The effects of DNA methylation and epigenetic factors on the expression of CD133 in ovarian cancers
<p>Abstract</p> <p>Background</p> <p>To identifying the effects of DNA methylation and epigenetic factors on the expression of CD133, a cancer stem cell marker, in gynecologic cancer cell lines.</p> <p>Methods</p> <p>Ovarian cancer cell lines (OV...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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BMC
2012-10-01
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| Series: | Journal of Ovarian Research |
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| Online Access: | http://www.ovarianresearch.com/content/5/1/28 |
| _version_ | 1797968295805059072 |
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| author | Min Kyung-Jin So Kyeong A Ouh Yung-Taek Hong Jin-Hwa Lee Jae-Kwan |
| author_facet | Min Kyung-Jin So Kyeong A Ouh Yung-Taek Hong Jin-Hwa Lee Jae-Kwan |
| author_sort | Min Kyung-Jin |
| collection | DOAJ |
| description | <p>Abstract</p> <p>Background</p> <p>To identifying the effects of DNA methylation and epigenetic factors on the expression of CD133, a cancer stem cell marker, in gynecologic cancer cell lines.</p> <p>Methods</p> <p>Ovarian cancer cell lines (OVCAR-8 and IGROV-1) and an endometrial cancer cell line (Ishikawa) were treated with 5-aza-2`-deoxycytidine (DAC) or Trichostatin A (TSA). Expression of CD133 was evaluated by quantitative real-time PCR, methylation-specific PCR (MSP), reverse transcription<b>-</b>PCR, western blot, and FACS analysis. All results are representative of three independent experiments.</p> <p>Results</p> <p>CD133 mRNA expression varied among the different cell lines; the weakest expression was observed in OVCAR-8 cells, while it was strongly expressed in Ishikawa cells. The degree of methylation of the CD133 P2 promoter was 61% in OVCAR-8 cells, 53% in IGROV-1 cells, and 43% in Ishikawa cells. CD133 expression was increased at both the mRNA and protein level after DAC treatment. On the contrary, CD133 mRNA expression decreased after TSA treatment decreased in all cell lines except OVCAR-8. In addition, MSP of the CD133 P2 promoter revealed that methylation was reduced after treatment with either DAC or TSA.</p> <p>Conclusions</p> <p>The expression of the CD133 antigen in primary ovarian and endometrial cancer cell lines is regulated by epigenetics, as indicated by its increased expression following DAC treatment and irregular expression pattern followed by TSA treatment. In addition, the expression of CD133 was negatively correlated with the degree of methylation of the CD133 P2 promoter.</p> |
| first_indexed | 2024-04-11T02:44:38Z |
| format | Article |
| id | doaj.art-a302f7f03d594d3b9ac6ee9d3a7a4154 |
| institution | Directory Open Access Journal |
| issn | 1757-2215 |
| language | English |
| last_indexed | 2024-04-11T02:44:38Z |
| publishDate | 2012-10-01 |
| publisher | BMC |
| record_format | Article |
| series | Journal of Ovarian Research |
| spelling | doaj.art-a302f7f03d594d3b9ac6ee9d3a7a41542023-01-02T18:15:32ZengBMCJournal of Ovarian Research1757-22152012-10-01512810.1186/1757-2215-5-28The effects of DNA methylation and epigenetic factors on the expression of CD133 in ovarian cancersMin Kyung-JinSo Kyeong AOuh Yung-TaekHong Jin-HwaLee Jae-Kwan<p>Abstract</p> <p>Background</p> <p>To identifying the effects of DNA methylation and epigenetic factors on the expression of CD133, a cancer stem cell marker, in gynecologic cancer cell lines.</p> <p>Methods</p> <p>Ovarian cancer cell lines (OVCAR-8 and IGROV-1) and an endometrial cancer cell line (Ishikawa) were treated with 5-aza-2`-deoxycytidine (DAC) or Trichostatin A (TSA). Expression of CD133 was evaluated by quantitative real-time PCR, methylation-specific PCR (MSP), reverse transcription<b>-</b>PCR, western blot, and FACS analysis. All results are representative of three independent experiments.</p> <p>Results</p> <p>CD133 mRNA expression varied among the different cell lines; the weakest expression was observed in OVCAR-8 cells, while it was strongly expressed in Ishikawa cells. The degree of methylation of the CD133 P2 promoter was 61% in OVCAR-8 cells, 53% in IGROV-1 cells, and 43% in Ishikawa cells. CD133 expression was increased at both the mRNA and protein level after DAC treatment. On the contrary, CD133 mRNA expression decreased after TSA treatment decreased in all cell lines except OVCAR-8. In addition, MSP of the CD133 P2 promoter revealed that methylation was reduced after treatment with either DAC or TSA.</p> <p>Conclusions</p> <p>The expression of the CD133 antigen in primary ovarian and endometrial cancer cell lines is regulated by epigenetics, as indicated by its increased expression following DAC treatment and irregular expression pattern followed by TSA treatment. In addition, the expression of CD133 was negatively correlated with the degree of methylation of the CD133 P2 promoter.</p>http://www.ovarianresearch.com/content/5/1/28Cancer stem cellCD133Epigenetics5`-aza-2`-deoxycytidineTrichostatin A |
| spellingShingle | Min Kyung-Jin So Kyeong A Ouh Yung-Taek Hong Jin-Hwa Lee Jae-Kwan The effects of DNA methylation and epigenetic factors on the expression of CD133 in ovarian cancers Journal of Ovarian Research Cancer stem cell CD133 Epigenetics 5`-aza-2`-deoxycytidine Trichostatin A |
| title | The effects of DNA methylation and epigenetic factors on the expression of CD133 in ovarian cancers |
| title_full | The effects of DNA methylation and epigenetic factors on the expression of CD133 in ovarian cancers |
| title_fullStr | The effects of DNA methylation and epigenetic factors on the expression of CD133 in ovarian cancers |
| title_full_unstemmed | The effects of DNA methylation and epigenetic factors on the expression of CD133 in ovarian cancers |
| title_short | The effects of DNA methylation and epigenetic factors on the expression of CD133 in ovarian cancers |
| title_sort | effects of dna methylation and epigenetic factors on the expression of cd133 in ovarian cancers |
| topic | Cancer stem cell CD133 Epigenetics 5`-aza-2`-deoxycytidine Trichostatin A |
| url | http://www.ovarianresearch.com/content/5/1/28 |
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