N-Glycosylation Alteration of Serum and Salivary Immunoglobulin A Is a Possible Biomarker in Oral Mucositis
Background: Oral and enteral mucositis due to high-dose cytostatic treatment administered during autologous and allogeneic stem-cell transplantation increases mortality. Salivary secretory immunoglobulin A (sIgA) is a basic pillar of local immunity in the first line of defense. Altered salivary sial...
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MDPI AG
2020-06-01
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| Series: | Journal of Clinical Medicine |
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| Online Access: | https://www.mdpi.com/2077-0383/9/6/1747 |
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| author | Enikő Gebri Zsuzsanna Kovács Brigitta Mészáros Ferenc Tóth Ádám Simon Hajnalka Jankovics Ferenc Vonderviszt Attila Kiss András Guttman Tibor Hortobágyi |
| author_facet | Enikő Gebri Zsuzsanna Kovács Brigitta Mészáros Ferenc Tóth Ádám Simon Hajnalka Jankovics Ferenc Vonderviszt Attila Kiss András Guttman Tibor Hortobágyi |
| author_sort | Enikő Gebri |
| collection | DOAJ |
| description | Background: Oral and enteral mucositis due to high-dose cytostatic treatment administered during autologous and allogeneic stem-cell transplantation increases mortality. Salivary secretory immunoglobulin A (sIgA) is a basic pillar of local immunity in the first line of defense. Altered salivary sialoglycoprotein carbohydrates are important in the pathologies in the oral cavity including inflammation, infection and neoplasia. Therefore, we assessed whether changes in the salivary and serum IgA glycosylation correlated with development and severity of oral mucositis. Methods: Using capillary electrophoresis, comparative analysis of serum and salivary IgA total N-glycans was conducted in 8 patients with autologous peripheral stem-cell transplantation (APSCT) at four different stages of transplantation (day −3/−7, 0, +7, +14) and in 10 healthy controls. Results: Fourteen out of the 31 structures identified in serum and 6 out of 38 in saliva showed significant changes upon transplantation compared with the control group. Only serum core fucosylated, sialylated bisecting biantennary glycan (FA2BG2S2) showed significant differences between any two stages of transplantation (day −3/−7 and day +14; <i>p</i> = 0.0279). Conclusion: Our results suggest that changes in the serum IgA total N-glycan profile could serve as a disease-specific biomarker in patients undergoing APSCT, while analysis of salivary IgA N-glycan reflects the effect of APSCT on local immunity. |
| first_indexed | 2024-03-10T19:22:01Z |
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| id | doaj.art-a307021fa9254796b1a12574207a9145 |
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| issn | 2077-0383 |
| language | English |
| last_indexed | 2024-03-10T19:22:01Z |
| publishDate | 2020-06-01 |
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| series | Journal of Clinical Medicine |
| spelling | doaj.art-a307021fa9254796b1a12574207a91452023-11-20T02:55:35ZengMDPI AGJournal of Clinical Medicine2077-03832020-06-0196174710.3390/jcm9061747N-Glycosylation Alteration of Serum and Salivary Immunoglobulin A Is a Possible Biomarker in Oral MucositisEnikő Gebri0Zsuzsanna Kovács1Brigitta Mészáros2Ferenc Tóth3Ádám Simon4Hajnalka Jankovics5Ferenc Vonderviszt6Attila Kiss7András Guttman8Tibor Hortobágyi9Department of Dentoalveolar Surgery and Dental Outpatient Care, Faculty of Dentistry, University of Debrecen, Nagyerdei krt. 98., H-4032 Debrecen, HungaryHorváth Csaba Laboratory of Bioseparation Sciences, Research Center for Molecular Medicine, Doctoral School of Molecular Medicine, Faculty of Medicine, University of Debrecen, Nagyerdei krt. 98., H-4032 Debrecen, HungaryHorváth Csaba Laboratory of Bioseparation Sciences, Research Center for Molecular Medicine, Doctoral School of Molecular Medicine, Faculty of Medicine, University of Debrecen, Nagyerdei krt. 98., H-4032 Debrecen, HungaryDepartment of Biomaterials and