Enzymatic Electroanalytical Biosensor Based on <i>Maramiellus colocasiae</i> Fungus for Detection of Phytomarkers in Infusions and Green Tea Kombucha
In this work, we developed an enzymatic voltammetric biosensor for the determination of catechin and gallic acid in green tea and kombucha samples. The differential pulse voltammetry (DPV) methodology was optimized regarding the amount of crude enzyme extract, incubation time in the presence of the...
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MDPI AG
2021-03-01
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| Series: | Biosensors |
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| Online Access: | https://www.mdpi.com/2079-6374/11/3/91 |
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| author | Érica A. Batista Giovanna N. M. Silva Livia F. Sgobbi Fabio B. Machado Isaac Y. Macedo Emily K. Moreno Jerônimo R. Neto Paulo S. Scalize Eric S. Gil |
| author_facet | Érica A. Batista Giovanna N. M. Silva Livia F. Sgobbi Fabio B. Machado Isaac Y. Macedo Emily K. Moreno Jerônimo R. Neto Paulo S. Scalize Eric S. Gil |
| author_sort | Érica A. Batista |
| collection | DOAJ |
| description | In this work, we developed an enzymatic voltammetric biosensor for the determination of catechin and gallic acid in green tea and kombucha samples. The differential pulse voltammetry (DPV) methodology was optimized regarding the amount of crude enzyme extract, incubation time in the presence of the substrates, optimal pH, reuse of the biosensor, and storage time. Samples of green tea and kombucha were purchased in local markets in the city of Goiânia-GO, Brazil. High performance liquid chromatography (HPLC) and Folin-Ciocalteu spectrophotometric techniques were performed for the comparison of the analytical methods employed. In addition, two calibration curves were made, one for catechin with a linear range from 1 to 60 µM (I = −0.152 * (catechin) − 1.846), with a detection limit of 0.12 µM and a quantification limit of 0.38 µM and one for gallic acid with a linear range from 3 to 60 µM (I = −0.0415 * (gallic acid) − 0.0572), with a detection limit of 0.14 µM and a quantification limit of 0.42 µM. The proposed biosensor was efficient in the determination of phenolic compounds in green tea. |
| first_indexed | 2024-03-10T13:01:10Z |
| format | Article |
| id | doaj.art-a30f5b0783f14e04a1fd1db58a715316 |
| institution | Directory Open Access Journal |
| issn | 2079-6374 |
| language | English |
| last_indexed | 2024-03-10T13:01:10Z |
| publishDate | 2021-03-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Biosensors |
| spelling | doaj.art-a30f5b0783f14e04a1fd1db58a7153162023-11-21T11:33:15ZengMDPI AGBiosensors2079-63742021-03-011139110.3390/bios11030091Enzymatic Electroanalytical Biosensor Based on <i>Maramiellus colocasiae</i> Fungus for Detection of Phytomarkers in Infusions and Green Tea KombuchaÉrica A. Batista0Giovanna N. M. Silva1Livia F. Sgobbi2Fabio B. Machado3Isaac Y. Macedo4Emily K. Moreno5Jerônimo R. Neto6Paulo S. Scalize7Eric S. Gil8Faculdade de Farmácia (FF), Universidade Federal de Goiás (UFG), Goiânia 74605-170, BrazilFaculdade de Farmácia (FF), Universidade Federal de Goiás (UFG), Goiânia 74605-170, BrazilInstituto de Química (IQ), Universidade Federal de Goiás (UFG), Goiânia 74690-900, BrazilFaculdade de Farmácia (FF), Universidade Federal de Goiás (UFG), Goiânia 74605-170, BrazilFaculdade de Farmácia (FF), Universidade Federal de Goiás (UFG), Goiânia 74605-170, BrazilFaculdade de Farmácia (FF), Universidade Federal de Goiás (UFG), Goiânia 74605-170, BrazilFaculdade de Farmácia (FF), Universidade Federal de Goiás (UFG), Goiânia 74605-170, BrazilEscola de Engenharia Civil e Ambiental (EECA), Universidade Federal de Goiás (UFG), Goiânia 74605-220, BrazilFaculdade de Farmácia (FF), Universidade Federal de Goiás (UFG), Goiânia 74605-170, BrazilIn this work, we developed an enzymatic voltammetric biosensor for the determination of catechin and gallic acid in green tea and kombucha samples. The differential pulse voltammetry (DPV) methodology was optimized regarding the amount of crude enzyme extract, incubation time in the presence of the substrates, optimal pH, reuse of the biosensor, and storage time. Samples of green tea and kombucha were purchased in local markets in the city of Goiânia-GO, Brazil. High performance liquid chromatography (HPLC) and Folin-Ciocalteu spectrophotometric techniques were performed for the comparison of the analytical methods employed. In addition, two calibration curves were made, one for catechin with a linear range from 1 to 60 µM (I = −0.152 * (catechin) − 1.846), with a detection limit of 0.12 µM and a quantification limit of 0.38 µM and one for gallic acid with a linear range from 3 to 60 µM (I = −0.0415 * (gallic acid) − 0.0572), with a detection limit of 0.14 µM and a quantification limit of 0.42 µM. The proposed biosensor was efficient in the determination of phenolic compounds in green tea.https://www.mdpi.com/2079-6374/11/3/91quality controlpolyphenoloxidase enzymeskombuchagreen teaelectrochemical biosensor |
| spellingShingle | Érica A. Batista Giovanna N. M. Silva Livia F. Sgobbi Fabio B. Machado Isaac Y. Macedo Emily K. Moreno Jerônimo R. Neto Paulo S. Scalize Eric S. Gil Enzymatic Electroanalytical Biosensor Based on <i>Maramiellus colocasiae</i> Fungus for Detection of Phytomarkers in Infusions and Green Tea Kombucha Biosensors quality control polyphenoloxidase enzymes kombucha green tea electrochemical biosensor |
| title | Enzymatic Electroanalytical Biosensor Based on <i>Maramiellus colocasiae</i> Fungus for Detection of Phytomarkers in Infusions and Green Tea Kombucha |
| title_full | Enzymatic Electroanalytical Biosensor Based on <i>Maramiellus colocasiae</i> Fungus for Detection of Phytomarkers in Infusions and Green Tea Kombucha |
| title_fullStr | Enzymatic Electroanalytical Biosensor Based on <i>Maramiellus colocasiae</i> Fungus for Detection of Phytomarkers in Infusions and Green Tea Kombucha |
| title_full_unstemmed | Enzymatic Electroanalytical Biosensor Based on <i>Maramiellus colocasiae</i> Fungus for Detection of Phytomarkers in Infusions and Green Tea Kombucha |
| title_short | Enzymatic Electroanalytical Biosensor Based on <i>Maramiellus colocasiae</i> Fungus for Detection of Phytomarkers in Infusions and Green Tea Kombucha |
| title_sort | enzymatic electroanalytical biosensor based on i maramiellus colocasiae i fungus for detection of phytomarkers in infusions and green tea kombucha |
| topic | quality control polyphenoloxidase enzymes kombucha green tea electrochemical biosensor |
| url | https://www.mdpi.com/2079-6374/11/3/91 |
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