Raman Spectroscopy Characterization of Multi-Functionalized Liposomes as Drug-Delivery Systems for Neurological Disorders
The characterization of nanoparticle-based drug-delivery systems represents a crucial step in achieving a comprehensive overview of their physical, chemical, and biological features and evaluating their efficacy and safety in biological systems. We propose Raman Spectroscopy (RS) for the characteriz...
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| Format: | Article |
| Language: | English |
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MDPI AG
2023-02-01
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| Series: | Nanomaterials |
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| Online Access: | https://www.mdpi.com/2079-4991/13/4/699 |
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| author | Francesca Rodà Silvia Picciolini Valentina Mangolini Alice Gualerzi Pierfausto Seneci Antonio Renda Silvia Sesana Francesca Re Marzia Bedoni |
| author_facet | Francesca Rodà Silvia Picciolini Valentina Mangolini Alice Gualerzi Pierfausto Seneci Antonio Renda Silvia Sesana Francesca Re Marzia Bedoni |
| author_sort | Francesca Rodà |
| collection | DOAJ |
| description | The characterization of nanoparticle-based drug-delivery systems represents a crucial step in achieving a comprehensive overview of their physical, chemical, and biological features and evaluating their efficacy and safety in biological systems. We propose Raman Spectroscopy (RS) for the characterization of liposomes (LPs) to be tested for the control of neuroinflammation and microglial dysfunctions in Glioblastoma multiforme and Alzheimer’s disease. Drug-loaded LPs were functionalized to cross the blood–brain barrier and to guarantee localized and controlled drug release. The Raman spectra of each LP component were used to evaluate their contribution in the LP Raman fingerprint. Raman data analysis made it possible to statistically discriminate LPs with different functionalization patterns, showing that each molecular component has an influence in the Raman spectrum of the final LP formulation. Moreover, CLS analysis on Raman data revealed a good level of synthetic reproducibility of the formulations and confirmed their stability within one month from their synthesis, demonstrating the ability of the technique to evaluate the efficacy of LP synthesis using small amount of sample. RS represents a valuable tool for a fast, sensitive and label free biochemical characterization of LPs that could be used for quality control of nanoparticle-based therapeutics. |
| first_indexed | 2024-03-11T08:20:06Z |
| format | Article |
| id | doaj.art-a2e1c8c916ee4ec4be31e2e3ac630e5b |
| institution | Directory Open Access Journal |
| issn | 2079-4991 |
| language | English |
| last_indexed | 2024-03-11T08:20:06Z |
| publishDate | 2023-02-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Nanomaterials |
| spelling | doaj.art-a2e1c8c916ee4ec4be31e2e3ac630e5b2023-11-16T22:27:43ZengMDPI AGNanomaterials2079-49912023-02-0113469910.3390/nano13040699Raman Spectroscopy Characterization of Multi-Functionalized Liposomes as Drug-Delivery Systems for Neurological DisordersFrancesca Rodà0Silvia Picciolini1Valentina Mangolini2Alice Gualerzi3Pierfausto Seneci4Antonio Renda5Silvia Sesana6Francesca Re7Marzia Bedoni8IRCCS Fondazione Don Carlo Gnocchi ONLUS, 20148 Milan, ItalyIRCCS Fondazione Don Carlo Gnocchi ONLUS, 20148 Milan, ItalyIRCCS Fondazione Don Carlo Gnocchi ONLUS, 20148 Milan, ItalyIRCCS Fondazione Don Carlo Gnocchi ONLUS, 20148 Milan, ItalyChemistry Department, Università Degli Studi di Milano, 20133 Milan, ItalySchool of Medicine and Surgery, University of Milano-Bicocca, 20854 Vedano al Lambro, ItalySchool of Medicine and Surgery, University of Milano-Bicocca, 20854 Vedano al Lambro, ItalySchool of Medicine and Surgery, University of Milano-Bicocca, 20854 Vedano al Lambro, ItalyIRCCS Fondazione Don Carlo Gnocchi ONLUS, 20148 Milan, ItalyThe characterization of nanoparticle-based drug-delivery systems represents a crucial step in achieving a comprehensive overview of their physical, chemical, and biological features and evaluating their efficacy and safety in biological systems. We propose Raman Spectroscopy (RS) for the characterization of liposomes (LPs) to be tested for the control of neuroinflammation and microglial dysfunctions in Glioblastoma multiforme and Alzheimer’s disease. Drug-loaded LPs were functionalized to cross the blood–brain barrier and to guarantee localized and controlled drug release. The Raman spectra of each LP component were used to evaluate their contribution in the LP Raman fingerprint. Raman data analysis made it possible to statistically discriminate LPs with different functionalization patterns, showing that each molecular component has an influence in the Raman spectrum of the final LP formulation. Moreover, CLS analysis on Raman data revealed a good level of synthetic reproducibility of the formulations and confirmed their stability within one month from their synthesis, demonstrating the ability of the technique to evaluate the efficacy of LP synthesis using small amount of sample. RS represents a valuable tool for a fast, sensitive and label free biochemical characterization of LPs that could be used for quality control of nanoparticle-based therapeutics.https://www.mdpi.com/2079-4991/13/4/699liposomeRaman spectroscopydrug deliveryneurological disorderstherapeuticsmultivariate analysis |
| spellingShingle | Francesca Rodà Silvia Picciolini Valentina Mangolini Alice Gualerzi Pierfausto Seneci Antonio Renda Silvia Sesana Francesca Re Marzia Bedoni Raman Spectroscopy Characterization of Multi-Functionalized Liposomes as Drug-Delivery Systems for Neurological Disorders Nanomaterials liposome Raman spectroscopy drug delivery neurological disorders therapeutics multivariate analysis |
| title | Raman Spectroscopy Characterization of Multi-Functionalized Liposomes as Drug-Delivery Systems for Neurological Disorders |
| title_full | Raman Spectroscopy Characterization of Multi-Functionalized Liposomes as Drug-Delivery Systems for Neurological Disorders |
| title_fullStr | Raman Spectroscopy Characterization of Multi-Functionalized Liposomes as Drug-Delivery Systems for Neurological Disorders |
| title_full_unstemmed | Raman Spectroscopy Characterization of Multi-Functionalized Liposomes as Drug-Delivery Systems for Neurological Disorders |
| title_short | Raman Spectroscopy Characterization of Multi-Functionalized Liposomes as Drug-Delivery Systems for Neurological Disorders |
| title_sort | raman spectroscopy characterization of multi functionalized liposomes as drug delivery systems for neurological disorders |
| topic | liposome Raman spectroscopy drug delivery neurological disorders therapeutics multivariate analysis |
| url | https://www.mdpi.com/2079-4991/13/4/699 |
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