Use of direct LAMP screening of broiler fecal samples for Campylobacter jejuni and Campylobacter coli in the positive flock identification strategy
Rapid identification of Campylobacter-positive flocks before slaughter, following freezing and heat treatment for the Campylobacter-positive carcasses at the slaughterhouses is an effective control strategy against foodborne campylobacteriosis. We evaluated a loop-mediated isothermal amplification (...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2016-09-01
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| Series: | Frontiers in Microbiology |
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| Online Access: | http://journal.frontiersin.org/Journal/10.3389/fmicb.2016.01582/full |
| _version_ | 1819292886342041600 |
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| author | Islam Ibrahim Sabike Islam Ibrahim Sabike Ryoko Uemura Ryoko Uemura Yumi Kirino Hirohisa Mekata Hirohisa Mekata Hirohisa Mekata Satoshi Sekiguchi Satoshi Sekiguchi Tamaki Okabayashi Tamaki Okabayashi Yoshitaka Goto Yoshitaka Goto Wataru Yamazaki Wataru Yamazaki |
| author_facet | Islam Ibrahim Sabike Islam Ibrahim Sabike Ryoko Uemura Ryoko Uemura Yumi Kirino Hirohisa Mekata Hirohisa Mekata Hirohisa Mekata Satoshi Sekiguchi Satoshi Sekiguchi Tamaki Okabayashi Tamaki Okabayashi Yoshitaka Goto Yoshitaka Goto Wataru Yamazaki Wataru Yamazaki |
| author_sort | Islam Ibrahim Sabike |
| collection | DOAJ |
| description | Rapid identification of Campylobacter-positive flocks before slaughter, following freezing and heat treatment for the Campylobacter-positive carcasses at the slaughterhouses is an effective control strategy against foodborne campylobacteriosis. We evaluated a loop-mediated isothermal amplification (LAMP) assay for the direct screening of naturally contaminated chicken cloacal swabs for Campylobacter jejuni/Campylobacter coli to compare this assay with conventional quantitative culture methods. In a comparison study of 165 broilers, the LAMP assay showed 82.8% (48/58 by conventional culture) sensitivity, 100% (107/107) specificity, 100% (48/48) positive predictive value (PPV), and 91.5% (107/117) negative predictive value (NPV). In a comparison of 55 flocks, LAMP showed 90.5% (19/21) sensitivity, 100% (34/34) specificity, 100% (19/19) PPV, and 94.4% (34/36) NPV. In the cumulative total of 28 farm-level comparisons, LAMP showed 100% (12/12) sensitivity, 100% (16/16) specificity, 100% (12/12) PPV, and 100% (16/16) NPV. The LAMP assay required less than 90 min from the arrival of the fecal samples to final results in the laboratory. This suggests that the LAMP assay will facilitate the identification of C. jejuni/C. coli-positive broiler flocks at the farm level or in slaughterhouses before slaughtering, which would make it an effective tool in preventing the spread of Campylobacter contamination. |
| first_indexed | 2024-12-24T04:01:39Z |
| format | Article |
| id | doaj.art-a2f44bfddc494242b44c46ac6b05b208 |
| institution | Directory Open Access Journal |
| issn | 1664-302X |
| language | English |
| last_indexed | 2024-12-24T04:01:39Z |
| publishDate | 2016-09-01 |
| publisher | Frontiers Media S.A. |
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| series | Frontiers in Microbiology |
