Preparation and Validation of Fe2O3 Modified Carbon Paste Electrode for Measurement of Dopamine by Voltammetry Method
Preparation and validation of the Fe2O3 modified carbon paste electrode have been carried out for the analysis of dopamine by the voltammetry method. Validation parameters of carbon paste electrodes (CPA) tested include linear concentration range, Limit of Detection (LoD), Limit of Quantification (L...
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| Format: | Article |
| Language: | English |
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Chemistry Department, Faculty of Sciences and Mathematics, Diponegoro University
2019-11-01
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| Series: | Jurnal Kimia Sains dan Aplikasi |
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| Online Access: | https://ejournal.undip.ac.id/index.php/ksa/article/view/24167 |
| _version_ | 1819284731172225024 |
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| author | Irdhawati Irdhawati Manuntun Manurung Ni Ketut Yuni Sri Lestari |
| author_facet | Irdhawati Irdhawati Manuntun Manurung Ni Ketut Yuni Sri Lestari |
| author_sort | Irdhawati Irdhawati |
| collection | DOAJ |
| description | Preparation and validation of the Fe2O3 modified carbon paste electrode have been carried out for the analysis of dopamine by the voltammetry method. Validation parameters of carbon paste electrodes (CPA) tested include linear concentration range, Limit of Detection (LoD), Limit of Quantification (LoQ), accuracy, and selectivity in the presence of ascorbic acid and uric acid. The results obtained in the optimization of the composition and condition of the Fe2O3 modifier in graphite are 0.8% mass at pH 6 solution with the Brinton Robinson buffer solution. The peak potential shifted 32 mV in the negative direction from the initial peak potential of 319 mV at the carbon paste electrode without modification to the peak potential of 287 mV at the 0.8% Fe2O3 modified carbon paste electrode. The linear concentration range at concentrations of 8 µM–100 µM obtained a linear equation y= 1.1204 + 0.1289x with R2=0.99943. LoD value obtained is 0.23 µM, and the LoQ is 0.77 µM. In the selectivity test obtained in the dopamine analysis using 0.8% Fe2O3 modified carbon paste electrode, the anodic peaks of dopamine (75 mV), ascorbic acid (190 mV), and uric acid (200 mV) can be separated. This method has acceptable repeatability because it produces a Horwitz Ratio value of 0.0417, which is smaller than two. Preparation and validation of 0.8% Fe2O3 modified carbon paste electrode can be declared as eligible for the measurement of dopamine. |
| first_indexed | 2024-12-24T01:52:02Z |
| format | Article |
| id | doaj.art-a30b82ff1f9946e9a2a76819e896c279 |
| institution | Directory Open Access Journal |
| issn | 1410-8917 2597-9914 |
| language | English |
| last_indexed | 2024-12-24T01:52:02Z |
| publishDate | 2019-11-01 |
| publisher | Chemistry Department, Faculty of Sciences and Mathematics, Diponegoro University |
| record_format | Article |
| series | Jurnal Kimia Sains dan Aplikasi |
| spelling | doaj.art-a30b82ff1f9946e9a2a76819e896c2792022-12-21T17:21:41ZengChemistry Department, Faculty of Sciences and Mathematics, Diponegoro UniversityJurnal Kimia Sains dan Aplikasi1410-89172597-99142019-11-0122622723410.14710/jksa.22.6.227-23415779Preparation and Validation of Fe2O3 Modified Carbon Paste Electrode for Measurement of Dopamine by Voltammetry MethodIrdhawati Irdhawati0https://orcid.org/0000-0002-8398-6287Manuntun Manurung1Ni Ketut Yuni Sri Lestari2Department of Chemistry, Faculty of Mathematics and Natural Sciences, Udayana University, IndonesiaDepartment of Chemistry, Faculty of Mathematics and Natural Sciences, Udayana University, IndonesiaDepartment of Chemistry, Faculty of Mathematics and Natural Sciences, Udayana University, IndonesiaPreparation and validation of the Fe2O3 modified carbon paste electrode have been carried out for the analysis of dopamine by the voltammetry method. Validation parameters of carbon paste electrodes (CPA) tested include linear concentration range, Limit of Detection (LoD), Limit of Quantification (LoQ), accuracy, and selectivity in the presence of ascorbic acid and uric acid. The results obtained in the optimization of the composition and condition of the Fe2O3 modifier in graphite are 0.8% mass at pH 6 solution with the Brinton Robinson buffer solution. The peak potential shifted 32 mV in the negative direction from the initial peak potential of 319 mV at the carbon paste electrode without modification to the peak potential of 287 mV at the 0.8% Fe2O3 modified carbon paste electrode. The linear concentration range at concentrations of 8 µM–100 µM obtained a linear equation y= 1.1204 + 0.1289x with R2=0.99943. LoD value obtained is 0.23 µM, and the LoQ is 0.77 µM. In the selectivity test obtained in the dopamine analysis using 0.8% Fe2O3 modified carbon paste electrode, the anodic peaks of dopamine (75 mV), ascorbic acid (190 mV), and uric acid (200 mV) can be separated. This method has acceptable repeatability because it produces a Horwitz Ratio value of 0.0417, which is smaller than two. Preparation and validation of 0.8% Fe2O3 modified carbon paste electrode can be declared as eligible for the measurement of dopamine.https://ejournal.undip.ac.id/index.php/ksa/article/view/24167carbon paste electrodedopaminefe2o3voltammetry |
| spellingShingle | Irdhawati Irdhawati Manuntun Manurung Ni Ketut Yuni Sri Lestari Preparation and Validation of Fe2O3 Modified Carbon Paste Electrode for Measurement of Dopamine by Voltammetry Method Jurnal Kimia Sains dan Aplikasi carbon paste electrode dopamine fe2o3 voltammetry |
| title | Preparation and Validation of Fe2O3 Modified Carbon Paste Electrode for Measurement of Dopamine by Voltammetry Method |
| title_full | Preparation and Validation of Fe2O3 Modified Carbon Paste Electrode for Measurement of Dopamine by Voltammetry Method |
| title_fullStr | Preparation and Validation of Fe2O3 Modified Carbon Paste Electrode for Measurement of Dopamine by Voltammetry Method |
| title_full_unstemmed | Preparation and Validation of Fe2O3 Modified Carbon Paste Electrode for Measurement of Dopamine by Voltammetry Method |
| title_short | Preparation and Validation of Fe2O3 Modified Carbon Paste Electrode for Measurement of Dopamine by Voltammetry Method |
| title_sort | preparation and validation of fe2o3 modified carbon paste electrode for measurement of dopamine by voltammetry method |
| topic | carbon paste electrode dopamine fe2o3 voltammetry |
| url | https://ejournal.undip.ac.id/index.php/ksa/article/view/24167 |
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