Metabolism of eriocitrin in the gut and its regulation on gut microbiota in mice
IntroductionEriocitrin, found in lemon fruit, has shown a wide range of biological properties. Herein, we investigated the intestinal metabolic profile of eriocitrin in colon, and the regulation of dietary intervention of eriocitrin on gut microbiota.MethodsWe performed ultra performance liquid chro...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2023-01-01
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| Series: | Frontiers in Microbiology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fmicb.2022.1111200/full |
| _version_ | 1797955370761584640 |
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| author | Xia Meng Hongchen Wu Jiayi Xiong Yongquan Li Lin Chen Qing Gu Ping Li |
| author_facet | Xia Meng Hongchen Wu Jiayi Xiong Yongquan Li Lin Chen Qing Gu Ping Li |
| author_sort | Xia Meng |
| collection | DOAJ |
| description | IntroductionEriocitrin, found in lemon fruit, has shown a wide range of biological properties. Herein, we investigated the intestinal metabolic profile of eriocitrin in colon, and the regulation of dietary intervention of eriocitrin on gut microbiota.MethodsWe performed ultra performance liquid chromatography-electrospray ionization-tandem mass spectrometry (UPLC-ESI-MS/MS), 16S rDNA gene sequencing and gas chromatography-mass (GC-MS) on colon contents from the eriocitrin group (n=6), and compared them with control participants (n=6).ResultsA total of 136 flavonoids were found in colon contents, including eriocitrin and its six metabolites (eriodictyol, homoeriodictyol, hesperetin, eriodictyol-3′-O-glucoside, hesperetin-7-O-glucoside and eriodictyol-7-O-(6″-O-galloyl) glucoside). Moreover, dietary intervention of eriocitrin significantly alters the beta diversity of the gut microbiota, the probiotics such as Lachnospiraceae_UCG_006 were significantly enriched, and the production of butyrate, valerate and hexanoate in the colon pool of short-chain fatty acids were significant increased. The spearman’s association analysis performed some intestinal bacteria may be involved in the metabolism of eriocitrin.DiscussionCollectively, our results preliminarily suggest the metabolism of eriocitrin in the gut, demonstrating alterations of eriocitrin in gut microbiota, which warrants further investigation to determine its potential use in food and biomedical applications. |
| first_indexed | 2024-04-10T23:32:02Z |
| format | Article |
| id | doaj.art-a2fe5150efc343ada9d1ffbc4aa99e38 |
| institution | Directory Open Access Journal |
| issn | 1664-302X |
| language | English |
| last_indexed | 2024-04-10T23:32:02Z |
| publishDate | 2023-01-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Microbiology |
| spelling | doaj.art-a2fe5150efc343ada9d1ffbc4aa99e382023-01-12T05:15:10ZengFrontiers Media S.A.Frontiers in Microbiology1664-302X2023-01-011310.3389/fmicb.2022.11112001111200Metabolism of eriocitrin in the gut and its regulation on gut microbiota in miceXia MengHongchen WuJiayi XiongYongquan LiLin ChenQing GuPing LiIntroductionEriocitrin, found in lemon fruit, has shown a wide range of biological properties. Herein, we investigated the intestinal metabolic profile of eriocitrin in colon, and the regulation of dietary intervention of eriocitrin on gut microbiota.MethodsWe performed ultra performance liquid chromatography-electrospray ionization-tandem mass spectrometry (UPLC-ESI-MS/MS), 16S rDNA gene sequencing and gas chromatography-mass (GC-MS) on colon contents from the eriocitrin group (n=6), and compared them with control participants (n=6).ResultsA total of 136 flavonoids were found in colon contents, including eriocitrin and its six metabolites (eriodictyol, homoeriodictyol, hesperetin, eriodictyol-3′-O-glucoside, hesperetin-7-O-glucoside and eriodictyol-7-O-(6″-O-galloyl) glucoside). Moreover, dietary intervention of eriocitrin significantly alters the beta diversity of the gut microbiota, the probiotics such as Lachnospiraceae_UCG_006 were significantly enriched, and the production of butyrate, valerate and hexanoate in the colon pool of short-chain fatty acids were significant increased. The spearman’s association analysis performed some intestinal bacteria may be involved in the metabolism of eriocitrin.DiscussionCollectively, our results preliminarily suggest the metabolism of eriocitrin in the gut, demonstrating alterations of eriocitrin in gut microbiota, which warrants further investigation to determine its potential use in food and biomedical applications.https://www.frontiersin.org/articles/10.3389/fmicb.2022.1111200/fulleriocitrinmetabolismgut microbiotashort-chain fatty acidsdietary intervention |
| spellingShingle | Xia Meng Hongchen Wu Jiayi Xiong Yongquan Li Lin Chen Qing Gu Ping Li Metabolism of eriocitrin in the gut and its regulation on gut microbiota in mice Frontiers in Microbiology eriocitrin metabolism gut microbiota short-chain fatty acids dietary intervention |
| title | Metabolism of eriocitrin in the gut and its regulation on gut microbiota in mice |
| title_full | Metabolism of eriocitrin in the gut and its regulation on gut microbiota in mice |
| title_fullStr | Metabolism of eriocitrin in the gut and its regulation on gut microbiota in mice |
| title_full_unstemmed | Metabolism of eriocitrin in the gut and its regulation on gut microbiota in mice |
| title_short | Metabolism of eriocitrin in the gut and its regulation on gut microbiota in mice |
| title_sort | metabolism of eriocitrin in the gut and its regulation on gut microbiota in mice |
| topic | eriocitrin metabolism gut microbiota short-chain fatty acids dietary intervention |
| url | https://www.frontiersin.org/articles/10.3389/fmicb.2022.1111200/full |
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