Circadian programming of the ellipsoid body sleep homeostat in Drosophila
Homeostatic and circadian processes collaborate to appropriately time and consolidate sleep and wake. To understand how these processes are integrated, we scheduled brief sleep deprivation at different times of day in Drosophila and find elevated morning rebound compared to evening. These effects de...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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eLife Sciences Publications Ltd
2022-06-01
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| Series: | eLife |
| Subjects: | |
| Online Access: | https://elifesciences.org/articles/74327 |
| _version_ | 1828108370500911104 |
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| author | Tomas Andreani Clark Rosensweig Shiju Sisobhan Emmanuel Ogunlana William Kath Ravi Allada |
| author_facet | Tomas Andreani Clark Rosensweig Shiju Sisobhan Emmanuel Ogunlana William Kath Ravi Allada |
| author_sort | Tomas Andreani |
| collection | DOAJ |
| description | Homeostatic and circadian processes collaborate to appropriately time and consolidate sleep and wake. To understand how these processes are integrated, we scheduled brief sleep deprivation at different times of day in Drosophila and find elevated morning rebound compared to evening. These effects depend on discrete morning and evening clock neurons, independent of their roles in circadian locomotor activity. In the R5 ellipsoid body sleep homeostat, we identified elevated morning expression of activity dependent and presynaptic gene expression as well as the presynaptic protein BRUCHPILOT consistent with regulation by clock circuits. These neurons also display elevated calcium levels in response to sleep loss in the morning, but not the evening consistent with the observed time-dependent sleep rebound. These studies reveal the circuit and molecular mechanisms by which discrete circadian clock neurons program a homeostatic sleep center. |
| first_indexed | 2024-04-11T10:44:41Z |
| format | Article |
| id | doaj.art-a30b30deda2246d4bcc471aa54b92817 |
| institution | Directory Open Access Journal |
| issn | 2050-084X |
| language | English |
| last_indexed | 2024-04-11T10:44:41Z |
| publishDate | 2022-06-01 |
| publisher | eLife Sciences Publications Ltd |
| record_format | Article |
| series | eLife |
| spelling | doaj.art-a30b30deda2246d4bcc471aa54b928172022-12-22T04:29:05ZengeLife Sciences Publications LtdeLife2050-084X2022-06-011110.7554/eLife.74327Circadian programming of the ellipsoid body sleep homeostat in DrosophilaTomas Andreani0https://orcid.org/0000-0003-2967-9689Clark Rosensweig1https://orcid.org/0000-0001-6364-2025Shiju Sisobhan2https://orcid.org/0000-0002-9715-1029Emmanuel Ogunlana3https://orcid.org/0000-0002-7955-4115William Kath4Ravi Allada5https://orcid.org/0000-0003-4371-1577Department of Neurobiology, Northwestern University, Evanston, United StatesDepartment of Neurobiology, Northwestern University, Evanston, United StatesDepartment of Neurobiology, Northwestern University, Evanston, United StatesDepartment of Neurobiology, Northwestern University, Evanston, United StatesDepartment of Engineering Sciences and Applied Mathematics, Northwestern University, Evanston, United StatesDepartment of Neurobiology, Northwestern University, Chicago, United StatesHomeostatic and circadian processes collaborate to appropriately time and consolidate sleep and wake. To understand how these processes are integrated, we scheduled brief sleep deprivation at different times of day in Drosophila and find elevated morning rebound compared to evening. These effects depend on discrete morning and evening clock neurons, independent of their roles in circadian locomotor activity. In the R5 ellipsoid body sleep homeostat, we identified elevated morning expression of activity dependent and presynaptic gene expression as well as the presynaptic protein BRUCHPILOT consistent with regulation by clock circuits. These neurons also display elevated calcium levels in response to sleep loss in the morning, but not the evening consistent with the observed time-dependent sleep rebound. These studies reveal the circuit and molecular mechanisms by which discrete circadian clock neurons program a homeostatic sleep center.https://elifesciences.org/articles/74327circadiansleep homeostasisellipsoid bodyDrosophilaclock |
| spellingShingle | Tomas Andreani Clark Rosensweig Shiju Sisobhan Emmanuel Ogunlana William Kath Ravi Allada Circadian programming of the ellipsoid body sleep homeostat in Drosophila eLife circadian sleep homeostasis ellipsoid body Drosophila clock |
| title | Circadian programming of the ellipsoid body sleep homeostat in Drosophila |
| title_full | Circadian programming of the ellipsoid body sleep homeostat in Drosophila |
| title_fullStr | Circadian programming of the ellipsoid body sleep homeostat in Drosophila |
| title_full_unstemmed | Circadian programming of the ellipsoid body sleep homeostat in Drosophila |
| title_short | Circadian programming of the ellipsoid body sleep homeostat in Drosophila |
| title_sort | circadian programming of the ellipsoid body sleep homeostat in drosophila |
| topic | circadian sleep homeostasis ellipsoid body Drosophila clock |
| url | https://elifesciences.org/articles/74327 |
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