NBS1 plays a synergistic role with telomerase in the maintenance of telomeres in <it>Arabidopsis thaliana</it>
<p>Abstract</p> <p>Background</p> <p>Telomeres, as elaborate nucleo-protein complexes, ensure chromosomal stability. When impaired, the ends of linear chromosomes can be recognised by cellular repair mechanisms as double-strand DNA breaks and can be healed by non-homolo...
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| Format: | Article |
| Language: | English |
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BMC
2012-09-01
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| Series: | BMC Plant Biology |
| Online Access: | http://www.biomedcentral.com/1471-2229/12/167 |
| _version_ | 1818552826094157824 |
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| author | Najdekrova Lucie Siroky Jiri |
| author_facet | Najdekrova Lucie Siroky Jiri |
| author_sort | Najdekrova Lucie |
| collection | DOAJ |
| description | <p>Abstract</p> <p>Background</p> <p>Telomeres, as elaborate nucleo-protein complexes, ensure chromosomal stability. When impaired, the ends of linear chromosomes can be recognised by cellular repair mechanisms as double-strand DNA breaks and can be healed by non-homologous-end-joining activities to produce dicentric chromosomes. During cell divisions, particularly during anaphase, dicentrics can break, thus producing naked chromosome tips susceptible to additional unwanted chromosome fusion. Many telomere-building protein complexes are associated with telomeres to ensure their proper capping function. It has been found however, that a number of repair complexes also contribute to telomere stability.</p> <p>Results</p> <p>We used <it>Arabidopsis thaliana</it> to study the possible functions of the DNA repair subunit, NBS1, in telomere homeostasis using knockout <it>nbs1</it> mutants. The results showed that although NBS1-deficient plants were viable, lacked any sign of developmental aberration and produced fertile seeds through many generations upon self-fertilisation, plants also missing the functional telomerase (double mutants), rapidly, within three generations, displayed severe developmental defects. Cytogenetic inspection of cycling somatic cells revealed a very early onset of massive genome instability. Molecular methods used for examining the length of telomeres in double homozygous mutants detected much faster telomere shortening than in plants deficient in telomerase gene alone.</p> <p>Conclusions</p> <p>Our findings suggest that NBS1 acts in concert with telomerase and plays a profound role in plant telomere renewal.</p> |
| first_indexed | 2024-12-12T09:18:10Z |
| format | Article |
| id | doaj.art-a303a6bbdf22456bb9a99f145119139b |
| institution | Directory Open Access Journal |
| issn | 1471-2229 |
| language | English |
| last_indexed | 2024-12-12T09:18:10Z |
| publishDate | 2012-09-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Plant Biology |
| spelling | doaj.art-a303a6bbdf22456bb9a99f145119139b2022-12-22T00:29:19ZengBMCBMC Plant Biology1471-22292012-09-0112116710.1186/1471-2229-12-167NBS1 plays a synergistic role with telomerase in the maintenance of telomeres in <it>Arabidopsis thaliana</it>Najdekrova LucieSiroky Jiri<p>Abstract</p> <p>Background</p> <p>Telomeres, as elaborate nucleo-protein complexes, ensure chromosomal stability. When impaired, the ends of linear chromosomes can be recognised by cellular repair mechanisms as double-strand DNA breaks and can be healed by non-homologous-end-joining activities to produce dicentric chromosomes. During cell divisions, particularly during anaphase, dicentrics can break, thus producing naked chromosome tips susceptible to additional unwanted chromosome fusion. Many telomere-building protein complexes are associated with telomeres to ensure their proper capping function. It has been found however, that a number of repair complexes also contribute to telomere stability.</p> <p>Results</p> <p>We used <it>Arabidopsis thaliana</it> to study the possible functions of the DNA repair subunit, NBS1, in telomere homeostasis using knockout <it>nbs1</it> mutants. The results showed that although NBS1-deficient plants were viable, lacked any sign of developmental aberration and produced fertile seeds through many generations upon self-fertilisation, plants also missing the functional telomerase (double mutants), rapidly, within three generations, displayed severe developmental defects. Cytogenetic inspection of cycling somatic cells revealed a very early onset of massive genome instability. Molecular methods used for examining the length of telomeres in double homozygous mutants detected much faster telomere shortening than in plants deficient in telomerase gene alone.</p> <p>Conclusions</p> <p>Our findings suggest that NBS1 acts in concert with telomerase and plays a profound role in plant telomere renewal.</p>http://www.biomedcentral.com/1471-2229/12/167 |
| spellingShingle | Najdekrova Lucie Siroky Jiri NBS1 plays a synergistic role with telomerase in the maintenance of telomeres in <it>Arabidopsis thaliana</it> BMC Plant Biology |
| title | NBS1 plays a synergistic role with telomerase in the maintenance of telomeres in <it>Arabidopsis thaliana</it> |
| title_full | NBS1 plays a synergistic role with telomerase in the maintenance of telomeres in <it>Arabidopsis thaliana</it> |
| title_fullStr | NBS1 plays a synergistic role with telomerase in the maintenance of telomeres in <it>Arabidopsis thaliana</it> |
| title_full_unstemmed | NBS1 plays a synergistic role with telomerase in the maintenance of telomeres in <it>Arabidopsis thaliana</it> |
| title_short | NBS1 plays a synergistic role with telomerase in the maintenance of telomeres in <it>Arabidopsis thaliana</it> |
| title_sort | nbs1 plays a synergistic role with telomerase in the maintenance of telomeres in it arabidopsis thaliana it |
| url | http://www.biomedcentral.com/1471-2229/12/167 |
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