In Search of Antioxidant Peptides from Porcine Liver Hydrolysates Using Analytical and Peptidomic Approach
The search for antioxidant peptides as health-promoting agents is of great scientific interest for their biotechnological applications. Thus, the main goal of this study was to identify antioxidant peptides from pork liver using alcalase, bromelain, flavourzyme, and papain enzymes. All liver hydroly...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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MDPI AG
2021-12-01
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| Series: | Antioxidants |
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| Online Access: | https://www.mdpi.com/2076-3921/11/1/27 |
| _version_ | 1797496252799123456 |
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| author | María López-Pedrouso José M. Lorenzo Paula Borrajo Daniel Franco |
| author_facet | María López-Pedrouso José M. Lorenzo Paula Borrajo Daniel Franco |
| author_sort | María López-Pedrouso |
| collection | DOAJ |
| description | The search for antioxidant peptides as health-promoting agents is of great scientific interest for their biotechnological applications. Thus, the main goal of this study was to identify antioxidant peptides from pork liver using alcalase, bromelain, flavourzyme, and papain enzymes. All liver hydrolysates proved to be of adequate quality regarding the ratio EAA/NEAA, particularly flavourzyme hydrolysates. The peptidomic profiles were significantly different for each enzyme and their characterizations were performed, resulting in forty-four differentially abundant peptides among the four treatments. Porcine liver hydrolysates from alcalase and bromelain are demonstrated to have the most antioxidant capacity. On the other hand, hydrophobic amino acid residues (serine, threonine, histidine and aspartic acid) might be reducing the hydrolysates antioxidant capacity. Seventeen peptides from collagen, albumin, globin domain-containing protein, cytochrome β, fructose-bisphosphate aldolase, dihydropyrimidinase, argininosuccinate synthase, and ATP synthase seem to be antioxidant. Further studies are necessary to isolate these peptides and test them in in vivo experiments. |
| first_indexed | 2024-03-10T03:01:59Z |
| format | Article |
| id | doaj.art-a311d64e78e544eba2c1c8e7681418a4 |
| institution | Directory Open Access Journal |
| issn | 2076-3921 |
| language | English |
| last_indexed | 2024-03-10T03:01:59Z |
| publishDate | 2021-12-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Antioxidants |
| spelling | doaj.art-a311d64e78e544eba2c1c8e7681418a42023-11-23T12:46:10ZengMDPI AGAntioxidants2076-39212021-12-011112710.3390/antiox11010027In Search of Antioxidant Peptides from Porcine Liver Hydrolysates Using Analytical and Peptidomic ApproachMaría López-Pedrouso0José M. Lorenzo1Paula Borrajo2Daniel Franco3Departamento de Zooloxía, Xenética e Antropoloxía Física, Universidade de Santiago de Compostela, 15872 Santiago de Compostela, SpainCentro Tecnolóxico da Carne de Galicia, Rúa Galicia No. 4, Parque Tecnolóxico de Galicia, San Cibrao das Viñas, 32900 Ourense, SpainCentro Tecnolóxico da Carne de Galicia, Rúa Galicia No. 4, Parque Tecnolóxico de Galicia, San Cibrao das Viñas, 32900 Ourense, SpainCentro Tecnolóxico da Carne de Galicia, Rúa Galicia No. 4, Parque Tecnolóxico de Galicia, San Cibrao das Viñas, 32900 Ourense, SpainThe search for antioxidant peptides as health-promoting agents is of great scientific interest for their biotechnological applications. Thus, the main goal of this study was to identify antioxidant peptides from pork liver using alcalase, bromelain, flavourzyme, and papain enzymes. All liver hydrolysates proved to be of adequate quality regarding the ratio EAA/NEAA, particularly flavourzyme hydrolysates. The peptidomic profiles were significantly different for each enzyme and their characterizations were performed, resulting in forty-four differentially abundant peptides among the four treatments. Porcine liver hydrolysates from alcalase and bromelain are demonstrated to have the most antioxidant capacity. On the other hand, hydrophobic amino acid residues (serine, threonine, histidine and aspartic acid) might be reducing the hydrolysates antioxidant capacity. Seventeen peptides from collagen, albumin, globin domain-containing protein, cytochrome β, fructose-bisphosphate aldolase, dihydropyrimidinase, argininosuccinate synthase, and ATP synthase seem to be antioxidant. Further studies are necessary to isolate these peptides and test them in in vivo experiments.https://www.mdpi.com/2076-3921/11/1/27amino acid profilealcalasebromelainflavourzymepapainpork by-products |
| spellingShingle | María López-Pedrouso José M. Lorenzo Paula Borrajo Daniel Franco In Search of Antioxidant Peptides from Porcine Liver Hydrolysates Using Analytical and Peptidomic Approach Antioxidants amino acid profile alcalase bromelain flavourzyme papain pork by-products |
| title | In Search of Antioxidant Peptides from Porcine Liver Hydrolysates Using Analytical and Peptidomic Approach |
| title_full | In Search of Antioxidant Peptides from Porcine Liver Hydrolysates Using Analytical and Peptidomic Approach |
| title_fullStr | In Search of Antioxidant Peptides from Porcine Liver Hydrolysates Using Analytical and Peptidomic Approach |
| title_full_unstemmed | In Search of Antioxidant Peptides from Porcine Liver Hydrolysates Using Analytical and Peptidomic Approach |
| title_short | In Search of Antioxidant Peptides from Porcine Liver Hydrolysates Using Analytical and Peptidomic Approach |
| title_sort | in search of antioxidant peptides from porcine liver hydrolysates using analytical and peptidomic approach |
| topic | amino acid profile alcalase bromelain flavourzyme papain pork by-products |
| url | https://www.mdpi.com/2076-3921/11/1/27 |
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