Sequencing and analysis of the complete mitochondrial genome of Rugosa emeljanovi (Anura: Ranidae)

In this study, the complete mitogenome sequence of Rugosa emeljanovi (Anura: Ranidae) is first determined using long PCR. It is a circular molecule of 17,733 bp in length (GenBank accession no. KU641020). Similar to the typical mtDNA of amphibians, the complete mtDNA sequence of R. emeljanovi contai...

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Main Authors: Wanli Liu, Jiuchen Tao, Heng Wang, Wenge Zhao, Peng Liu
Format: Article
Language:English
Published: Taylor & Francis Group 2017-12-01
Series:Mitochondrial DNA. Part B. Resources
Subjects:
Online Access:http://dx.doi.org/10.1080/23802359.2017.1347841
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author Wanli Liu
Jiuchen Tao
Heng Wang
Wenge Zhao
Peng Liu
author_facet Wanli Liu
Jiuchen Tao
Heng Wang
Wenge Zhao
Peng Liu
author_sort Wanli Liu
collection DOAJ
description In this study, the complete mitogenome sequence of Rugosa emeljanovi (Anura: Ranidae) is first determined using long PCR. It is a circular molecule of 17,733 bp in length (GenBank accession no. KU641020). Similar to the typical mtDNA of amphibians, the complete mtDNA sequence of R. emeljanovi contained 2 rRNA genes (12S rRNA and 16S rRNA), 22 tRNA genes, 13 protein-coding genes (PCGs), and a control region (D-loop). The nucleotide composition was 28.1% A, 26.8% C, 14.8% G, and 30.3% T. Mitochondrial genome analyses based on the NJ method yield phylogenetic trees, including the 13 already reported Ranidae family frogs. The molecular data presented here provide a useful tool for systematic analyses of genus Rugosa and Glandirana.
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spelling doaj.art-a30605544c6b44fba73f93900937ad442023-11-02T15:42:56ZengTaylor & Francis GroupMitochondrial DNA. Part B. Resources2380-23592017-12-012238338410.1080/23802359.2017.13478411347841Sequencing and analysis of the complete mitochondrial genome of Rugosa emeljanovi (Anura: Ranidae)Wanli Liu0Jiuchen Tao1Heng Wang2Wenge Zhao3Peng Liu4Harbin Normal UniversityHarbin Normal UniversityHarbin Normal UniversityHarbin Normal UniversityHarbin Normal UniversityIn this study, the complete mitogenome sequence of Rugosa emeljanovi (Anura: Ranidae) is first determined using long PCR. It is a circular molecule of 17,733 bp in length (GenBank accession no. KU641020). Similar to the typical mtDNA of amphibians, the complete mtDNA sequence of R. emeljanovi contained 2 rRNA genes (12S rRNA and 16S rRNA), 22 tRNA genes, 13 protein-coding genes (PCGs), and a control region (D-loop). The nucleotide composition was 28.1% A, 26.8% C, 14.8% G, and 30.3% T. Mitochondrial genome analyses based on the NJ method yield phylogenetic trees, including the 13 already reported Ranidae family frogs. The molecular data presented here provide a useful tool for systematic analyses of genus Rugosa and Glandirana.http://dx.doi.org/10.1080/23802359.2017.1347841ranidaemitogenomerugosa emeljanoviphylogenetic tree
spellingShingle Wanli Liu
Jiuchen Tao
Heng Wang
Wenge Zhao
Peng Liu
Sequencing and analysis of the complete mitochondrial genome of Rugosa emeljanovi (Anura: Ranidae)
Mitochondrial DNA. Part B. Resources
ranidae
mitogenome
rugosa emeljanovi
phylogenetic tree
title Sequencing and analysis of the complete mitochondrial genome of Rugosa emeljanovi (Anura: Ranidae)
title_full Sequencing and analysis of the complete mitochondrial genome of Rugosa emeljanovi (Anura: Ranidae)
title_fullStr Sequencing and analysis of the complete mitochondrial genome of Rugosa emeljanovi (Anura: Ranidae)
title_full_unstemmed Sequencing and analysis of the complete mitochondrial genome of Rugosa emeljanovi (Anura: Ranidae)
title_short Sequencing and analysis of the complete mitochondrial genome of Rugosa emeljanovi (Anura: Ranidae)
title_sort sequencing and analysis of the complete mitochondrial genome of rugosa emeljanovi anura ranidae
topic ranidae
mitogenome
rugosa emeljanovi
phylogenetic tree
url http://dx.doi.org/10.1080/23802359.2017.1347841
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