Photo-Fenton and TiO<sub>2</sub> Photocatalytic Inactivation of Model Microorganisms under UV-A; Comparative Efficacy and Optimization
Photocatalytic inactivation of pathogens in aqueous waste is gaining increasing attention. Several homogeneous and heterogeneous photocatalytic protocols exist using the Fenton’s reagent and TiO<sub>2</sub>, respectively. A comprehensive study of homogeneous and heterogeneous photocataly...
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| Format: | Article |
| Language: | English |
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MDPI AG
2023-01-01
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| Series: | Molecules |
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| Online Access: | https://www.mdpi.com/1420-3049/28/3/1199 |
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| author | Eirini Kanata Ioannis Paspaltsis Sotiris Sotiriadis Chrysanthi Berberidou Sophia Tsoumachidou Dimitra Dafou Konstantinos Xanthopoulos Minas Arsenakis Athanasios Arsenakis Ioannis Poulios Theodoros Sklaviadis |
| author_facet | Eirini Kanata Ioannis Paspaltsis Sotiris Sotiriadis Chrysanthi Berberidou Sophia Tsoumachidou Dimitra Dafou Konstantinos Xanthopoulos Minas Arsenakis Athanasios Arsenakis Ioannis Poulios Theodoros Sklaviadis |
| author_sort | Eirini Kanata |
| collection | DOAJ |
| description | Photocatalytic inactivation of pathogens in aqueous waste is gaining increasing attention. Several homogeneous and heterogeneous photocatalytic protocols exist using the Fenton’s reagent and TiO<sub>2</sub>, respectively. A comprehensive study of homogeneous and heterogeneous photocatalysis on a range of microorganisms will significantly establish the most efficient method. Here, we report a comparative study of TiO<sub>2</sub>- and Fe<sup>+3</sup>-based photocatalytic inactivation under UV-A of diverse microorganisms, including Gram-positive (<i>Staphylococcus aureus</i>) and Gram-negative (<i>Escherichia coli</i>) bacteria, bacterial spores (<i>Bacillus stearothermophilus</i> spores) and viruses (MS2). We also present data on the optimization of TiO<sub>2</sub> photocatalysis, including optimal catalyst concentration and H<sub>2</sub>O<sub>2</sub> supplementation. Our results indicate that both photo-Fenton and TiO<sub>2</sub> could be successfully applied for the management of microbial loads in liquids. Efficient microorganism inactivation is achieved with homogeneous photocatalysis (7 mg/L Fe<sup>+3</sup>, 100 mg/L H<sub>2</sub>O<sub>2</sub>, UV-A) in a shorter processing time compared to heterogeneous photocatalysis (0.5 g/L TiO<sub>2</sub>, UV-A), whereas similar or shorter processing is required when heterogenous photocatalysis is performed using microorganism-specific optimized TiO<sub>2</sub> concentrations and H<sub>2</sub>O<sub>2</sub> supplementation (100 mg/L); higher H<sub>2</sub>O<sub>2</sub> concentrations further enhance the heterogenous photocatalytic inactivation efficiency. Our study provides a template protocol for the design and further application for large-scale photocatalytic approaches to inactivate pathogens in liquid biomedical waste. |
| first_indexed | 2024-03-11T09:33:35Z |
| format | Article |
| id | doaj.art-a2fefc1a61b047229274a0bb3dc5a569 |
| institution | Directory Open Access Journal |
| issn | 1420-3049 |
| language | English |
| last_indexed | 2024-03-11T09:33:35Z |
| publishDate | 2023-01-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Molecules |
| spelling | doaj.art-a2fefc1a61b047229274a0bb3dc5a5692023-11-16T17:29:04ZengMDPI AGMolecules1420-30492023-01-01283119910.3390/molecules28031199Photo-Fenton and TiO<sub>2</sub> Photocatalytic Inactivation of Model Microorganisms under UV-A; Comparative Efficacy and OptimizationEirini Kanata0Ioannis Paspaltsis1Sotiris Sotiriadis2Chrysanthi Berberidou3Sophia Tsoumachidou4Dimitra Dafou5Konstantinos Xanthopoulos6Minas Arsenakis7Athanasios Arsenakis8Ioannis Poulios9Theodoros Sklaviadis10Laboratory of Pharmacology, Department of Pharmacy, School of Health Sciences, Aristotle University of Thessaloniki, 54124 Thessaloniki, GreeceLaboratory of Pharmacology, Department of Pharmacy, School of Health Sciences, Aristotle University of Thessaloniki, 54124 Thessaloniki, GreeceDepartment of Genetics, Development and Molecular Biology, School of Biology, Aristotle University of Thessaloniki, 54124 Thessaloniki, GreeceLaboratory of Physical Chemistry, Department of Chemistry, Aristotle University of Thessaloniki, 54124 Thessaloniki, GreeceLaboratory of