In vitro Antibiotic and Modulatory Activity of <i>Mesosphaerum suaveolens</i> (L.) Kuntze against <i>Candida</i> strains
The emergence of fungal resistance to commercial drugs has been a major problem for the WHO. In this context, research with natural products is promising in the discovery of new active substances. Thus, this work evaluated the antifungal effect of a medicinal plant (i.e., <i>Mesosphaerum suave...
| Main Authors: | , , , , , , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2020-01-01
|
| Series: | Antibiotics |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2079-6382/9/2/46 |
| _version_ | 1818996028092710912 |
|---|---|
| author | Adrielle Rodrigues Costa José Weverton Almeida Bezerra Rafael Pereira da Cruz Maria Audilene de Freitas Viviane Bezerra da Silva João Cruz Neto Antonia Thassya Lucas dos Santos Maria Flaviana Bezerra Morais Braga Leomara Andrade da Silva Maria Ivaneide Rocha Jean Paul Kamdem Marcello Iriti Sara Vitalini Antonia Eliene Duarte Luiz Marivando Barros |
| author_facet | Adrielle Rodrigues Costa José Weverton Almeida Bezerra Rafael Pereira da Cruz Maria Audilene de Freitas Viviane Bezerra da Silva João Cruz Neto Antonia Thassya Lucas dos Santos Maria Flaviana Bezerra Morais Braga Leomara Andrade da Silva Maria Ivaneide Rocha Jean Paul Kamdem Marcello Iriti Sara Vitalini Antonia Eliene Duarte Luiz Marivando Barros |
| author_sort | Adrielle Rodrigues Costa |
| collection | DOAJ |
| description | The emergence of fungal resistance to commercial drugs has been a major problem for the WHO. In this context, research with natural products is promising in the discovery of new active substances. Thus, this work evaluated the antifungal effect of a medicinal plant (i.e., <i>Mesosphaerum suaveolens</i>) against strains of the genus <i>Candida</i>, tested the combined effect with the drug fluconazole, and, finally, determined the phenolic constituents present in the species. Initially, aqueous extracts of leaves (AELMs) and aerial parts (AEAPMs) of the species were prepared. For microbiological assays, the minimum fungicidal concentration was determined by broth microdilution, and the combined effect of fluconazole extracts were verified by sub-inhibitory microdilution concentrations (CFM/8) followed by spectrophotometric readings which were used to determine the IC<sub>50</sub>. HPLC detected the presence of flavonoids and phenolic acids, detecting eight compounds present in the samples of which caffeic acid and quercetin were major components. The AELMs modulated fluconazole activity since it decreased fluconazole’s IC<sub>50</sub> from 7.8 µg/mL to an IC<sub>50</sub> of 4.7 µg/mL (CA LM 77) and from 28.8 µg/mL to 18.26 µg/mL (CA INCQS 40006) for the <i>C. albicans</i> strains. The AEAPMs were able to potentiate the effect of fluconazole more effectively than the AELMs. Such an effect was significant for the 16 µg/mL concentration for CA LM 77 and 32 µg/mL for CA INCQS 40006. The AEAPMs as well as the AELMs presented clinically relevant activities for <i>C. tropicalis</i> strains. For the <i>C. tropicalis</i> LM 23 strain, the AEPMs obtained an IC<sub>50</sub> of 25 µg/mL and the AELMs an IC<sub>50</sub> of 359.9 µg/mL. |
| first_indexed | 2024-12-20T21:23:13Z |
| format | Article |
| id | doaj.art-a30ae0a4333140a4a76403a1af14f39b |
| institution | Directory Open Access Journal |
| issn | 2079-6382 |
| language | English |
| last_indexed | 2024-12-20T21:23:13Z |
| publishDate | 2020-01-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Antibiotics |
