Biochanin-A attenuates neuropathic pain in diabetic rats
Background: Soya supplements are used in the treatment of neuropathic pain. Previous reports reveal that consumption of soy diet before nerve injury prevents the development of neuropathic pain in rats. Biochanin-A, a soy isoflavone, has a naturally occurring inhibitor of fatty acid amide hydrolase...
| Main Authors: | , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Elsevier
2016-10-01
|
| Series: | Journal of Ayurveda and Integrative Medicine |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S0975947616301243 |
| _version_ | 1818239874506948608 |
|---|---|
| author | Venkateswarlu Chundi Siva Reddy Challa Devala Rao Garikapati Giridhar Juvva Anusha Jampani Sree Harsha Pinnamaneni Sahithi Venigalla |
| author_facet | Venkateswarlu Chundi Siva Reddy Challa Devala Rao Garikapati Giridhar Juvva Anusha Jampani Sree Harsha Pinnamaneni Sahithi Venigalla |
| author_sort | Venkateswarlu Chundi |
| collection | DOAJ |
| description | Background: Soya supplements are used in the treatment of neuropathic pain. Previous reports reveal that consumption of soy diet before nerve injury prevents the development of neuropathic pain in rats. Biochanin-A, a soy isoflavone, has a naturally occurring inhibitor of fatty acid amide hydrolase (FAAH) that metabolized endocannabinoids.
Objective: The objective was to evaluate efficacy of biochanin-A in streptozotocin (STZ) induced neuropathic pain in rat model.
Materials and methods: Diabetes mellitus was induced by an injection of STZ at a dose of 45 mg/kg, i.v. into tail vein of male albino Wistar rats. Biochanin-A was dosed at 0.1, 1 and 5 mg/kg by intraperitoneal (i.p.) administration in diabetic neuropathic rats. Mechanical hyperalgesia and allodynia was measured using Randall–Selitto analgesymeter and manual von Frey filaments of increasing weights respectively. Paw withdrawal threshold (PWT) and percent PWT was determined with respect to both hyperalgesia and allodynia.
Results: Treatment of biochanin-A at three different levels of 0.1, 1 and 5 mg/kg had not significantly altered serum glucose levels throughout the treatment period. In hyperalgesia study, acute treatment with higher dose exhibited 51.1% reversal of paw withdrawal threshold (PWT) while with chronic treatment, efficacy declined to 22.5% reversal of PWT. In allodynia study, acute treatment reversed PWT by 79.4% while with chronic treatment, efficacy was raised to 88.2% reversal of PWT.
Conclusion: Biochanin-A demonstrated better efficacy in reversing mechanical allodynia than mechanical hyperalgesia. Biochanin-A could be a good drug candidate for further studies to establish the mechanism of attenuation of neuropathic pain. |
| first_indexed | 2024-12-12T13:04:29Z |
| format | Article |
| id | doaj.art-a2ecb9d778f949a68ad05bb364895a4f |
| institution | Directory Open Access Journal |
| issn | 0975-9476 |
| language | English |
| last_indexed | 2024-12-12T13:04:29Z |
| publishDate | 2016-10-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Journal of Ayurveda and Integrative Medicine |
| spelling | doaj.art-a2ecb9d778f949a68ad05bb364895a4f2022-12-22T00:23:43ZengElsevierJournal of Ayurveda and Integrative Medicine0975-94762016-10-017423123710.1016/j.jaim.2016.08.001Biochanin-A attenuates neuropathic pain in diabetic ratsVenkateswarlu Chundi0Siva Reddy Challa1Devala Rao Garikapati2Giridhar Juvva3Anusha Jampani4Sree Harsha Pinnamaneni5Sahithi Venigalla6Department of Zoology, PB Siddhartha College of Arts & Science, Siddhartha Nagar, Vijayawada 520010, Andhra Pradesh, IndiaDepartment of Pharmacology, KVSR Siddhartha College of Pharmaceutical Sciences, Vijayawada 520010, Andhra Pradesh, IndiaDepartment of Pharmacology, KVSR Siddhartha College of Pharmaceutical Sciences, Vijayawada 520010, Andhra Pradesh, IndiaDepartment of Pharmacology, KVSR Siddhartha College of Pharmaceutical