in Corn and Groundnut via Fabrication of a Microfluidic Device |
| title_fullStr | Molecular Diagnostic of Ochratoxin A With Specific Aptamers in Corn and Groundnut via Fabrication of a Microfluidic Device |
| title_full_unstemmed | Molecular Diagnostic of Ochratoxin A With Specific Aptamers in Corn and Groundnut via Fabrication of a Microfluidic Device |
| title_short | Molecular Diagnostic of Ochratoxin A With Specific Aptamers in Corn and Groundnut via Fabrication of a Microfluidic Device |
| title_sort | molecular diagnostic of ochratoxin a with specific aptamers in corn and groundnut via fabrication of a microfluidic device |
| topic | gold nanoparticles colorimetric assay Ochratoxin A microfluidic aptasensor |
| url | https://www.frontiersin.org/articles/10.3389/fnut.2022.851787/full |
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