Effects of LL-37 on Gingival Fibroblasts: A Role in Periodontal Tissue Remodeling?
Mounting evidence suggests that the host defence peptide, LL-37, plays a role in both inflammation and in wound healing; however, the role of this peptide in the remodeling and maintenance of oral tissues is not yet fully understood. Fibroblasts are the most abundant cell type within the periodontal...
| Main Authors: | , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2018-07-01
|
| Series: | Vaccines |
| Subjects: | |
| Online Access: | http://www.mdpi.com/2076-393X/6/3/44 |
| _version_ | 1811308026515161088 |
|---|---|
| author | Maelíosa T. C. McCrudden Katherine O’Donnell Chris R. Irwin Fionnuala T. Lundy |
| author_facet | Maelíosa T. C. McCrudden Katherine O’Donnell Chris R. Irwin Fionnuala T. Lundy |
| author_sort | Maelíosa T. C. McCrudden |
| collection | DOAJ |
| description | Mounting evidence suggests that the host defence peptide, LL-37, plays a role in both inflammation and in wound healing; however, the role of this peptide in the remodeling and maintenance of oral tissues is not yet fully understood. Fibroblasts are the most abundant cell type within the periodontal tissues, and gingival fibroblasts play an important role in maintaining and repairing the gingival tissues which are constantly exposed to external insults. In this study we examined the direct effects of LL-37 treatment on gingival fibroblasts and found that LL-37 significantly increased secretion of both interleukin 8 (IL-8) and IL-6 from these cells. LL-37 tended to decrease matrix metalloproteinase (MMP) activity in gingival fibroblasts, but this decrease did not reach statistical significance. LL-37 significantly increased tissue inhibitor of metalloproteinase-1 (TIMP-1) production by gingival fibroblasts, but had no significant effect on TIMP-2 levels. LL-37 was also shown to significantly increase production of basic fibroblast growth factor (bFGF), hepatocyte growth factor (HGF), and keratinocyte growth factor (KGF) in gingival fibroblasts. Taken together, these results suggest an important role for the host defence peptide, LL-37, in modulating the fibroblast response to remodeling in periodontal tissues. |
| first_indexed | 2024-04-13T09:14:37Z |
| format | Article |
| id | doaj.art-a30daf95dbc146aea20f1c46c2eecb48 |
| institution | Directory Open Access Journal |
| issn | 2076-393X |
| language | English |
| last_indexed | 2024-04-13T09:14:37Z |
| publishDate | 2018-07-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Vaccines |
| spelling | doaj.art-a30daf95dbc146aea20f1c46c2eecb482022-12-22T02:52:46ZengMDPI AGVaccines2076-393X2018-07-01634410.3390/vaccines6030044vaccines6030044Effects of LL-37 on Gingival Fibroblasts: A Role in Periodontal Tissue Remodeling?Maelíosa T. C. McCrudden0Katherine O’Donnell1Chris R. Irwin2Fionnuala T. Lundy3Centre for Experimental Medicine, Wellcome-Wolfson Institute for Experimental Medicine, School of Medicine, Dentistry and Biomedical Sciences, Queen’s University Belfast, Belfast BT7 9BL, UKCentre for Dentistry, School of Medicine, Dentistry and Biomedical Sciences, Queen’s University Belfast, Belfast BT12 6BA, UKCentre for Dentistry, School of Medicine, Dentistry and Biomedical Sciences, Queen’s University Belfast, Belfast BT12 6BA, UKCentre for Experimental Medicine, Wellcome-Wolfson Institute for Experimental Medicine, School of Medicine, Dentistry and Biomedical Sciences, Queen’s University Belfast, Belfast BT7 9BL, UKMounting evidence suggests that the host defence peptide, LL-37, plays a role in both inflammation and in wound healing; however, the role of this peptide in the remodeling and maintenance of oral tissues is not yet fully understood. Fibroblasts are the most abundant cell type within the periodontal tissues, and gingival fibroblasts play an important role in maintaining and repairing the gingival tissues which are constantly exposed to external insults. In this study we examined the direct effects of LL-37 treatment on gingival fibroblasts and found that LL-37 significantly increased secretion of both interleukin 8 (IL-8) and IL-6 from these cells. LL-37 tended to decrease matrix metalloproteinase (MMP) activity in gingival fibroblasts, but this decrease did not reach statistical significance. LL-37 significantly increased tissue inhibitor of metalloproteinase-1 (TIMP-1) production by gingival fibroblasts, but had no significant effect on TIMP-2 levels. LL-37 was also shown to significantly increase production of basic fibroblast growth factor (bFGF), hepatocyte growth factor (HGF), and keratinocyte growth factor (KGF) in gingival fibroblasts. Taken together, these results suggest an important role for the host defence peptide, LL-37, in modulating the fibroblast response to remodeling in periodontal tissues.http://www.mdpi.com/2076-393X/6/3/44fibroblastgingivalgrowth factorshost defence peptideLL-37oralperiodontalwound repair |
| spellingShingle | Maelíosa T. C. McCrudden Katherine O’Donnell Chris R. Irwin Fionnuala T. Lundy Effects of LL-37 on Gingival Fibroblasts: A Role in Periodontal Tissue Remodeling? Vaccines fibroblast gingival growth factors host defence peptide LL-37 oral periodontal wound repair |
| title | Effects of LL-37 on Gingival Fibroblasts: A Role in Periodontal Tissue Remodeling? |
| title_full | Effects of LL-37 on Gingival Fibroblasts: A Role in Periodontal Tissue Remodeling? |
| title_fullStr | Effects of LL-37 on Gingival Fibroblasts: A Role in Periodontal Tissue Remodeling? |
| title_full_unstemmed | Effects of LL-37 on Gingival Fibroblasts: A Role in Periodontal Tissue Remodeling? |
| title_short | Effects of LL-37 on Gingival Fibroblasts: A Role in Periodontal Tissue Remodeling? |
| title_sort | effects of ll 37 on gingival fibroblasts a role in periodontal tissue remodeling |
| topic | fibroblast gingival growth factors host defence peptide LL-37 oral periodontal wound repair |
| url | http://www.mdpi.com/2076-393X/6/3/44 |
| work_keys_str_mv | AT maeliosatcmccrudden effectsofll37ongingivalfibroblastsaroleinperiodontaltissueremodeling AT katherineodonnell effectsofll37ongingivalfibroblastsaroleinperiodontaltissueremodeling AT chrisrirwin effectsofll37ongingivalfibroblastsaroleinperiodontaltissueremodeling AT fionnualatlundy effectsofll37ongingivalfibroblastsaroleinperiodontaltissueremodeling |