Sciences, Vijayawada 520010, Andhra Pradesh, IndiaDepartment of Pharmacology, KVSR Siddhartha College of Pharmaceutical Sciences, Vijayawada 520010, Andhra Pradesh, IndiaDepartment of Pharmacology, KVSR Siddhartha College of Pharmaceutical Sciences, Vijayawada 520010, Andhra Pradesh, IndiaDepartment of Pharmacology, KVSR Siddhartha College of Pharmaceutical Sciences, Vijayawada 520010, Andhra Pradesh, IndiaBackground: Soya supplements are used in the treatment of neuropathic pain. Previous reports reveal that consumption of soy diet before nerve injury prevents the development of neuropathic pain in rats. Biochanin-A, a soy isoflavone, has a naturally occurring inhibitor of fatty acid amide hydrolase (FAAH) that metabolized endocannabinoids. Objective: The objective was to evaluate efficacy of biochanin-A in streptozotocin (STZ) induced neuropathic pain in rat model. Materials and methods: Diabetes mellitus was induced by an injection of STZ at a dose of 45 mg/kg, i.v. into tail vein of male albino Wistar rats. Biochanin-A was dosed at 0.1, 1 and 5 mg/kg by intraperitoneal (i.p.) administration in diabetic neuropathic rats. Mechanical hyperalgesia and allodynia was measured using Randall–Selitto analgesymeter and manual von Frey filaments of increasing weights respectively. Paw withdrawal threshold (PWT) and percent PWT was determined with respect to both hyperalgesia and allodynia. Results: Treatment of biochanin-A at three different levels of 0.1, 1 and 5 mg/kg had not significantly altered serum glucose levels throughout the treatment period. In hyperalgesia study, acute treatment with higher dose exhibited 51.1% reversal of paw withdrawal threshold (PWT) while with chronic treatment, efficacy declined to 22.5% reversal of PWT. In allodynia study, acute treatment reversed PWT by 79.4% while with chronic treatment, efficacy was raised to 88.2% reversal of PWT. Conclusion: Biochanin-A demonstrated better efficacy in reversing mechanical allodynia than mechanical hyperalgesia. Biochanin-A could be a good drug candidate for further studies to establish the mechanism of attenuation of neuropathic pain.http://www.sciencedirect.com/science/article/pii/S0975947616301243AllodyniaDiabetic neuropathyHyperalgesiaIsoflavoneStreptozotocinHyperglycemiaPaw withdrawal thresholdvon Frey filaments |
| spellingShingle | Venkateswarlu Chundi Siva Reddy Challa Devala Rao Garikapati Giridhar Juvva Anusha Jampani Sree Harsha Pinnamaneni Sahithi Venigalla Biochanin-A attenuates neuropathic pain in diabetic rats Journal of Ayurveda and Integrative Medicine Allodynia Diabetic neuropathy Hyperalgesia Isoflavone Streptozotocin Hyperglycemia Paw withdrawal threshold von Frey filaments |
| title | Biochanin-A attenuates neuropathic pain in diabetic rats |
| title_full | Biochanin-A attenuates neuropathic pain in diabetic rats |
| title_fullStr | Biochanin-A attenuates neuropathic pain in diabetic rats |
| title_full_unstemmed | Biochanin-A attenuates neuropathic pain in diabetic rats |
| title_short | Biochanin-A attenuates neuropathic pain in diabetic rats |
| title_sort | biochanin a attenuates neuropathic pain in diabetic rats |
| topic | Allodynia Diabetic neuropathy Hyperalgesia Isoflavone Streptozotocin Hyperglycemia Paw withdrawal threshold von Frey filaments |
| url | http://www.sciencedirect.com/science/article/pii/S0975947616301243 |
| work_keys_str_mv | AT venkateswarluchundi biochaninaattenuatesneuropathicpainindiabeticrats AT sivareddychalla biochaninaattenuatesneuropathicpainindiabeticrats AT devalaraogarikapati biochaninaattenuatesneuropathicpainindiabeticrats AT giridharjuvva biochaninaattenuatesneuropathicpainindiabeticrats AT anushajampani biochaninaattenuatesneuropathicpainindiabeticrats AT sreeharshapinnamaneni biochaninaattenuatesneuropathicpainindiabeticrats AT sahithivenigalla biochaninaattenuatesneuropathicpainindiabeticrats |