The Preventive Effect of L-Lysine on Lysozyme Glycation in Type 2 Diabetes
Lysozyme is a bactericidal enzyme whose structure and functions change in diabetes. Chemical chaperones are small molecules including polyamines (e.g. spermine), amino acids (e.g. L-lysine) and polyols (e.g. glycerol). They can improve protein conformation in several stressful conditions such as gly...
| Main Authors: | , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Tehran University of Medical Sciences
2016-02-01
|
| Series: | Acta Medica Iranica |
| Subjects: | |
| Online Access: | https://acta.tums.ac.ir/index.php/acta/article/view/5385 |
| _version_ | 1818059022924775424 |
|---|---|
| author | Hossein Mirmiranpour shahnaz khaghani S. Zahra Bathaie Manouchehr Nakhjavani Abbas Kebriaeezadeh Maryam Ebadi Siavash Gerayesh-Nejad Mohammad Zangooei |
| author_facet | Hossein Mirmiranpour shahnaz khaghani S. Zahra Bathaie Manouchehr Nakhjavani Abbas Kebriaeezadeh Maryam Ebadi Siavash Gerayesh-Nejad Mohammad Zangooei |
| author_sort | Hossein Mirmiranpour |
| collection | DOAJ |
| description | Lysozyme is a bactericidal enzyme whose structure and functions change in diabetes. Chemical chaperones are small molecules including polyamines (e.g. spermine), amino acids (e.g. L-lysine) and polyols (e.g. glycerol). They can improve protein conformation in several stressful conditions such as glycation. In this study, the authors aimed to observe the effect of L-lysine as a chemical chaperone on structure and function of glycated lysozyme. In this study, in vitro and in vivo effects of L-lysine on lysozyme glycation were investigated. Lysozyme was incubated with glucose and/or L-lysine, followed by an investigation of its structure by electrophoresis, fluorescence spectroscopy, and circular dichroism spectroscopy and also assessment of its bactericidal activity against M. lysodeikticus. In the clinical trial, patients with type 2 diabetes mellitus (T2DM) were randomly divided into two groups of 25 (test and control). All patients received metformin and glibenclamide for a three months period. The test group was supplemented with 3 g/day of L-lysine. The quantity and activity of lysozyme and other parameters were then measured. Among the test group, L-lysine was found to reduce the advanced glycation end products (AGEs) in the sera of patients with T2DM and in vitro condition. This chemical chaperone reversed the alteration in lysozyme structure and function due to glycation and resulted in increased lysozyme activity. Structure and function of glycated lysozyme are significantly improved by l-lysine; therefore it can be considered an effective therapeutic supplementation in T2DM, decreasing the risk of infection in these patients. |
| first_indexed | 2024-12-10T13:09:55Z |
| format | Article |
| id | doaj.art-a2f18094a7794f4ab07ee48949053641 |
| institution | Directory Open Access Journal |
| issn | 0044-6025 1735-9694 |
| language | English |
| last_indexed | 2024-12-10T13:09:55Z |
| publishDate | 2016-02-01 |
| publisher | Tehran University of Medical Sciences |
| record_format | Article |
| series | Acta Medica Iranica |
| spelling | doaj.art-a2f18094a7794f4ab07ee489490536412022-12-22T01:47:42ZengTehran University of Medical SciencesActa Medica Iranica0044-60251735-96942016-02-015414946The Preventive Effect of L-Lysine on Lysozyme Glycation in Type 2 DiabetesHossein Mirmiranpour0shahnaz khaghani1S. Zahra Bathaie2Manouchehr Nakhjavani3Abbas Kebriaeezadeh4Maryam Ebadi5Siavash Gerayesh-Nejad6Mohammad Zangooei7Department of Biochemistry, School of Medicine, Alborz University of Medical Sciences, Karaj, Iran. AND Department of Clinical Biochemistry, School of Medicine, Tehran University of Medical Sciences, Tehran, Iran.Department of Clinical Biochemistry, School of Medicine, Tehran University of Medical Sciences, Tehran, Iran.Department of Clinical Biochemistry, School of Medical Sciences, Tarbiat Modares University, Tehran, Iran.Endocrinology and Metabolism Research Center, Vali-Asr Hospital, Tehran University of Medical Sciences, Tehran, Iran.Departments of Toxicology/ Pharmacology, School of Pharmacy, Tehran University of Medical Sciences, Tehran, Iran.Endocrinology and Metabolism Research Center, Vali-Asr Hospital, Tehran University of Medical Sciences, Tehran, Iran.Department