Identification and Characterization of Jasmonic Acid Biosynthetic Genes in <i>Salvia miltiorrhiza</i> Bunge
Jasmonic acid (JA) is a vital plant hormone that performs a variety of critical functions for plants. <i>Salvia miltiorrhiza</i> Bunge (<i>S. miltiorrhiza</i>), also known as Danshen, is a renowned traditional Chinese medicinal herb. However, no thorough and systematic analys...
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| Format: | Article |
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MDPI AG
2022-08-01
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| Series: | International Journal of Molecular Sciences |
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| Online Access: | https://www.mdpi.com/1422-0067/23/16/9384 |
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| author | Xiaoshan Xue Runqing Li Caijuan Zhang Wenna Li Lin Li Suying Hu Junfeng Niu Xiaoyan Cao Donghao Wang Zhezhi Wang |
| author_facet | Xiaoshan Xue Runqing Li Caijuan Zhang Wenna Li Lin Li Suying Hu Junfeng Niu Xiaoyan Cao Donghao Wang Zhezhi Wang |
| author_sort | Xiaoshan Xue |
| collection | DOAJ |
| description | Jasmonic acid (JA) is a vital plant hormone that performs a variety of critical functions for plants. <i>Salvia miltiorrhiza</i> Bunge (<i>S. miltiorrhiza</i>), also known as Danshen, is a renowned traditional Chinese medicinal herb. However, no thorough and systematic analysis of JA biosynthesis genes in <i>S. miltiorrhiza</i> exists. Through genome-wide prediction and molecular cloning, 23 candidate genes related to JA biosynthesis were identified in <i>S. miltiorrhiza</i>. These genes belong to four families that encode lipoxygenase (LOX), allene oxide synthase (AOS), allene oxide cyclase (AOC), and 12-OPDA reductase3 (OPR3). It was discovered that the candidate genes for JA synthesis of <i>S. miltiorrhiza</i> were distinct and conserved, in contrast to related genes in other plants, by evaluating their genetic structures, protein characteristics, and phylogenetic trees. These genes displayed tissue-specific expression patterns concerning to methyl jasmonate (MeJA) and wound tests. Overall, the results of this study provide valuable information for elucidating the JA biosynthesis pathway in <i>S. miltiorrhiza</i> by comprehensive and methodical examination. |
| first_indexed | 2024-03-09T13:15:42Z |
| format | Article |
| id | doaj.art-a2ec4fc1cea94678a6748bbffe05fa33 |
| institution | Directory Open Access Journal |
| issn | 1661-6596 1422-0067 |
| language | English |
| last_indexed | 2024-03-09T13:15:42Z |
| publishDate | 2022-08-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | International Journal of Molecular Sciences |
| spelling | doaj.art-a2ec4fc1cea94678a6748bbffe05fa332023-11-30T21:36:47ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672022-08-012316938410.3390/ijms23169384Identification and Characterization of Jasmonic Acid Biosynthetic Genes in <i>Salvia miltiorrhiza</i> BungeXiaoshan Xue0Runqing Li1Caijuan Zhang2Wenna Li3Lin Li4Suying Hu5Junfeng Niu6Xiaoyan Cao7Donghao Wang8Zhezhi Wang9Key Laboratory of the Ministry of Education for Medicinal Resources and Natural Pharmaceutical Chemistry, National Engineering Laboratory for Resource Development of Endangered Crude Drugs in Northwest of China, Shaanxi Normal University, Xi’an 710062, ChinaKey Laboratory of the Ministry of Education for Medicinal Resources and Natural Pharmaceutical Chemistry, National Engineering Laboratory for Resource Development of Endangered Crude Drugs in Northwest of China, Shaanxi Normal University, Xi’an 710062, ChinaKey Laboratory of the Ministry of Education for Medicinal Resources and Natural Pharmaceutical Chemistry, National Engineering Laboratory for Resource Development of Endangered Crude Drugs in Northwest of China, Shaanxi Normal University, Xi’an 710062, ChinaKey Laboratory of the Ministry of Education for Medicinal Resources and Natural Pharmaceutical Chemistry, National Engineering Laboratory for Resource Development of Endangered Crude Drugs in Northwest of