Comparative Study of Toll-Like Receptor Immunological Function in Fallopian Tube Epithelial Cell-Line OE-E6/E7 in the Presence of Specific Ligands and Sperm under In-Vitro Conditions
Background and Objective: Toll-like receptors (TLRs) located in the fallopian tube epithelial cells play a crucial role in the immunological response to sperm and pathogens. The present study aimed to compare the function and response of TLR3, TLR4, and TLR5 receptors in the presence of sperm under...
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| Format: | Article |
| Language: | fas |
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Golestan University of Medical Sciences
2023-03-01
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| Series: | مجله دانشگاه علوم پزشکی گرگان |
| Subjects: | |
| Online Access: | http://goums.ac.ir/journal/article-1-4223-en.pdf |
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| author | Fatemehsadat Amjadi Ensieh Salehi Zahra Zandieh |
| author_facet | Fatemehsadat Amjadi Ensieh Salehi Zahra Zandieh |
| author_sort | Fatemehsadat Amjadi |
| collection | DOAJ |
| description | Background and Objective: Toll-like receptors (TLRs) located in the fallopian tube epithelial cells play a crucial role in the immunological response to sperm and pathogens. The present study aimed to compare the function and response of TLR3, TLR4, and TLR5 receptors in the presence of sperm under both physiological and pathological conditions in vitro.
Methods: In this descriptive laboratory study, OE-E6/E7 cells were cultured with fresh sperm samples obtained from normozoospermic individuals (n=10) and specific ligands for TLR3, TLR4, and TLR5 receptors in three groups consisting of sperm, specific ligands, and sperm + specific ligands. A control group was also included without adding sperm or ligand. The concentrations of IL-6 and IL-8 secreted from OE-E6/E7 cells in all four groups were determined using the ELISA method.
Results: Exposure of sperm and specific ligands to TLR3, TLR4, and TLR5 receptors in fallopian tube epithelial cells led to a significant increase in the concentration of IL-6 and IL-8 cytokines. There was no significant difference in the secretion of these cytokines from OE-E6/E7 cells between the two groups of ligand and ligand + sperm.
Conclusion: The response of fallopian tube epithelial cells to sperm exposure through TLRs leads to an increase in cytokine secretion. However, simultaneous exposure of sperm and TLR-specific ligands does not result in a cumulative increase in cytokine secretion. Therefore, it is plausible that the TLR signaling pathway may be regulated negatively by some other factors. Further studies are required to investigate this issue. |
| first_indexed | 2024-03-13T03:30:37Z |
| format | Article |
| id | doaj.art-a2ef3257779a4b80aead8721fc50ccee |
| institution | Directory Open Access Journal |
| issn | 1562-4765 2008-4080 |
| language | fas |
| last_indexed | 2024-03-13T03:30:37Z |
| publishDate | 2023-03-01 |
| publisher | Golestan University of Medical Sciences |
| record_format | Article |
| series | مجله دانشگاه علوم پزشکی گرگان |
| spelling | doaj.art-a2ef3257779a4b80aead8721fc50ccee2023-06-24T05:56:27ZfasGolestan University of Medical Sciencesمجله دانشگاه علوم پزشکی گرگان1562-47652008-40802023-03-012519298Comparative Study of Toll-Like Receptor Immunological Function in Fallopian Tube Epithelial Cell-Line OE-E6/E7 in the Presence of Specific Ligands and Sperm under In-Vitro ConditionsFatemehsadat Amjadi0Ensieh Salehi1Zahra Zandieh2 Ph.D of Reproductive Biology, Reproductive Sciences and Technology Research Center, Department of Anatomy, School of Medicine, Iran University of Medical Sciences, Tehran, Iran and Assistant Professor, Department of Anatomical Sciences, Iran University of Medical Sciences, Tehran, Iran. Ph.D of Reproductive Biology, Assistant Professor, Fertility and Infertility Research Center, Hormozgan University of Medical Sciences, Bandar Abbas, Iran. * Corresponding Author, Ph.D of Reproductive Biology, Reproductive Sciences and Technology Research Center, Department of Anatomy, School of Medicine, Iran University of Medical Sciences, Tehran, Iran and Assistant Professor, Department of Anatomical Sciences, Iran University of Medical Sciences, Tehran, Iran. Background and Objective: Toll-like receptors (TLRs) located in the fallopian tube epithelial cells play a crucial role in the immunological response to sperm and pathogens. The present study aimed to compare the function and response of TLR3, TLR4, and TLR5 receptors in the presence of sperm under both physiological and pathological conditions in vitro. Methods: In this descriptive laboratory study, OE-E6/E7 cells were cultured with fresh sperm samples obtained from normozoospermic individuals (n=10) and specific ligands for TLR3, TLR4, and TLR5 receptors in three groups consisting of sperm, specific ligands, and sperm + specific ligands. A control group was also included without adding sperm or ligand. The concentrations of IL-6 and IL-8 secreted from OE-E6/E7 cells in all four groups were determined using the ELISA method. Results: Exposure of sperm and specific ligands to TLR3, TLR4, and TLR5 receptors in fallopian tube epithelial cells led to a significant increase in the concentration of IL-6 and IL-8 cytokines. There was no significant difference in the secretion of these cytokines from OE-E6/E7 cells between the two groups of ligand and ligand + sperm. Conclusion: The response of fallopian tube epithelial cells to sperm exposure through TLRs leads to an increase in cytokine secretion. However, simultaneous exposure of sperm and TLR-specific ligands does not result in a cumulative increase in cytokine secretion. Therefore, it is plausible that the TLR signaling pathway may be regulated negatively by some other factors. Further studies are required to investigate this issue.http://goums.ac.ir/journal/article-1-4223-en.pdffallopian tubespermatozoatoll-like receptors |
| spellingShingle | Fatemehsadat Amjadi Ensieh Salehi Zahra Zandieh Comparative Study of Toll-Like Receptor Immunological Function in Fallopian Tube Epithelial Cell-Line OE-E6/E7 in the Presence of Specific Ligands and Sperm under In-Vitro Conditions مجله دانشگاه علوم پزشکی گرگان fallopian tube spermatozoa toll-like receptors |
| title | Comparative Study of Toll-Like Receptor Immunological Function in Fallopian Tube Epithelial Cell-Line OE-E6/E7 in the Presence of Specific Ligands and Sperm under In-Vitro Conditions |
| title_full | Comparative Study of Toll-Like Receptor Immunological Function in Fallopian Tube Epithelial Cell-Line OE-E6/E7 in the Presence of Specific Ligands and Sperm under In-Vitro Conditions |
| title_fullStr | Comparative Study of Toll-Like Receptor Immunological Function in Fallopian Tube Epithelial Cell-Line OE-E6/E7 in the Presence of Specific Ligands and Sperm under In-Vitro Conditions |
| title_full_unstemmed | Comparative Study of Toll-Like Receptor Immunological Function in Fallopian Tube Epithelial Cell-Line OE-E6/E7 in the Presence of Specific Ligands and Sperm under In-Vitro Conditions |
| title_short | Comparative Study of Toll-Like Receptor Immunological Function in Fallopian Tube Epithelial Cell-Line OE-E6/E7 in the Presence of Specific Ligands and Sperm under In-Vitro Conditions |
| title_sort | comparative study of toll like receptor immunological function in fallopian tube epithelial cell line oe e6 e7 in the presence of specific ligands and sperm under in vitro conditions |
| topic | fallopian tube spermatozoa toll-like receptors |
| url | http://goums.ac.ir/journal/article-1-4223-en.pdf |
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