Pysanka-Inspired Electrode Modification with Aptamer Encapsulation in ZIF-8 for Urine Creatinine Electrochemical Biosensing
Drawing inspiration from the several thousand beautiful Pysanky egg art of Ukraine, we have developed a novel material, Aptamer–Gold Nanoparticles (AuNPs)@ZIF-8, that can be used for building sensitive and highly stable POC biosensors for longitudinal health mapping. Here, we demonstrate a sensitive...
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| Format: | Article |
| Language: | English |
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MDPI AG
2023-11-01
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| Series: | Chemosensors |
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| Online Access: | https://www.mdpi.com/2227-9040/11/11/557 |
| _version_ | 1797459761135878144 |
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| author | Antra Ganguly Anirban Paul Shalini Prasad |
| author_facet | Antra Ganguly Anirban Paul Shalini Prasad |
| author_sort | Antra Ganguly |
| collection | DOAJ |
| description | Drawing inspiration from the several thousand beautiful Pysanky egg art of Ukraine, we have developed a novel material, Aptamer–Gold Nanoparticles (AuNPs)@ZIF-8, that can be used for building sensitive and highly stable POC biosensors for longitudinal health mapping. Here, we demonstrate a sensitive and specific novel electrochemical biosensor, made of a novel synthesized in situ encapsulated aptamer-AuNPs@ZIF-8 composite, for monitoring levels of creatinine (0.1–1000 μg/mL). In this work, we have reported the synthetic protocol for the first-of-a-kind in situ encapsulation of aptamer and AuNPs together in a ZIF-8 matrix, and explored the characteristic properties of this novel material composite using standard analytical techniques and its application for biosensor application. The as-synthesized material, duly characterized using various physicochemical analytical methods, portrays the characteristics of the unique encapsulation strategy to develop the first-of-a-kind aptamer and AuNP encapsulation. Non-faradaic Electrochemical Impedance Spectroscopy (EIS) and Chronoamperometry were used to characterize the interfacial electrochemical properties. The biosensor performance was first validated using artificial urine in a controlled buffer medium. The stability and robustness were tested using a real human urine medium without filtration or sample treatment. Being versatile, this Ukrainian-art-inspired biosensor can potentially move the needle towards developing the next generation of sample-in-result-out robust POC diagnostics. |
| first_indexed | 2024-03-09T16:55:58Z |
| format | Article |
| id | doaj.art-a2de86682f0d494991261e707d80a3c0 |
| institution | Directory Open Access Journal |
| issn | 2227-9040 |
| language | English |
| last_indexed | 2024-03-09T16:55:58Z |
| publishDate | 2023-11-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Chemosensors |
| spelling | doaj.art-a2de86682f0d494991261e707d80a3c02023-11-24T14:35:35ZengMDPI AGChemosensors2227-90402023-11-01111155710.3390/chemosensors11110557Pysanka-Inspired Electrode Modification with Aptamer Encapsulation in ZIF-8 for Urine Creatinine Electrochemical BiosensingAntra Ganguly0Anirban Paul1Shalini Prasad2Department of Bioengineering, University of Texas at Dallas, Richardson, TX 75080, USADepartment of Bioengineering, University of Texas at Dallas, Richardson, TX 75080, USADepartment of Bioengineering, University of Texas at Dallas, Richardson, TX 75080, USADrawing inspiration from the several thousand beautiful Pysanky egg art of Ukraine, we have developed a novel material, Aptamer–Gold Nanoparticles (AuNPs)@ZIF-8, that can be used for building sensitive and highly stable POC biosensors for longitudinal health mapping. Here, we demonstrate a sensitive and specific novel electrochemical biosensor, made of a novel synthesized in situ encapsulated aptamer-AuNPs@ZIF-8 composite, for monitoring levels of creatinine (0.1–1000 μg/mL). In this work, we have reported the synthetic protocol for the first-of-a-kind in situ encapsulation of aptamer and AuNPs together in a ZIF-8 matrix, and explored the characteristic properties of this novel material composite using standard analytical techniques and its application for biosensor application. The as-synthesized material, duly characterized using various physicochemical analytical methods, portrays the characteristics of the unique encapsulation strategy to develop the first-of-a-kind aptamer and AuNP encapsulation. Non-faradaic Electrochemical Impedance Spectroscopy (EIS) and Chronoamperometry were used to characterize the interfacial electrochemical properties. The biosensor performance was first validated using artificial urine in a controlled buffer medium. The stability and robustness were tested using a real human urine medium without filtration or sample treatment. Being versatile, this Ukrainian-art-inspired biosensor can potentially move the needle towards developing the next generation of sample-in-result-out robust POC diagnostics.https://www.mdpi.com/2227-9040/11/11/557encapsulated ZIF-8metal–organic framework biosensorart-inspired chemosensorPysanky biosensor point-of-care (POC) technologyelectrochemical creatinine POCcreatinine aptamer chemosensor |
| spellingShingle | Antra Ganguly Anirban Paul Shalini Prasad Pysanka-Inspired Electrode Modification with Aptamer Encapsulation in ZIF-8 for Urine Creatinine Electrochemical Biosensing Chemosensors encapsulated ZIF-8 metal–organic framework biosensor art-inspired chemosensor Pysanky biosensor point-of-care (POC) technology electrochemical creatinine POC creatinine aptamer chemosensor |
| title | Pysanka-Inspired Electrode Modification with Aptamer Encapsulation in ZIF-8 for Urine Creatinine Electrochemical Biosensing |
| title_full | Pysanka-Inspired Electrode Modification with Aptamer Encapsulation in ZIF-8 for Urine Creatinine Electrochemical Biosensing |
| title_fullStr | Pysanka-Inspired Electrode Modification with Aptamer Encapsulation in ZIF-8 for Urine Creatinine Electrochemical Biosensing |
| title_full_unstemmed | Pysanka-Inspired Electrode Modification with Aptamer Encapsulation in ZIF-8 for Urine Creatinine Electrochemical Biosensing |
| title_short | Pysanka-Inspired Electrode Modification with Aptamer Encapsulation in ZIF-8 for Urine Creatinine Electrochemical Biosensing |
| title_sort | pysanka inspired electrode modification with aptamer encapsulation in zif 8 for urine creatinine electrochemical biosensing |
| topic | encapsulated ZIF-8 metal–organic framework biosensor art-inspired chemosensor Pysanky biosensor point-of-care (POC) technology electrochemical creatinine POC creatinine aptamer chemosensor |
| url | https://www.mdpi.com/2227-9040/11/11/557 |
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