Effect of Chloroform Application on Roughness and Wettability of the Root Canal Walls in Endodontic Retreatment

Statement of the Problem: The success of root canal therapy depends on root canal irrigation, disinfection, and sealing of root canal. Wettability and roughness of root dentine surface are important factors in root canal disinfection and sealing.Purpose: This study aimed to assess the effect of chlo...

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Main Authors: Davoud Jamshidi, Mohammadreza Tahriri, Heydar Mosleh, Mohammad Madadpour, Soolmaz Heidari, Mahmood Alipour Heydari, Mohammad Javad Kharazi Fard
Format: Article
Language:English
Published: Shiraz University of Medical Sciences 2022-09-01
Series:Journal of Dentistry
Subjects:
Online Access:https://dentjods.sums.ac.ir/article_48396_d561f61fb53c038ddf1022f212bbef49.pdf
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author Davoud Jamshidi
Mohammadreza Tahriri
Heydar Mosleh
Mohammad Madadpour
Soolmaz Heidari
Mahmood Alipour Heydari
Mohammad Javad Kharazi Fard
author_facet Davoud Jamshidi
Mohammadreza Tahriri
Heydar Mosleh
Mohammad Madadpour
Soolmaz Heidari
Mahmood Alipour Heydari
Mohammad Javad Kharazi Fard
author_sort Davoud Jamshidi
collection DOAJ
description Statement of the Problem: The success of root canal therapy depends on root canal irrigation, disinfection, and sealing of root canal. Wettability and roughness of root dentine surface are important factors in root canal disinfection and sealing.Purpose: This study aimed to assess the effect of chloroform application on roughness and wettability of the root canal walls in endodontic retreatment.Materials and Method: This in vitro experimental study evaluated 70 sound extracted human anterior teeth. The specimens were then randomly assigned to 7 groups as follows: Chloroform (group 1), 1g gutta percha+chloroform (group 2), 2g gutta percha+ chloroform (group 3), 1g gutta percha+ 1g sealer+ chloroform (group 4), 2g gutta perch+ 2g sealer+chloroform (group 5), 1g sealer + chloroform (group 6) and 2g sealer + chloroform (group 7). One drop of distilled water was placed on each tooth to measure the contact angle and wettability. Photographs were obtained of an area measuring 50×50 µm2 in three directions under an atomic force microscope to measure the roughness. The tooth blocks were exposed to the abovementioned mixtures for 10 min, and then rinsed with saline. The roughness and wettability of each sample were measured before and after treatment. Data was analyzed using one-way ANOVA and Tukey’s test.Results: The roughness of all groups significantly decreased following treatment, except for groups 1 and 4. The contact angle increased in all groups after treatment (except for the chloroform group), which indicated decreased wettability. The roughness and the contact angle have shown no correlation.Conclusion: Application of chloroform for removal of gutta-percha and sealer in endodontic retreatment decreases the roughness and wettability of dentine.
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spelling doaj.art-a2d87fdfc7c9493fb8917de943483b682022-12-22T03:17:23ZengShiraz University of Medical SciencesJournal of Dentistry2345-64852345-64182022-09-0123327227710.30476/dentjods.2021.88414.132948396Effect of Chloroform Application on Roughness and Wettability of the Root Canal Walls in Endodontic RetreatmentDavoud Jamshidi0Mohammadreza Tahriri1Heydar Mosleh2Mohammad Madadpour3Soolmaz Heidari4Mahmood Alipour Heydari5Mohammad Javad Kharazi Fard6Dept. of Endodontics, School of Dentistry, Qazvin University of Medical Sciences, Qazvin, Iran.Postdoctoral Fellow Researcher, Marquette University School of Dentistry, Milwaukee, WI 53233, USA.General Dentist, Student Research Committee, Qazvin University of Medical Sciences, Qazvin, Iran.General Dentist, Student Research Committee, Qazvin University of Medical Sciences, Qazvin, Iran.Dept. of Operative Dentistry, Dental Caries Prevention Research Center, Qazvin University of Medical Sciences, Qazvin, Iran.Dept. of Biostatistics, Qazvin University of Medical Sciences, Qazvin, Iran.Dental Research Center, Tehran University of Medical Sciences, Tehran, Iran.Statement of the Problem: The success of root canal therapy depends on root canal irrigation, disinfection, and sealing of root canal. Wettability and roughness of root dentine surface are important factors in root canal disinfection and sealing.Purpose: This study aimed to assess the effect of chloroform application on roughness and wettability of the root canal walls in endodontic retreatment.Materials and Method: This in vitro experimental study evaluated 70 sound extracted human anterior teeth. The specimens were then randomly assigned to 7 groups as follows: Chloroform (group 1), 1g gutta percha+chloroform (group 2), 2g gutta percha+ chloroform (group 3), 1g gutta percha+ 1g sealer+ chloroform (group 4), 2g gutta perch+ 2g sealer+chloroform (group 5), 1g sealer + chloroform (group 6) and 2g sealer + chloroform (group 7). One drop of distilled water was placed on each tooth to measure the contact angle and wettability. Photographs were obtained of an area measuring 50×50 µm2 in three directions under an atomic force microscope to measure the roughness. The tooth blocks were exposed to the abovementioned mixtures for 10 min, and then rinsed with saline. The roughness and wettability of each sample were measured before and after treatment. Data was analyzed using one-way ANOVA and Tukey’s test.Results: The roughness of all groups significantly decreased following treatment, except for groups 1 and 4. The contact angle increased in all groups after treatment (except for the chloroform group), which indicated decreased wettability. The roughness and the contact angle have shown no correlation.Conclusion: Application of chloroform for removal of gutta-percha and sealer in endodontic retreatment decreases the roughness and wettability of dentine.https://dentjods.sums.ac.ir/article_48396_d561f61fb53c038ddf1022f212bbef49.pdfsealerchloroformgutta perchadentincontact angle
spellingShingle Davoud Jamshidi
Mohammadreza Tahriri
Heydar Mosleh
Mohammad Madadpour
Soolmaz Heidari
Mahmood Alipour Heydari
Mohammad Javad Kharazi Fard
Effect of Chloroform Application on Roughness and Wettability of the Root Canal Walls in Endodontic Retreatment
Journal of Dentistry
sealer
chloroform
gutta percha
dentin
contact angle
title Effect of Chloroform Application on Roughness and Wettability of the Root Canal Walls in Endodontic Retreatment
title_full Effect of Chloroform Application on Roughness and Wettability of the Root Canal Walls in Endodontic Retreatment
title_fullStr Effect of Chloroform Application on Roughness and Wettability of the Root Canal Walls in Endodontic Retreatment
title_full_unstemmed Effect of Chloroform Application on Roughness and Wettability of the Root Canal Walls in Endodontic Retreatment
title_short Effect of Chloroform Application on Roughness and Wettability of the Root Canal Walls in Endodontic Retreatment
title_sort effect of chloroform application on roughness and wettability of the root canal walls in endodontic retreatment
topic sealer
chloroform
gutta percha
dentin
contact angle
url https://dentjods.sums.ac.ir/article_48396_d561f61fb53c038ddf1022f212bbef49.pdf
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