Application progress of single-cell sequencing technology in mesenchymal stem cells research
Single-Cell Sequencing (SCS) technology plays an important role in the field of Mesenchymal Stem Cells (MSCs) research. This paper comprehensively describes the application of SCS technology in the field of MSCs research, including (1) SCS enables more precise MSCs characterization and biomarker def...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2024-01-01
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| Series: | Frontiers in Cell and Developmental Biology |
| Subjects: | |
| Online Access: | https://www.frontiersin.org/articles/10.3389/fcell.2023.1336482/full |
| _version_ | 1827386871422910464 |
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| author | Hao Li Yusong Wang Gehua Zhu Qimin Ma Shengyu Huang Guanghua Guo Feng Zhu Feng Zhu |
| author_facet | Hao Li Yusong Wang Gehua Zhu Qimin Ma Shengyu Huang Guanghua Guo Feng Zhu Feng Zhu |
| author_sort | Hao Li |
| collection | DOAJ |
| description | Single-Cell Sequencing (SCS) technology plays an important role in the field of Mesenchymal Stem Cells (MSCs) research. This paper comprehensively describes the application of SCS technology in the field of MSCs research, including (1) SCS enables more precise MSCs characterization and biomarker definition. (2) SCS reveals the prevalent gene expression heterogeneity among different subclusters within MSCs, which contributes to a more comprehensive understanding of MSCs function and diversity in developmental, regenerative, and pathological contexts. (3) SCS provides insights into the dynamic transcriptional changes experienced by MSCs during differentiation and the complex web of important signaling pathways and regulatory factors controlling key processes within MSCs, including proliferation, differentiation and regulation, and interactions mechanisms. (4) The analytical methods underpinning SCS data are rapidly evolving and converging with the field of histological research to systematically deconstruct the functions and mechanisms of MSCs. This review provides new perspectives for unraveling the biological properties, heterogeneity, differentiation potential, biological functions, and clinical potential of MSCs at the single-cell level. |
| first_indexed | 2024-03-08T15:52:54Z |
| format | Article |
| id | doaj.art-a30fd83dea884e7da25b86f9c942013c |
| institution | Directory Open Access Journal |
| issn | 2296-634X |
| language | English |
| last_indexed | 2024-03-08T15:52:54Z |
| publishDate | 2024-01-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Cell and Developmental Biology |
| spelling | doaj.art-a30fd83dea884e7da25b86f9c942013c2024-01-09T04:11:20ZengFrontiers Media S.A.Frontiers in Cell and Developmental Biology2296-634X2024-01-011110.3389/fcell.2023.13364821336482Application progress of single-cell sequencing technology in mesenchymal stem cells researchHao Li0Yusong Wang1Gehua Zhu2Qimin Ma3Shengyu Huang4Guanghua Guo5Feng Zhu6Feng Zhu7Medical Center of Burn Plastic and Wound Repair, The First Affiliated Hospital, Jiangxi Medical College, Nanchang University, Nanchang, ChinaDepartment of Burns, The First Affiliated Hospital, Naval Medical University, Shanghai, ChinaMedical Center of Burn Plastic and Wound Repair, The First Affiliated Hospital, Jiangxi Medical College, Nanchang University, Nanchang, ChinaDepartment of Critical Care Medicine, Shanghai East Hospital, Tongji University School of Medicine, Shanghai, ChinaMedical Center of Burn Plastic and Wound Repair, The First Affiliated Hospital, Jiangxi Medical College, Nanchang University, Nanchang, ChinaMedical Center of Burn Plastic and Wound Repair, The First Affiliated Hospital, Jiangxi Medical College, Nanchang University, Nanchang, ChinaDepartment of Burns, The First Affiliated Hospital, Naval Medical University, Shanghai, ChinaDepartment of Critical Care Medicine, Shanghai East Hospital, Tongji University School of Medicine, Shanghai, ChinaSingle-Cell Sequencing (SCS) technology plays an important role in the field of Mesenchymal Stem Cells (MSCs) research. This paper comprehensively describes the application of SCS technology in the field of MSCs research, including (1) SCS enables more precise MSCs characterization and biomarker definition. (2) SCS reveals the prevalent gene expression heterogeneity among different subclusters within MSCs, which contributes to a more comprehensive understanding of MSCs function and diversity in developmental, regenerative, and pathological contexts. (3) SCS provides insights into the dynamic transcriptional changes experienced by MSCs during differentiation and the complex web of important signaling pathways and regulatory factors controlling key processes within MSCs, including proliferation, differentiation and regulation, and interactions mechanisms. (4) The analytical methods underpinning SCS data are rapidly evolving and converging with the field of histological research to systematically deconstruct the functions and mechanisms of MSCs. This review provides new perspectives for unraveling the biological properties, heterogeneity, differentiation potential, biological functions, and clinical potential of MSCs at the single-cell level.https://www.frontiersin.org/articles/10.3389/fcell.2023.1336482/fullsingle-cell sequencingmesenchymal stem cellssurface markersdifferentiation trajectoriesimmunomodulation |
| spellingShingle | Hao Li Yusong Wang Gehua Zhu Qimin Ma Shengyu Huang Guanghua Guo Feng Zhu Feng Zhu Application progress of single-cell sequencing technology in mesenchymal stem cells research Frontiers in Cell and Developmental Biology single-cell sequencing mesenchymal stem cells surface markers differentiation trajectories immunomodulation |
| title | Application progress of single-cell sequencing technology in mesenchymal stem cells research |
| title_full | Application progress of single-cell sequencing technology in mesenchymal stem cells research |
| title_fullStr | Application progress of single-cell sequencing technology in mesenchymal stem cells research |
| title_full_unstemmed | Application progress of single-cell sequencing technology in mesenchymal stem cells research |
| title_short | Application progress of single-cell sequencing technology in mesenchymal stem cells research |
| title_sort | application progress of single cell sequencing technology in mesenchymal stem cells research |
| topic | single-cell sequencing mesenchymal stem cells surface markers differentiation trajectories immunomodulation |
| url | https://www.frontiersin.org/articles/10.3389/fcell.2023.1336482/full |
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