| spelling | doaj.art-a30ae0a4333140a4a76403a1af14f39b2022-12-21T19:26:14ZengMDPI AGAntibiotics2079-63822020-01-01924610.3390/antibiotics9020046antibiotics9020046In vitro Antibiotic and Modulatory Activity of <i>Mesosphaerum suaveolens</i> (L.) Kuntze against <i>Candida</i> strainsAdrielle Rodrigues Costa0José Weverton Almeida Bezerra1Rafael Pereira da Cruz2Maria Audilene de Freitas3Viviane Bezerra da Silva4João Cruz Neto5Antonia Thassya Lucas dos Santos6Maria Flaviana Bezerra Morais Braga7Leomara Andrade da Silva8Maria Ivaneide Rocha9Jean Paul Kamdem10Marcello Iriti11Sara Vitalini12Antonia Eliene Duarte13Luiz Marivando Barros14Postgraduate Program in Molecular Bioprospecting, Regional University of Cariri (URCA), Crato 63122-290, CE, BrazilPostgraduate Program of Plant Biology, Federal University of Pernambuco, Recife 50670-901, BrazilLaboratory of Applied Mycology of Cariri, Regional University of Cariri (URCA), Crato 63122-290, CE, BrazilLaboratory of Applied Mycology of Cariri, Regional University of Cariri (URCA), Crato 63122-290, CE, BrazilPostgraduate Program in Molecular Bioprospecting, Regional University of Cariri (URCA), Crato 63122-290, CE, BrazilNursing Course at the Regional University of Cariri (URCA), Crato-CE-BrazilLaboratory of Applied Mycology of Cariri, Regional University of Cariri (URCA), Crato 63122-290, CE, BrazilLaboratory of Applied Mycology of Cariri, Regional University of Cariri (URCA), Crato 63122-290, CE, BrazilPostgraduate Program in Botany – National Amazon Research Institute (INPA), Manaus 69067-375, AM, BrazilBiology and Toxicology Laboratory, University of Regional Cariri (URCA), Crato 63122-290, CE, BrazilBiology and Toxicology Laboratory, University of Regional Cariri (URCA), Crato 63122-290, CE, BrazilDepartment of Agricultural and Environmental Sciences, Milan State University, 20133 Milan, ItalyDepartment of Agricultural and Environmental Sciences, Milan State University, 20133 Milan, ItalyBiology and Toxicology Laboratory, University of Regional Cariri (URCA), Crato 63122-290, CE, BrazilVegetable Ecophysiology Laboratory, Regional University of Cariri, Crato 63122-290, CE, BrazilThe emergence of fungal resistance to commercial drugs has been a major problem for the WHO. In this context, research with natural products is promising in the discovery of new active substances. Thus, this work evaluated the antifungal effect of a medicinal plant (i.e., <i>Mesosphaerum suaveolens</i>) against strains of the genus <i>Candida</i>, tested the combined effect with the drug fluconazole, and, finally, determined the phenolic constituents present in the species. Initially, aqueous extracts of leaves (AELMs) and aerial parts (AEAPMs) of the species were prepared. For microbiological assays, the minimum fungicidal concentration was determined by broth microdilution, and the combined effect of fluconazole extracts were verified by sub-inhibitory microdilution concentrations (CFM/8) followed by spectrophotometric readings which were used to determine the IC<sub>50</sub>. HPLC detected the presence of flavonoids and phenolic acids, detecting eight compounds present in the samples of which caffeic acid and quercetin were major components. The AELMs modulated fluconazole activity since it decreased fluconazole’s IC<sub>50</sub> from 7.8 µg/mL to an IC<sub>50</sub> of 4.7 µg/mL (CA LM 77) and from 28.8 µg/mL to 18.26 µg/mL (CA INCQS 40006) for the <i>C. albicans</i> strains. The AEAPMs were able to potentiate the effect of fluconazole more effectively than the AELMs. Such an effect was significant for the 16 µg/mL concentration for CA LM 77 and 32 µg/mL for CA INCQS 40006. The AEAPMs as well as the AELMs presented clinically relevant activities for <i>C. tropicalis</i> strains. For the <i>C. tropicalis</i> LM 23 strain, the AEPMs obtained an IC<sub>50</sub> of 25 µg/mL and the AELMs an IC<sub>50</sub> of 359.9 µg/mL.https://www.mdpi.com/2079-6382/9/2/46<i>hyptis suaveolens</i><i>candida</i>hplc-dadbamburralphychochemistry |