Prosthetic Dentistry, Faculty of Dentistry, University of Debrecen, Nagyerdei krt. 98., H-4032 Debrecen, HungaryHorváth Csaba Laboratory of Bioseparation Sciences, Research Center for Molecular Medicine, Doctoral School of Molecular Medicine, Faculty of Medicine, University of Debrecen, Nagyerdei krt. 98., H-4032 Debrecen, HungaryResearch Institute of Biomolecular and Chemical Engineering, University of Pannonia, Egyetem u 10., H-8200 Veszprém, HungaryResearch Institute of Biomolecular and Chemical Engineering, University of Pannonia, Egyetem u 10., H-8200 Veszprém, HungaryDepartment of Hematopoietic Transplantation Centre, Faculty of Medicine, University of Debrecen, Nagyerdei krt. 98., H-4032 Debrecen, HungaryHorváth Csaba Laboratory of Bioseparation Sciences, Research Center for Molecular Medicine, Doctoral School of Molecular Medicine, Faculty of Medicine, University of Debrecen, Nagyerdei krt. 98., H-4032 Debrecen, HungaryInstitute of Pathology, Faculty of Medicine, University of Szeged, Állomás utca 1., H-6725 Szeged, HungaryBackground: Oral and enteral mucositis due to high-dose cytostatic treatment administered during autologous and allogeneic stem-cell transplantation increases mortality. Salivary secretory immunoglobulin A (sIgA) is a basic pillar of local immunity in the first line of defense. Altered salivary sialoglycoprotein carbohydrates are important in the pathologies in the oral cavity including inflammation, infection and neoplasia. Therefore, we assessed whether changes in the salivary and serum IgA glycosylation correlated with development and severity of oral mucositis. Methods: Using capillary electrophoresis, comparative analysis of serum and salivary IgA total N-glycans was conducted in 8 patients with autologous peripheral stem-cell transplantation (APSCT) at four different stages of transplantation (day −3/−7, 0, +7, +14) and in 10 healthy controls. Results: Fourteen out of the 31 structures identified in serum and 6 out of 38 in saliva showed significant changes upon transplantation compared with the control group. Only serum core fucosylated, sialylated bisecting biantennary glycan (FA2BG2S2) showed significant differences between any two stages of transplantation (day −3/−7 and day +14; <i>p</i> = 0.0279). Conclusion: Our results suggest that changes in the serum IgA total N-glycan profile could serve as a disease-specific biomarker in patients undergoing APSCT, while analysis of salivary IgA N-glycan reflects the effect of APSCT on local immunity.https://www.mdpi.com/2077-0383/9/6/1747autologous peripheral stem-cell transplantation (APSCT)glycomicsglycoproteinimmunoglobulinsIgAoral immunity |
| spellingShingle | Enikő Gebri Zsuzsanna Kovács Brigitta Mészáros Ferenc Tóth Ádám Simon Hajnalka Jankovics Ferenc Vonderviszt Attila Kiss András Guttman Tibor Hortobágyi N-Glycosylation Alteration of Serum and Salivary Immunoglobulin A Is a Possible Biomarker in Oral Mucositis Journal of Clinical Medicine autologous peripheral stem-cell transplantation (APSCT) glycomics glycoprotein immunoglobulins IgA oral immunity |
| title | N-Glycosylation Alteration of Serum and Salivary Immunoglobulin A Is a Possible Biomarker in Oral Mucositis |
| title_full | N-Glycosylation Alteration of Serum and Salivary Immunoglobulin A Is a Possible Biomarker in Oral Mucositis |
| title_fullStr | N-Glycosylation Alteration of Serum and Salivary Immunoglobulin A Is a Possible Biomarker in Oral Mucositis |
| title_full_unstemmed | N-Glycosylation Alteration of Serum and Salivary Immunoglobulin A Is a Possible Biomarker in Oral Mucositis |
| title_short | N-Glycosylation Alteration of Serum and Salivary Immunoglobulin A Is a Possible Biomarker in Oral Mucositis |
| title_sort | n glycosylation alteration of serum and salivary immunoglobulin a is a possible biomarker in oral mucositis |
| topic | autologous peripheral stem-cell transplantation (APSCT) glycomics glycoprotein immunoglobulins IgA oral immunity |
| url | https://www.mdpi.com/2077-0383/9/6/1747 |
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