| spelling | doaj.art-a2f44bfddc494242b44c46ac6b05b2082022-12-21T17:16:19ZengFrontiers Media S.A.Frontiers in Microbiology1664-302X2016-09-01710.3389/fmicb.2016.01582219962Use of direct LAMP screening of broiler fecal samples for Campylobacter jejuni and Campylobacter coli in the positive flock identification strategyIslam Ibrahim Sabike0Islam Ibrahim Sabike1Ryoko Uemura2Ryoko Uemura3Yumi Kirino4Hirohisa Mekata5Hirohisa Mekata6Hirohisa Mekata7Satoshi Sekiguchi8Satoshi Sekiguchi9Tamaki Okabayashi10Tamaki Okabayashi11Yoshitaka Goto12Yoshitaka Goto13Wataru Yamazaki14Wataru Yamazaki15University of MiyazakiBenha UniversityUniversity of MiyazakiUniversity of MiyazakiUniversity of MiyazakiUniversity of MiyazakiUniversity of Miyazaki, MiyazakiUniversity of MiyazakiUniversity of MiyazakiUniversity of MiyazakiUniversity of MiyazakiUniversity of MiyazakiUniversity of MiyazakiUniversity of MiyazakiUniversity of MiyazakiUniversity of MiyazakiRapid identification of Campylobacter-positive flocks before slaughter, following freezing and heat treatment for the Campylobacter-positive carcasses at the slaughterhouses is an effective control strategy against foodborne campylobacteriosis. We evaluated a loop-mediated isothermal amplification (LAMP) assay for the direct screening of naturally contaminated chicken cloacal swabs for Campylobacter jejuni/Campylobacter coli to compare this assay with conventional quantitative culture methods. In a comparison study of 165 broilers, the LAMP assay showed 82.8% (48/58 by conventional culture) sensitivity, 100% (107/107) specificity, 100% (48/48) positive predictive value (PPV), and 91.5% (107/117) negative predictive value (NPV). In a comparison of 55 flocks, LAMP showed 90.5% (19/21) sensitivity, 100% (34/34) specificity, 100% (19/19) PPV, and 94.4% (34/36) NPV. In the cumulative total of 28 farm-level comparisons, LAMP showed 100% (12/12) sensitivity, 100% (16/16) specificity, 100% (12/12) PPV, and 100% (16/16) NPV. The LAMP assay required less than 90 min from the arrival of the fecal samples to final results in the laboratory. This suggests that the LAMP assay will facilitate the identification of C. jejuni/C. coli-positive broiler flocks at the farm level or in slaughterhouses before slaughtering, which would make it an effective tool in preventing the spread of Campylobacter contamination.http://journal.frontiersin.org/Journal/10.3389/fmicb.2016.01582/fullCampylobacter coliCampylobacter jejuniChickensFecesfoodborneLAMP |
| spellingShingle | Islam Ibrahim Sabike Islam Ibrahim Sabike Ryoko Uemura Ryoko Uemura Yumi Kirino Hirohisa Mekata Hirohisa Mekata Hirohisa Mekata Satoshi Sekiguchi Satoshi Sekiguchi Tamaki Okabayashi Tamaki Okabayashi Yoshitaka Goto Yoshitaka Goto Wataru Yamazaki Wataru Yamazaki Use of direct LAMP screening of broiler fecal samples for Campylobacter jejuni and Campylobacter coli in the positive flock identification strategy Frontiers in Microbiology Campylobacter coli Campylobacter jejuni Chickens Feces foodborne LAMP |
| title | Use of direct LAMP screening of broiler fecal samples for Campylobacter jejuni and Campylobacter coli in the positive flock identification strategy |
| title_full | Use of direct LAMP screening of broiler fecal samples for Campylobacter jejuni and Campylobacter coli in the positive flock identification strategy |
| title_fullStr | Use of direct LAMP screening of broiler fecal samples for Campylobacter jejuni and Campylobacter coli in the positive flock identification strategy |
| title_full_unstemmed | Use of direct LAMP screening of broiler fecal samples for Campylobacter jejuni and Campylobacter coli in the positive flock identification strategy |
| title_short | Use of direct LAMP screening of broiler fecal samples for Campylobacter jejuni and Campylobacter coli in the positive flock identification strategy |
| title_sort | use of direct lamp screening of broiler fecal samples for campylobacter jejuni and campylobacter coli in the positive flock identification strategy |
| topic | Campylobacter coli Campylobacter jejuni Chickens Feces foodborne LAMP |
| url | http://journal.frontiersin.org/Journal/10.3389/fmicb.2016.01582/full |
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