China, Shaanxi Normal University, Xi’an 710062, ChinaInstitute of Landscape Science, Taiyuan University, Taiyuan 030032, ChinaKey Laboratory of the Ministry of Education for Medicinal Resources and Natural Pharmaceutical Chemistry, National Engineering Laboratory for Resource Development of Endangered Crude Drugs in Northwest of China, Shaanxi Normal University, Xi’an 710062, ChinaKey Laboratory of the Ministry of Education for Medicinal Resources and Natural Pharmaceutical Chemistry, National Engineering Laboratory for Resource Development of Endangered Crude Drugs in Northwest of China, Shaanxi Normal University, Xi’an 710062, ChinaKey Laboratory of the Ministry of Education for Medicinal Resources and Natural Pharmaceutical Chemistry, National Engineering Laboratory for Resource Development of Endangered Crude Drugs in Northwest of China, Shaanxi Normal University, Xi’an 710062, ChinaKey Laboratory of the Ministry of Education for Medicinal Resources and Natural Pharmaceutical Chemistry, National Engineering Laboratory for Resource Development of Endangered Crude Drugs in Northwest of China, Shaanxi Normal University, Xi’an 710062, ChinaKey Laboratory of the Ministry of Education for Medicinal Resources and Natural Pharmaceutical Chemistry, National Engineering Laboratory for Resource Development of Endangered Crude Drugs in Northwest of China, Shaanxi Normal University, Xi’an 710062, ChinaJasmonic acid (JA) is a vital plant hormone that performs a variety of critical functions for plants. <i>Salvia miltiorrhiza</i> Bunge (<i>S. miltiorrhiza</i>), also known as Danshen, is a renowned traditional Chinese medicinal herb. However, no thorough and systematic analysis of JA biosynthesis genes in <i>S. miltiorrhiza</i> exists. Through genome-wide prediction and molecular cloning, 23 candidate genes related to JA biosynthesis were identified in <i>S. miltiorrhiza</i>. These genes belong to four families that encode lipoxygenase (LOX), allene oxide synthase (AOS), allene oxide cyclase (AOC), and 12-OPDA reductase3 (OPR3). It was discovered that the candidate genes for JA synthesis of <i>S. miltiorrhiza</i> were distinct and conserved, in contrast to related genes in other plants, by evaluating their genetic structures, protein characteristics, and phylogenetic trees. These genes displayed tissue-specific expression patterns concerning to methyl jasmonate (MeJA) and wound tests. Overall, the results of this study provide valuable information for elucidating the JA biosynthesis pathway in <i>S. miltiorrhiza</i> by comprehensive and methodical examination.https://www.mdpi.com/1422-0067/23/16/9384jasmonic acid<i>Salvia miltiorrhiza</i>secondary metabolismtraditional Chinese medicine |
| spellingShingle | Xiaoshan Xue Runqing Li Caijuan Zhang Wenna Li Lin Li Suying Hu Junfeng Niu Xiaoyan Cao Donghao Wang Zhezhi Wang Identification and Characterization of Jasmonic Acid Biosynthetic Genes in <i>Salvia miltiorrhiza</i> Bunge International Journal of Molecular Sciences jasmonic acid <i>Salvia miltiorrhiza</i> secondary metabolism traditional Chinese medicine |
| title | Identification and Characterization of Jasmonic Acid Biosynthetic Genes in <i>Salvia miltiorrhiza</i> Bunge |
| title_full | Identification and Characterization of Jasmonic Acid Biosynthetic Genes in <i>Salvia miltiorrhiza</i> Bunge |
| title_fullStr | Identification and Characterization of Jasmonic Acid Biosynthetic Genes in <i>Salvia miltiorrhiza</i> Bunge |
| title_full_unstemmed | Identification and Characterization of Jasmonic Acid Biosynthetic Genes in <i>Salvia miltiorrhiza</i> Bunge |
| title_short | Identification and Characterization of Jasmonic Acid Biosynthetic Genes in <i>Salvia miltiorrhiza</i> Bunge |
| title_sort | identification and characterization of jasmonic acid biosynthetic genes in i salvia miltiorrhiza i bunge |
| topic | jasmonic acid <i>Salvia miltiorrhiza</i> secondary metabolism traditional Chinese medicine |
| url | https://www.mdpi.com/1422-0067/23/16/9384 |
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