Circular RNA hsa_circ_0005519 contributes to acute kidney injury via sponging microRNA-98-5p
Abstract Background This study intends to explore the role and molecular mechanism of hsa_circ_0005519 in acute kidney injury (AKI). Methods We conducted reverse transcription-qPCR for human serum to determine levels of hsa_circ_0005519 in AKI patients and healthy controls. Hsa_circ_0005519 was inhi...
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| Format: | Article |
| Language: | English |
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BMC
2024-03-01
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| Series: | BMC Nephrology |
| Subjects: | |
| Online Access: | https://doi.org/10.1186/s12882-024-03544-8 |
| _version_ | 1797247419051671552 |
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| author | Linsen Jiang Manxin Huang Jun Ge Xuefeng Zhang Ye Liu Hang Liu Xiaoming Liu Lili Jiang |
| author_facet | Linsen Jiang Manxin Huang Jun Ge Xuefeng Zhang Ye Liu Hang Liu Xiaoming Liu Lili Jiang |
| author_sort | Linsen Jiang |
| collection | DOAJ |
| description | Abstract Background This study intends to explore the role and molecular mechanism of hsa_circ_0005519 in acute kidney injury (AKI). Methods We conducted reverse transcription-qPCR for human serum to determine levels of hsa_circ_0005519 in AKI patients and healthy controls. Hsa_circ_0005519 was inhibited for expression in HK-2 cells using specific siRNAs. A number of techniques, MTT and ELISA assays, were used to analyze the potential role of hsa_circ_0005519 in cell viability, oxidative stress, and inflammation of LPS-induced HK-2 cells. results The serum of patients with AKI exhibited a significant increase in hsa_circ_0005519 expression, compared with healthy controls. Hsa_circ_0005519 was knockdown by siRNA, and its knockdown led to cell viability increase in LPS-induced HK-2 cells. Inhibition of hsa_circ_0005519 can reverse the TNF-α, IL-6 and IL-1β increase in LPS-induced HK-2 cells. Inhibiting hsa_circ_0005519 led to downregulation of MPO and MDA levels. MiR-98-5p was a downstream miRNA for hsa_circ_0005519. MiR-98-5p can offset the effects of hsa_circ_0005519 on LPS-induced HK-2 cells. IFG1R was a target gene for miR-98-5p. Conclusions These findings indicate that the highly expressed hsa_circ_0005519 plays a promoting role in AKI. |
| first_indexed | 2024-04-24T19:58:23Z |
| format | Article |
| id | doaj.art-a2ed62c83981424c83d60efd8a4327b1 |
| institution | Directory Open Access Journal |
| issn | 1471-2369 |
| language | English |
| last_indexed | 2024-04-24T19:58:23Z |
| publishDate | 2024-03-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Nephrology |
| spelling | doaj.art-a2ed62c83981424c83d60efd8a4327b12024-03-24T12:13:02ZengBMCBMC Nephrology1471-23692024-03-0125111010.1186/s12882-024-03544-8Circular RNA hsa_circ_0005519 contributes to acute kidney injury via sponging microRNA-98-5pLinsen Jiang0Manxin Huang1Jun Ge2Xuefeng Zhang3Ye Liu4Hang Liu5Xiaoming Liu6Lili Jiang7Department of Nephrology, The Second Affiliated Hospital of Soochow UniversitySchool of Medicine, Jianghan UniversityDepartment of Nephrology, Yantai Affiliated Hospital of Binzhou Medical UniversityDepartment of Pharmacy, Yantai Affiliated Hospital of Binzhou Medical UniversityDepartment of Nephrology, Yantai Affiliated Hospital of Binzhou Medical UniversityDepartment of Nephrology, Yantai Affiliated Hospital of Binzhou Medical UniversityDepartment of Nephrology, Yantai Affiliated Hospital of Binzhou Medical UniversityDepartment of Nephrology, Youyang Tujia and Miao Autonomous County People’s HospitalAbstract Background This study intends to explore the role and molecular mechanism of hsa_circ_0005519 in acute kidney injury (AKI). Methods We conducted reverse transcription-qPCR for human serum to determine levels of hsa_circ_0005519 in AKI patients and healthy controls. Hsa_circ_0005519 was inhibited for expression in HK-2 cells using specific siRNAs. A number of techniques, MTT and ELISA assays, were used to analyze the potential role of hsa_circ_0005519 in cell viability, oxidative stress, and inflammation of LPS-induced HK-2 cells. results The serum of patients with AKI exhibited a significant increase in hsa_circ_0005519 expression, compared with healthy controls. Hsa_circ_0005519 was knockdown by siRNA, and its knockdown led to cell viability increase in LPS-induced HK-2 cells. Inhibition of hsa_circ_0005519 can reverse the TNF-α, IL-6 and IL-1β increase in LPS-induced HK-2 cells. Inhibiting hsa_circ_0005519 led to downregulation of MPO and MDA levels. MiR-98-5p was a downstream miRNA for hsa_circ_0005519. MiR-98-5p can offset the effects of hsa_circ_0005519 on LPS-induced HK-2 cells. IFG1R was a target gene for miR-98-5p. Conclusions These findings indicate that the highly expressed hsa_circ_0005519 plays a promoting role in AKI.https://doi.org/10.1186/s12882-024-03544-8CircRNAMiRNAAcute kidney injuryCell viabilityInflammation |
| spellingShingle | Linsen Jiang Manxin Huang Jun Ge Xuefeng Zhang Ye Liu Hang Liu Xiaoming Liu Lili Jiang Circular RNA hsa_circ_0005519 contributes to acute kidney injury via sponging microRNA-98-5p BMC Nephrology CircRNA MiRNA Acute kidney injury Cell viability Inflammation |
| title | Circular RNA hsa_circ_0005519 contributes to acute kidney injury via sponging microRNA-98-5p |
| title_full | Circular RNA hsa_circ_0005519 contributes to acute kidney injury via sponging microRNA-98-5p |
| title_fullStr | Circular RNA hsa_circ_0005519 contributes to acute kidney injury via sponging microRNA-98-5p |
| title_full_unstemmed | Circular RNA hsa_circ_0005519 contributes to acute kidney injury via sponging microRNA-98-5p |
| title_short | Circular RNA hsa_circ_0005519 contributes to acute kidney injury via sponging microRNA-98-5p |
| title_sort | circular rna hsa circ 0005519 contributes to acute kidney injury via sponging microrna 98 5p |
| topic | CircRNA MiRNA Acute kidney injury Cell viability Inflammation |
| url | https://doi.org/10.1186/s12882-024-03544-8 |
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