of Clinical Biochemistry, School of Medicine, Tehran University of Medical Sciences, Tehran, Iran.Department of Clinical Biochemistry, School of Medicine, Tehran University of Medical Sciences, Tehran, Iran.Lysozyme is a bactericidal enzyme whose structure and functions change in diabetes. Chemical chaperones are small molecules including polyamines (e.g. spermine), amino acids (e.g. L-lysine) and polyols (e.g. glycerol). They can improve protein conformation in several stressful conditions such as glycation. In this study, the authors aimed to observe the effect of L-lysine as a chemical chaperone on structure and function of glycated lysozyme. In this study, in vitro and in vivo effects of L-lysine on lysozyme glycation were investigated. Lysozyme was incubated with glucose and/or L-lysine, followed by an investigation of its structure by electrophoresis, fluorescence spectroscopy, and circular dichroism spectroscopy and also assessment of its bactericidal activity against M. lysodeikticus. In the clinical trial, patients with type 2 diabetes mellitus (T2DM) were randomly divided into two groups of 25 (test and control). All patients received metformin and glibenclamide for a three months period. The test group was supplemented with 3 g/day of L-lysine. The quantity and activity of lysozyme and other parameters were then measured. Among the test group, L-lysine was found to reduce the advanced glycation end products (AGEs) in the sera of patients with T2DM and in vitro condition. This chemical chaperone reversed the alteration in lysozyme structure and function due to glycation and resulted in increased lysozyme activity. Structure and function of glycated lysozyme are significantly improved by l-lysine; therefore it can be considered an effective therapeutic supplementation in T2DM, decreasing the risk of infection in these patients.https://acta.tums.ac.ir/index.php/acta/article/view/5385LysozymeChemical chaperoneL-lysineType 2 diabetes |
| spellingShingle | Hossein Mirmiranpour shahnaz khaghani S. Zahra Bathaie Manouchehr Nakhjavani Abbas Kebriaeezadeh Maryam Ebadi Siavash Gerayesh-Nejad Mohammad Zangooei The Preventive Effect of L-Lysine on Lysozyme Glycation in Type 2 Diabetes Acta Medica Iranica Lysozyme Chemical chaperone L-lysine Type 2 diabetes |
| title | The Preventive Effect of L-Lysine on Lysozyme Glycation in Type 2 Diabetes |
| title_full | The Preventive Effect of L-Lysine on Lysozyme Glycation in Type 2 Diabetes |
| title_fullStr | The Preventive Effect of L-Lysine on Lysozyme Glycation in Type 2 Diabetes |
| title_full_unstemmed | The Preventive Effect of L-Lysine on Lysozyme Glycation in Type 2 Diabetes |
| title_short | The Preventive Effect of L-Lysine on Lysozyme Glycation in Type 2 Diabetes |
| title_sort | preventive effect of l lysine on lysozyme glycation in type 2 diabetes |
| topic | Lysozyme Chemical chaperone L-lysine Type 2 diabetes |
| url | https://acta.tums.ac.ir/index.php/acta/article/view/5385 |
| work_keys_str_mv | AT hosseinmirmiranpour thepreventiveeffectofllysineonlysozymeglycationintype2diabetes AT shahnazkhaghani thepreventiveeffectofllysineonlysozymeglycationintype2diabetes AT szahrabathaie thepreventiveeffectofllysineonlysozymeglycationintype2diabetes AT manouchehrnakhjavani thepreventiveeffectofllysineonlysozymeglycationintype2diabetes AT abbaskebriaeezadeh thepreventiveeffectofllysineonlysozymeglycationintype2diabetes AT maryamebadi thepreventiveeffectofllysineonlysozymeglycationintype2diabetes AT siavashgerayeshnejad thepreventiveeffectofllysineonlysozymeglycationintype2diabetes AT mohammadzangooei thepreventiveeffectofllysineonlysozymeglycationintype2diabetes AT hosseinmirmiranpour preventiveeffectofllysineonlysozymeglycationintype2diabetes AT shahnazkhaghani preventiveeffectofllysineonlysozymeglycationintype2diabetes AT szahrabathaie preventiveeffectofllysineonlysozymeglycationintype2diabetes AT manouchehrnakhjavani preventiveeffectofllysineonlysozymeglycationintype2diabetes AT abbaskebriaeezadeh preventiveeffectofllysineonlysozymeglycationintype2diabetes AT maryamebadi preventiveeffectofllysineonlysozymeglycationintype2diabetes AT siavashgerayeshnejad preventiveeffectofllysineonlysozymeglycationintype2diabetes AT mohammadzangooei preventiveeffectofllysineonlysozymeglycationintype2diabetes |