| spellingShingle | Adrielle Rodrigues Costa José Weverton Almeida Bezerra Rafael Pereira da Cruz Maria Audilene de Freitas Viviane Bezerra da Silva João Cruz Neto Antonia Thassya Lucas dos Santos Maria Flaviana Bezerra Morais Braga Leomara Andrade da Silva Maria Ivaneide Rocha Jean Paul Kamdem Marcello Iriti Sara Vitalini Antonia Eliene Duarte Luiz Marivando Barros In vitro Antibiotic and Modulatory Activity of <i>Mesosphaerum suaveolens</i> (L.) Kuntze against <i>Candida</i> strains Antibiotics <i>hyptis suaveolens</i> <i>candida</i> hplc-dad bamburral phychochemistry |
| title | In vitro Antibiotic and Modulatory Activity of <i>Mesosphaerum suaveolens</i> (L.) Kuntze against <i>Candida</i> strains |
| title_full | In vitro Antibiotic and Modulatory Activity of <i>Mesosphaerum suaveolens</i> (L.) Kuntze against <i>Candida</i> strains |
| title_fullStr | In vitro Antibiotic and Modulatory Activity of <i>Mesosphaerum suaveolens</i> (L.) Kuntze against <i>Candida</i> strains |
| title_full_unstemmed | In vitro Antibiotic and Modulatory Activity of <i>Mesosphaerum suaveolens</i> (L.) Kuntze against <i>Candida</i> strains |
| title_short | In vitro Antibiotic and Modulatory Activity of <i>Mesosphaerum suaveolens</i> (L.) Kuntze against <i>Candida</i> strains |
| title_sort | in vitro antibiotic and modulatory activity of i mesosphaerum suaveolens i l kuntze against i candida i strains |
| topic | <i>hyptis suaveolens</i> <i>candida</i> hplc-dad bamburral phychochemistry |
| url | https://www.mdpi.com/2079-6382/9/2/46 |
| work_keys_str_mv | AT adriellerodriguescosta invitroantibioticandmodulatoryactivityofimesosphaerumsuaveolensilkuntzeagainsticandidaistrains AT josewevertonalmeidabezerra invitroantibioticandmodulatoryactivityofimesosphaerumsuaveolensilkuntzeagainsticandidaistrains AT rafaelpereiradacruz invitroantibioticandmodulatoryactivityofimesosphaerumsuaveolensilkuntzeagainsticandidaistrains AT mariaaudilenedefreitas invitroantibioticandmodulatoryactivityofimesosphaerumsuaveolensilkuntzeagainsticandidaistrains AT vivianebezerradasilva invitroantibioticandmodulatoryactivityofimesosphaerumsuaveolensilkuntzeagainsticandidaistrains AT joaocruzneto invitroantibioticandmodulatoryactivityofimesosphaerumsuaveolensilkuntzeagainsticandidaistrains AT antoniathassyalucasdossantos invitroantibioticandmodulatoryactivityofimesosphaerumsuaveolensilkuntzeagainsticandidaistrains AT mariaflavianabezerramoraisbraga invitroantibioticandmodulatoryactivityofimesosphaerumsuaveolensilkuntzeagainsticandidaistrains AT leomaraandradedasilva invitroantibioticandmodulatoryactivityofimesosphaerumsuaveolensilkuntzeagainsticandidaistrains AT mariaivaneiderocha invitroantibioticandmodulatoryactivityofimesosphaerumsuaveolensilkuntzeagainsticandidaistrains AT jeanpaulkamdem invitroantibioticandmodulatoryactivityofimesosphaerumsuaveolensilkuntzeagainsticandidaistrains AT marcelloiriti invitroantibioticandmodulatoryactivityofimesosphaerumsuaveolensilkuntzeagainsticandidaistrains AT saravitalini invitroantibioticandmodulatoryactivityofimesosphaerumsuaveolensilkuntzeagainsticandidaistrains AT antoniaelieneduarte invitroantibioticandmodulatoryactivityofimesosphaerumsuaveolensilkuntzeagainsticandidaistrains AT luizmarivandobarros invitroantibioticandmodulatoryactivityofimesosphaerumsuaveolensilkuntzeagainsticandidaistrains |