Innate-like T cells in children with sickle cell disease.
BACKGROUND:The implication of lymphocytes in sickle cell disease pathogenesis is supported by a number of recent reports. These studies provided evidence for the activation of invariant natural killer T (iNKT) cells in adult patients, but did not investigate the involvement of other innate-like T ce...
| Main Authors: | , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Public Library of Science (PLoS)
2019-01-01
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| Series: | PLoS ONE |
| Online Access: | https://doi.org/10.1371/journal.pone.0219047 |
| _version_ | 1818986493262168064 |
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| author | Slimane Allali Céline Dietrich François Machavoine Rachel Rignault-Bricard Valentine Brousse Mariane de Montalembert Olivier Hermine Thiago Trovati Maciel Maria Leite-de-Moraes |
| author_facet | Slimane Allali Céline Dietrich François Machavoine Rachel Rignault-Bricard Valentine Brousse Mariane de Montalembert Olivier Hermine Thiago Trovati Maciel Maria Leite-de-Moraes |
| author_sort | Slimane Allali |
| collection | DOAJ |
| description | BACKGROUND:The implication of lymphocytes in sickle cell disease pathogenesis is supported by a number of recent reports. These studies provided evidence for the activation of invariant natural killer T (iNKT) cells in adult patients, but did not investigate the involvement of other innate-like T cell subsets so far. METHODS:Here we present a monocentric prospective observational study evaluating the number and functional properties of both circulating conventional and innate-like T cells, namely iNKT, Mucosal-Associated Invariant T (MAIT) and gammadelta (γδ) T cells in a cohort of 39 children with sickle cell disease. RESULTS:Relative to age-matched healthy controls, we found that patients had a higher frequency of IL-13- and IL-17-producing CD4+ T cells, as well as higher MAIT cell counts with an increased frequency of IL-17-producing MAIT cells. Patients also presented increased Vδ2 γδ T cell counts, especially during vaso-occlusive crisis, and a lower frequency of IFNγ-producing Vδ2 γδ T cells, except during crisis. iNKT cell counts and the frequency of IFNγ-producing iNKT cells were unchanged compared to controls. Our study revealed positive correlations between 1) the frequency of IFNγ-producing CD4+, CD8+ and Vδ2 γδ T cells and the number of hospitalizations for vaso-occlusive crisis in the previous year; 2) the frequency of IFNγ-producing iNKT cells and patients' age and 3) the frequency of IL-17-producing Vδ2 γδ T cells and hemoglobin S level. CONCLUSION:These results strongly suggest a role of innate-like T cells in sickle cell disease pathophysiology, especially that of IL-17-producing MAIT and γδ T cells. |
| first_indexed | 2024-12-20T18:51:40Z |
| format | Article |
| id | doaj.art-a2fff78dcc0d4b788d7f409f8e4b019c |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-12-20T18:51:40Z |
| publishDate | 2019-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-a2fff78dcc0d4b788d7f409f8e4b019c2022-12-21T19:29:36ZengPublic Library of Science (PLoS)PLoS ONE1932-62032019-01-01146e021904710.1371/journal.pone.0219047Innate-like T cells in children with sickle cell disease.Slimane AllaliCéline DietrichFrançois MachavoineRachel Rignault-BricardValentine BrousseMariane de MontalembertOlivier HermineThiago Trovati MacielMaria Leite-de-MoraesBACKGROUND:The implication of lymphocytes in sickle cell disease pathogenesis is supported by a number of recent reports. These studies provided evidence for the activation of invariant natural killer T (iNKT) cells in adult patients, but did not investigate the involvement of other innate-like T cell subsets so far. METHODS:Here we present a monocentric prospective observational study evaluating the number and functional properties of both circulating conventional and innate-like T cells, namely iNKT, Mucosal-Associated Invariant T (MAIT) and gammadelta (γδ) T cells in a cohort of 39 children with sickle cell disease. RESULTS:Relative to age-matched healthy controls, we found that patients had a higher frequency of IL-13- and IL-17-producing CD4+ T cells, as well as higher MAIT cell counts with an increased frequency of IL-17-producing MAIT cells. Patients also presented increased Vδ2 γδ T cell counts, especially during vaso-occlusive crisis, and a lower frequency of IFNγ-producing Vδ2 γδ T cells, except during crisis. iNKT cell counts and the frequency of IFNγ-producing iNKT cells were unchanged compared to controls. Our study revealed positive correlations between 1) the frequency of IFNγ-producing CD4+, CD8+ and Vδ2 γδ T cells and the number of hospitalizations for vaso-occlusive crisis in the previous year; 2) the frequency of IFNγ-producing iNKT cells and patients' age and 3) the frequency of IL-17-producing Vδ2 γδ T cells and hemoglobin S level. CONCLUSION:These results strongly suggest a role of innate-like T cells in sickle cell disease pathophysiology, especially that of IL-17-producing MAIT and γδ T cells.https://doi.org/10.1371/journal.pone.0219047 |
| spellingShingle | Slimane Allali Céline Dietrich François Machavoine Rachel Rignault-Bricard Valentine Brousse Mariane de Montalembert Olivier Hermine Thiago Trovati Maciel Maria Leite-de-Moraes Innate-like T cells in children with sickle cell disease. PLoS ONE |
| title | Innate-like T cells in children with sickle cell disease. |
| title_full | Innate-like T cells in children with sickle cell disease. |
| title_fullStr | Innate-like T cells in children with sickle cell disease. |
| title_full_unstemmed | Innate-like T cells in children with sickle cell disease. |
| title_short | Innate-like T cells in children with sickle cell disease. |
| title_sort | innate like t cells in children with sickle cell disease |
| url | https://doi.org/10.1371/journal.pone.0219047 |
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