Physical Chemistry, Department of Chemistry, Aristotle University of Thessaloniki, 54124 Thessaloniki, GreeceDepartment of Genetics, Development and Molecular Biology, School of Biology, Aristotle University of Thessaloniki, 54124 Thessaloniki, GreeceLaboratory of Pharmacology, Department of Pharmacy, School of Health Sciences, Aristotle University of Thessaloniki, 54124 Thessaloniki, GreeceLaboratory of General Microbiology, Department of Genetics Development and Molecular Biology, School of Biology, Aristotle University of Thessaloniki, 54124 Thessaloniki, GreeceSTERIMED SA, G’ Fassi, Building Block 52b, Sindos Industrial Area, 57022 Thessaloniki, GreeceLaboratory of Physical Chemistry, Department of Chemistry, Aristotle University of Thessaloniki, 54124 Thessaloniki, GreeceLaboratory of Pharmacology, Department of Pharmacy, School of Health Sciences, Aristotle University of Thessaloniki, 54124 Thessaloniki, GreecePhotocatalytic inactivation of pathogens in aqueous waste is gaining increasing attention. Several homogeneous and heterogeneous photocatalytic protocols exist using the Fenton’s reagent and TiO<sub>2</sub>, respectively. A comprehensive study of homogeneous and heterogeneous photocatalysis on a range of microorganisms will significantly establish the most efficient method. Here, we report a comparative study of TiO<sub>2</sub>- and Fe<sup>+3</sup>-based photocatalytic inactivation under UV-A of diverse microorganisms, including Gram-positive (<i>Staphylococcus aureus</i>) and Gram-negative (<i>Escherichia coli</i>) bacteria, bacterial spores (<i>Bacillus stearothermophilus</i> spores) and viruses (MS2). We also present data on the optimization of TiO<sub>2</sub> photocatalysis, including optimal catalyst concentration and H<sub>2</sub>O<sub>2</sub> supplementation. Our results indicate that both photo-Fenton and TiO<sub>2</sub> could be successfully applied for the management of microbial loads in liquids. Efficient microorganism inactivation is achieved with homogeneous photocatalysis (7 mg/L Fe<sup>+3</sup>, 100 mg/L H<sub>2</sub>O<sub>2</sub>, UV-A) in a shorter processing time compared to heterogeneous photocatalysis (0.5 g/L TiO<sub>2</sub>, UV-A), whereas similar or shorter processing is required when heterogenous photocatalysis is performed using microorganism-specific optimized TiO<sub>2</sub> concentrations and H<sub>2</sub>O<sub>2</sub> supplementation (100 mg/L); higher H<sub>2</sub>O<sub>2</sub> concentrations further enhance the heterogenous photocatalytic inactivation efficiency. Our study provides a template protocol for the design and further application for large-scale photocatalytic approaches to inactivate pathogens in liquid biomedical waste.https://www.mdpi.com/1420-3049/28/3/1199photocatalytic oxidationTiO<sub>2</sub>photo-Fentonmicroorganism inactivation<i>Bacillus stearothermophilus</i> sporesMS2 |
| spellingShingle | Eirini Kanata Ioannis Paspaltsis Sotiris Sotiriadis Chrysanthi Berberidou Sophia Tsoumachidou Dimitra Dafou Konstantinos Xanthopoulos Minas Arsenakis Athanasios Arsenakis Ioannis Poulios Theodoros Sklaviadis Photo-Fenton and TiO<sub>2</sub> Photocatalytic Inactivation of Model Microorganisms under UV-A; Comparative Efficacy and Optimization Molecules photocatalytic oxidation TiO<sub>2</sub> photo-Fenton microorganism inactivation <i>Bacillus stearothermophilus</i> spores MS2 |
| title | Photo-Fenton and TiO<sub>2</sub> Photocatalytic Inactivation of Model Microorganisms under UV-A; Comparative Efficacy and Optimization |
| title_full | Photo-Fenton and TiO<sub>2</sub> Photocatalytic Inactivation of Model Microorganisms under UV-A; Comparative Efficacy and Optimization |
| title_fullStr | Photo-Fenton and TiO<sub>2</sub> Photocatalytic Inactivation of Model Microorganisms under UV-A; Comparative Efficacy and Optimization |
| title_full_unstemmed | Photo-Fenton and TiO<sub>2</sub> Photocatalytic Inactivation of Model Microorganisms under UV-A; Comparative Efficacy and Optimization |
| title_short | Photo-Fenton and TiO<sub>2</sub> Photocatalytic Inactivation of Model Microorganisms under UV-A; Comparative Efficacy and Optimization |
| title_sort | photo fenton and tio sub 2 sub photocatalytic inactivation of model microorganisms under uv a comparative efficacy and optimization |
| topic | photocatalytic oxidation TiO<sub>2</sub> photo-Fenton microorganism inactivation <i>Bacillus stearothermophilus</i> spores MS2 |
| url | https://www.mdpi.com/1420-3049/28/3/1199 |
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