Identification and Characterization of Jasmonic Acid Biosynthetic Genes in <i>Salvia miltiorrhiza</i> Bunge
Jasmonic acid (JA) is a vital plant hormone that performs a variety of critical functions for plants. <i>Salvia miltiorrhiza</i> Bunge (<i>S. miltiorrhiza</i>), also known as Danshen, is a renowned traditional Chinese medicinal herb. However, no thorough and systematic analys...
| Main Authors: | , , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2022-08-01
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| Series: | International Journal of Molecular Sciences |
| Subjects: | |
| Online Access: | https://www.mdpi.com/1422-0067/23/16/9384 |
| Summary: | Jasmonic acid (JA) is a vital plant hormone that performs a variety of critical functions for plants. <i>Salvia miltiorrhiza</i> Bunge (<i>S. miltiorrhiza</i>), also known as Danshen, is a renowned traditional Chinese medicinal herb. However, no thorough and systematic analysis of JA biosynthesis genes in <i>S. miltiorrhiza</i> exists. Through genome-wide prediction and molecular cloning, 23 candidate genes related to JA biosynthesis were identified in <i>S. miltiorrhiza</i>. These genes belong to four families that encode lipoxygenase (LOX), allene oxide synthase (AOS), allene oxide cyclase (AOC), and 12-OPDA reductase3 (OPR3). It was discovered that the candidate genes for JA synthesis of <i>S. miltiorrhiza</i> were distinct and conserved, in contrast to related genes in other plants, by evaluating their genetic structures, protein characteristics, and phylogenetic trees. These genes displayed tissue-specific expression patterns concerning to methyl jasmonate (MeJA) and wound tests. Overall, the results of this study provide valuable information for elucidating the JA biosynthesis pathway in <i>S. miltiorrhiza</i> by comprehensive and methodical examination. |
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| ISSN: | 1661-6596 1422-0067 |