Hemagglutinin expressed by yeast reshapes immune microenvironment and gut microbiota to trigger diverse anti-infection response in infected birds
IntroductionThe H5N8 influenza virus is a highly pathogenic pathogen for poultry and human. Vaccination is the most effective method to control the spread of the virus right now. The traditional inactivated vaccine, though well developed and used widely, is laborious during application and more inte...
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Frontiers Media S.A.
2023-04-01
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| Series: | Frontiers in Immunology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fimmu.2023.1125190/full |
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| author | Ruyu Xie Huixia Zhang Han Zhang Changyan Li Daqing Cui Shujun Li Zexing Li Hualei Liu Jinhai Huang |
| author_facet | Ruyu Xie Huixia Zhang Han Zhang Changyan Li Daqing Cui Shujun Li Zexing Li Hualei Liu Jinhai Huang |
| author_sort | Ruyu Xie |
| collection | DOAJ |
| description | IntroductionThe H5N8 influenza virus is a highly pathogenic pathogen for poultry and human. Vaccination is the most effective method to control the spread of the virus right now. The traditional inactivated vaccine, though well developed and used widely, is laborious during application and more interests are stimulated in developing alternative approaches.MethodsIn this study, we developed three hemagglutinin (HA) gene-based yeast vaccine. In order to explore the protective efficacy of the vaccines, the gene expression level in the bursa of Fabricius and the structure of intestinal microflora in immunized animals were analyzed by RNA seq and 16SrRNA sequencing, and the regulatory mechanism of yeast vaccine was evaluated.ResultsAll of these vaccines elicited the humoral immunity, inhibited viral load in the chicken tissues, and provided partial protective efficacy due to the high dose of the H5N8 virus. Molecular mechanism studies suggested that, compared to the traditional inactivated vaccine, our engineered yeast vaccine reshaped the immune cell microenvironment in bursa of Fabricius to promote the defense and immune responses. Analysis of gut microbiota further suggested that oral administration of engineered ST1814G/H5HA yeast vaccine increased the diversity of gut microbiota and the increasement of Reuteri and Muciniphila might benefit the recovery from influenza virus infection. These results provide strong evidence for further clinical use of these engineered yeast vaccine in poultry. |
| first_indexed | 2024-04-09T17:31:43Z |
| format | Article |
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| issn | 1664-3224 |
| language | English |
| last_indexed | 2024-04-09T17:31:43Z |
| publishDate | 2023-04-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Immunology |
| spelling | doaj.art-a304c50727ba4694971e8cdfff53c4502023-04-18T04:49:50ZengFrontiers Media S.A.Frontiers in Immunology1664-32242023-04-011410.3389/fimmu.2023.11251901125190Hemagglutinin expressed by yeast reshapes immune microenvironment and gut microbiota to trigger diverse anti-infection response in infected birdsRuyu Xie0Huixia Zhang1Han Zhang2Changyan Li3Daqing Cui4Shujun Li5Zexing Li6Hualei Liu7Jinhai Huang8School of Life Science, Tianjin University, Tianjin, ChinaSchool of Life Science, Tianjin University, Tianjin, ChinaSchool of Life Science, Tianjin University, Tianjin, ChinaSchool of Life Science, Tianjin University, Tianjin, ChinaSchool of Life Science, Tianjin University, Tianjin, ChinaSchool of Life Science, Tianjin University, Tianjin, ChinaSchool of Life Science, Tianjin University, Tianjin, ChinaChina Animal Health and Epidemiology Center, Qingdao, Shandong, ChinaSchool of Life Science, Tianjin University, Tianjin, ChinaIntroductionThe H5N8 influenza virus is a highly pathogenic pathogen for poultry and human. Vaccination is the most effective method to control the spread of the virus right now. The traditional inactivated vaccine, though well developed and used widely, is laborious during application and more interests are stimulated in developing alternative approaches.MethodsIn this study, we developed three hemagglutinin (HA) gene-based yeast vaccine. In order to explore the protective efficacy of the vaccines, the gene expression level in the bursa of Fabricius and the structure of intestinal microflora in immunized animals were analyzed by RNA seq and 16SrRNA sequencing, and the regulatory mechanism of yeast vaccine was evaluated.ResultsAll of these vaccines elicited the humoral immunity, inhibited viral load in the chicken tissues, and provided partial protective efficacy due to the high dose of the H5N8 virus. Molecular mechanism studies suggested that, compared to the traditional inactivated vaccine, our engineered yeast vaccine reshaped the immune cell microenvironment in bursa of Fabricius to promote the defense and immune responses. Analysis of gut microbiota further suggested that oral administration of engineered ST1814G/H5HA yeast vaccine increased the diversity of gut microbiota and the increasement of Reuteri and Muciniphila might benefit the recovery from influenza virus infection. These results provide strong evidence for further clinical use of these engineered yeast vaccine in poultry. https://www.frontiersin.org/articles/10.3389/fimmu.2023.1125190/fullinfluenza A virusH5N8Saccharomyces cerevisiaevaccinehemagglutinin (HA) |
| spellingShingle | Ruyu Xie Huixia Zhang Han Zhang Changyan Li Daqing Cui Shujun Li Zexing Li Hualei Liu Jinhai Huang Hemagglutinin expressed by yeast reshapes immune microenvironment and gut microbiota to trigger diverse anti-infection response in infected birds Frontiers in Immunology influenza A virus H5N8 Saccharomyces cerevisiae vaccine hemagglutinin (HA) |
| title | Hemagglutinin expressed by yeast reshapes immune microenvironment and gut microbiota to trigger diverse anti-infection response in infected birds |
| title_full | Hemagglutinin expressed by yeast reshapes immune microenvironment and gut microbiota to trigger diverse anti-infection response in infected birds |
| title_fullStr | Hemagglutinin expressed by yeast reshapes immune microenvironment and gut microbiota to trigger diverse anti-infection response in infected birds |
| title_full_unstemmed | Hemagglutinin expressed by yeast reshapes immune microenvironment and gut microbiota to trigger diverse anti-infection response in infected birds |
| title_short | Hemagglutinin expressed by yeast reshapes immune microenvironment and gut microbiota to trigger diverse anti-infection response in infected birds |
| title_sort | hemagglutinin expressed by yeast reshapes immune microenvironment and gut microbiota to trigger diverse anti infection response in infected birds |
| topic | influenza A virus H5N8 Saccharomyces cerevisiae vaccine hemagglutinin (HA) |
| url | https://www.frontiersin.org/articles/10.3389/fimmu.2023.1125190/full |
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