Rapid On-Site Detection of SARS-CoV-2 Using RT-LAMP Assay with a Portable Low-Cost Device
Emerging infectious diseases pose a serious threat to human health and affect social stability. In recent years, the epidemic situation of emerging infectious diseases is very serious; among these infectious diseases, severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has affected many cou...
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| Format: | Article |
| Language: | English |
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MDPI AG
2023-07-01
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| Series: | Biosensors |
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| Online Access: | https://www.mdpi.com/2079-6374/13/7/724 |
| _version_ | 1797590046785667072 |
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| author | Quanying Fu Xueyuan Pang Zhenning Su Yuxiao Yang Yiren Liu Ziyue Zhang Yuqiu Fu Jiasi Wang Jianhua Zhou |
| author_facet | Quanying Fu Xueyuan Pang Zhenning Su Yuxiao Yang Yiren Liu Ziyue Zhang Yuqiu Fu Jiasi Wang Jianhua Zhou |
| author_sort | Quanying Fu |
| collection | DOAJ |
| description | Emerging infectious diseases pose a serious threat to human health and affect social stability. In recent years, the epidemic situation of emerging infectious diseases is very serious; among these infectious diseases, severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has affected many countries and regions in a short time. The prevention and treatment of these diseases require rapid on-site detection methods. However, the common detection method, RT-PCR, requires expensive instruments, complex operations, and professional operators. Here, we developed a portable low-cost assay for rapid on-site detection of viral nucleic acid using reverse transcription-loop-mediated isothermal amplification (RT-LAMP). The SARS-CoV-2 RNA can be successfully amplified within 15 min in a thermos, and the detection result is read rapidly in a portable low-cost device with a sensitivity of 10<sup>0</sup> copies/µL. The portable low-cost device consists of a black box, a laser or LED and a filter, costing only a few cents. The rapid on-site detection method can provide strong support for the control of biological threats such as infectious diseases. It is also an emergency detection method for low-resource settings, relieving the huge pressure on health care. |
| first_indexed | 2024-03-11T01:15:50Z |
| format | Article |
| id | doaj.art-a2fabd9751d144689f0c1d194fa48175 |
| institution | Directory Open Access Journal |
| issn | 2079-6374 |
| language | English |
| last_indexed | 2024-03-11T01:15:50Z |
| publishDate | 2023-07-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Biosensors |
| spelling | doaj.art-a2fabd9751d144689f0c1d194fa481752023-11-18T18:33:07ZengMDPI AGBiosensors2079-63742023-07-0113772410.3390/bios13070724Rapid On-Site Detection of SARS-CoV-2 Using RT-LAMP Assay with a Portable Low-Cost DeviceQuanying Fu0Xueyuan Pang1Zhenning Su2Yuxiao Yang3Yiren Liu4Ziyue Zhang5Yuqiu Fu6Jiasi Wang7Jianhua Zhou8Guangdong Provincial Key Laboratory of Sensing Technology and Biomedical Instrument, School of Biomedical Engineering, Shenzhen Campus of Sun Yat-sen University, Shenzhen 518107, ChinaGuangdong Provincial Key Laboratory of Sensing Technology and Biomedical Instrument, School of Biomedical Engineering, Shenzhen Campus of Sun Yat-sen University, Shenzhen 518107, ChinaGuangdong Provincial Key Laboratory of Sensing Technology and Biomedical Instrument, School of Biomedical Engineering, Sun Yat-sen University, Guangzhou 510275, ChinaGuangdong Provincial Key Laboratory of Sensing Technology and Biomedical Instrument, School of Biomedical Engineering, Shenzhen Campus of Sun Yat-sen University, Shenzhen 518107, ChinaGuangdong Provincial Key Laboratory of Sensing Technology and Biomedical Instrument, School of Biomedical Engineering, Shenzhen Campus of Sun Yat-sen University, Shenzhen 518107, ChinaGuangdong Provincial Key Laboratory of Sensing Technology and Biomedical Instrument, School of Biomedical Engineering, Shenzhen Campus of Sun Yat-sen University, Shenzhen 518107, ChinaGuangdong Provincial Key Laboratory of Sensing Technology and Biomedical Instrument, School of Biomedical Engineering, Shenzhen Campus of Sun Yat-sen University, Shenzhen 518107, ChinaGuangdong Provincial Key Laboratory of Sensing Technology and Biomedical Instrument, School of Biomedical Engineering, Shenzhen Campus of Sun Yat-sen University, Shenzhen 518107, ChinaGuangdong Provincial Key Laboratory of Sensing Technology and Biomedical Instrument, School of Biomedical Engineering, Shenzhen Campus of Sun Yat-sen University, Shenzhen 518107, ChinaEmerging infectious diseases pose a serious threat to human health and affect social stability. In recent years, the epidemic situation of emerging infectious diseases is very serious; among these infectious diseases, severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has affected many countries and regions in a short time. The prevention and treatment of these diseases require rapid on-site detection methods. However, the common detection method, RT-PCR, requires expensive instruments, complex operations, and professional operators. Here, we developed a portable low-cost assay for rapid on-site detection of viral nucleic acid using reverse transcription-loop-mediated isothermal amplification (RT-LAMP). The SARS-CoV-2 RNA can be successfully amplified within 15 min in a thermos, and the detection result is read rapidly in a portable low-cost device with a sensitivity of 10<sup>0</sup> copies/µL. The portable low-cost device consists of a black box, a laser or LED and a filter, costing only a few cents. The rapid on-site detection method can provide strong support for the control of biological threats such as infectious diseases. It is also an emergency detection method for low-resource settings, relieving the huge pressure on health care.https://www.mdpi.com/2079-6374/13/7/724rapid on-site detectionportable low-cost devicenucleic acid detectionemerging infectious diseasesSARS-CoV-2 RNA |
| spellingShingle | Quanying Fu Xueyuan Pang Zhenning Su Yuxiao Yang Yiren Liu Ziyue Zhang Yuqiu Fu Jiasi Wang Jianhua Zhou Rapid On-Site Detection of SARS-CoV-2 Using RT-LAMP Assay with a Portable Low-Cost Device Biosensors rapid on-site detection portable low-cost device nucleic acid detection emerging infectious diseases SARS-CoV-2 RNA |
| title | Rapid On-Site Detection of SARS-CoV-2 Using RT-LAMP Assay with a Portable Low-Cost Device |
| title_full | Rapid On-Site Detection of SARS-CoV-2 Using RT-LAMP Assay with a Portable Low-Cost Device |
| title_fullStr | Rapid On-Site Detection of SARS-CoV-2 Using RT-LAMP Assay with a Portable Low-Cost Device |
| title_full_unstemmed | Rapid On-Site Detection of SARS-CoV-2 Using RT-LAMP Assay with a Portable Low-Cost Device |
| title_short | Rapid On-Site Detection of SARS-CoV-2 Using RT-LAMP Assay with a Portable Low-Cost Device |
| title_sort | rapid on site detection of sars cov 2 using rt lamp assay with a portable low cost device |
| topic | rapid on-site detection portable low-cost device nucleic acid detection emerging infectious diseases SARS-CoV-2 RNA |
| url | https://www.mdpi.com/2079-6374/13/7/724 |
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