Stearoyl CoA desaturase and fatty acid synthase gene polymorphisms and milk fatty acid composition in Chilean Black Friesian cows
Background: in recent years, market attention has increasingly focused on improving the quality of dairy products. Therefore, animal selection for healthier milk fatty acid composition are therefore of interest. Objective: the aim of this study was to identify whether two polymorphisms (c.878T>C...
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| Format: | Article |
| Language: | English |
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Universidad de Antioquia
2013-12-01
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| Series: | Revista Colombiana de Ciencias Pecuarias |
| Subjects: | |
| Online Access: | http://www.scielo.org.co/scielo.php?script=sci_arttext&pid=S0120-06902013000400004&lng=en&tlng=en |
| _version_ | 1819206777975078912 |
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| author | Karla B Inostroza Erick S Scheuermann Néstor A Sepúlveda |
| author_facet | Karla B Inostroza Erick S Scheuermann Néstor A Sepúlveda |
| author_sort | Karla B Inostroza |
| collection | DOAJ |
| description | Background: in recent years, market attention has increasingly focused on improving the quality of dairy products. Therefore, animal selection for healthier milk fatty acid composition are therefore of interest. Objective: the aim of this study was to identify whether two polymorphisms (c.878T>C in the SCD gene and g.17924A>G in the FASN gene) are related with the fatty acid composition of milk. Methods: individual milk samples were collected from 50 Chilean Black Friesian cows. Fatty acids (FA) from each milk sample were extracted, analyzed, and quantified (mg FA/g of milk) by gas chromatography. The genotype of each sample was also determined using PCR-RFLP (Polymerase Chain Reaction - Restriction Fragment Length Polymorphism). Results: milk from cows with a c.878CC genotype in the SCD gene exhibited a greater content of C14:1, C17:1, C18:1n9t, C18:1n9c, and total monounsaturated fatty acids (MUFA), as well as a higher 14:1/14 ratio than other SCD genotypes. In addition, considering the importance of the predominant isomer of conjugated linoleic acid for human diet, we identified a higher content of cis-9, trans-11 conjugated linoleic acid in milk samples from animals that had the c.878CC genotype. For the FASN gene, cows with the g.17924GG genotype presented high contents of C14:0, C16:0, C18:0, C18:1n9t, C18:1n9c, and MUFA. Conclusion: therefore, these polymorphisms could be useful genetic markers for studying the fatty acid composition of dairy milk. |
| first_indexed | 2024-12-23T05:13:00Z |
| format | Article |
| id | doaj.art-a307eadb10534ec991297959ba9c0db5 |
| institution | Directory Open Access Journal |
| issn | 0120-0690 |
| language | English |
| last_indexed | 2024-12-23T05:13:00Z |
| publishDate | 2013-12-01 |
| publisher | Universidad de Antioquia |
| record_format | Article |
| series | Revista Colombiana de Ciencias Pecuarias |
| spelling | doaj.art-a307eadb10534ec991297959ba9c0db52022-12-21T17:58:55ZengUniversidad de AntioquiaRevista Colombiana de Ciencias Pecuarias0120-06902013-12-01264263269S0120-06902013000400004Stearoyl CoA desaturase and fatty acid synthase gene polymorphisms and milk fatty acid composition in Chilean Black Friesian cowsKarla B Inostroza0Erick S Scheuermann1Néstor A Sepúlveda2Universidad de La FronteraUniversidad de La FronteraUniversidad de La FronteraBackground: in recent years, market attention has increasingly focused on improving the quality of dairy products. Therefore, animal selection for healthier milk fatty acid composition are therefore of interest. Objective: the aim of this study was to identify whether two polymorphisms (c.878T>C in the SCD gene and g.17924A>G in the FASN gene) are related with the fatty acid composition of milk. Methods: individual milk samples were collected from 50 Chilean Black Friesian cows. Fatty acids (FA) from each milk sample were extracted, analyzed, and quantified (mg FA/g of milk) by gas chromatography. The genotype of each sample was also determined using PCR-RFLP (Polymerase Chain Reaction - Restriction Fragment Length Polymorphism). Results: milk from cows with a c.878CC genotype in the SCD gene exhibited a greater content of C14:1, C17:1, C18:1n9t, C18:1n9c, and total monounsaturated fatty acids (MUFA), as well as a higher 14:1/14 ratio than other SCD genotypes. In addition, considering the importance of the predominant isomer of conjugated linoleic acid for human diet, we identified a higher content of cis-9, trans-11 conjugated linoleic acid in milk samples from animals that had the c.878CC genotype. For the FASN gene, cows with the g.17924GG genotype presented high contents of C14:0, C16:0, C18:0, C18:1n9t, C18:1n9c, and MUFA. Conclusion: therefore, these polymorphisms could be useful genetic markers for studying the fatty acid composition of dairy milk.http://www.scielo.org.co/scielo.php?script=sci_arttext&pid=S0120-06902013000400004&lng=en&tlng=enácido linoleico conjugadomonoinsaturadosSNP |
| spellingShingle | Karla B Inostroza Erick S Scheuermann Néstor A Sepúlveda Stearoyl CoA desaturase and fatty acid synthase gene polymorphisms and milk fatty acid composition in Chilean Black Friesian cows Revista Colombiana de Ciencias Pecuarias ácido linoleico conjugado monoinsaturados SNP |
| title | Stearoyl CoA desaturase and fatty acid synthase gene polymorphisms and milk fatty acid composition in Chilean Black Friesian cows |
| title_full | Stearoyl CoA desaturase and fatty acid synthase gene polymorphisms and milk fatty acid composition in Chilean Black Friesian cows |
| title_fullStr | Stearoyl CoA desaturase and fatty acid synthase gene polymorphisms and milk fatty acid composition in Chilean Black Friesian cows |
| title_full_unstemmed | Stearoyl CoA desaturase and fatty acid synthase gene polymorphisms and milk fatty acid composition in Chilean Black Friesian cows |
| title_short | Stearoyl CoA desaturase and fatty acid synthase gene polymorphisms and milk fatty acid composition in Chilean Black Friesian cows |
| title_sort | stearoyl coa desaturase and fatty acid synthase gene polymorphisms and milk fatty acid composition in chilean black friesian cows |
| topic | ácido linoleico conjugado monoinsaturados SNP |
| url | http://www.scielo.org.co/scielo.php?script=sci_arttext&pid=S0120-06902013000400004&lng=en&tlng=en |
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