Recovery of a hypolipidemic polysaccharide from artificially cultivated Sanghuangporus vaninii with an effective method
In this study, an effective method was developed to extract the polysaccharide from Sanghuangporus vaninii (PFSV) by destroying the cell wall. Box-Behnken design was employed to determine the optimal processing conditions as follows: processing temperature (80°C), processing time (0.81 h) and amount...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2023-01-01
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| Series: | Frontiers in Nutrition |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fnut.2022.1095556/full |
| _version_ | 1797954039061676032 |
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| author | Zuo-fa Zhang Ting-ting Song Jian-fei Chen Guo-ying Lv |
| author_facet | Zuo-fa Zhang Ting-ting Song Jian-fei Chen Guo-ying Lv |
| author_sort | Zuo-fa Zhang |
| collection | DOAJ |
| description | In this study, an effective method was developed to extract the polysaccharide from Sanghuangporus vaninii (PFSV) by destroying the cell wall. Box-Behnken design was employed to determine the optimal processing conditions as follows: processing temperature (80°C), processing time (0.81 h) and amount of HCl (1.5 ml). Under these conditions, the yield of PFSV reached 5.94 ± 0.16%. The purified polysaccharide (PFSV-2) was found to be a hetero-polysaccharide with an average molecular weight of 20.377 kDa. The backbone of PFSV-2 was composed of an →6)-α-Galp-(1→ and →2,6)-β-Manp-(1→ and →2)-α-Fucp-(1→ and was branched of t-α-Manp-(1→ at position 2 of residue B. PFSV-2 showed hypolipidemic activity by decreasing lipid accumulation and the levels of total cholesterol and triglycerides in zebrafish larvae. Furthermore, PFSV-2 downregulated the pparg, fasn, and HMGCRb genes and upregulated the pparab and acaca genes. These findings suggested PFSV-2 may be a promising candidate in lipid regulation therapy. |
| first_indexed | 2024-04-10T23:11:21Z |
| format | Article |
| id | doaj.art-a3134ae3e57b40ccb4b7d40a6d38eac2 |
| institution | Directory Open Access Journal |
| issn | 2296-861X |
| language | English |
| last_indexed | 2024-04-10T23:11:21Z |
| publishDate | 2023-01-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Nutrition |
| spelling | doaj.art-a3134ae3e57b40ccb4b7d40a6d38eac22023-01-13T05:26:02ZengFrontiers Media S.A.Frontiers in Nutrition2296-861X2023-01-01910.3389/fnut.2022.10955561095556Recovery of a hypolipidemic polysaccharide from artificially cultivated Sanghuangporus vaninii with an effective methodZuo-fa ZhangTing-ting SongJian-fei ChenGuo-ying LvIn this study, an effective method was developed to extract the polysaccharide from Sanghuangporus vaninii (PFSV) by destroying the cell wall. Box-Behnken design was employed to determine the optimal processing conditions as follows: processing temperature (80°C), processing time (0.81 h) and amount of HCl (1.5 ml). Under these conditions, the yield of PFSV reached 5.94 ± 0.16%. The purified polysaccharide (PFSV-2) was found to be a hetero-polysaccharide with an average molecular weight of 20.377 kDa. The backbone of PFSV-2 was composed of an →6)-α-Galp-(1→ and →2,6)-β-Manp-(1→ and →2)-α-Fucp-(1→ and was branched of t-α-Manp-(1→ at position 2 of residue B. PFSV-2 showed hypolipidemic activity by decreasing lipid accumulation and the levels of total cholesterol and triglycerides in zebrafish larvae. Furthermore, PFSV-2 downregulated the pparg, fasn, and HMGCRb genes and upregulated the pparab and acaca genes. These findings suggested PFSV-2 may be a promising candidate in lipid regulation therapy.https://www.frontiersin.org/articles/10.3389/fnut.2022.1095556/fullSanghuangporus vaniniihypolipidemic polysaccharideacid-ethanol pretreatmentzebrafish modelstructure illustration |
| spellingShingle | Zuo-fa Zhang Ting-ting Song Jian-fei Chen Guo-ying Lv Recovery of a hypolipidemic polysaccharide from artificially cultivated Sanghuangporus vaninii with an effective method Frontiers in Nutrition Sanghuangporus vaninii hypolipidemic polysaccharide acid-ethanol pretreatment zebrafish model structure illustration |
| title | Recovery of a hypolipidemic polysaccharide from artificially cultivated Sanghuangporus vaninii with an effective method |
| title_full | Recovery of a hypolipidemic polysaccharide from artificially cultivated Sanghuangporus vaninii with an effective method |
| title_fullStr | Recovery of a hypolipidemic polysaccharide from artificially cultivated Sanghuangporus vaninii with an effective method |
| title_full_unstemmed | Recovery of a hypolipidemic polysaccharide from artificially cultivated Sanghuangporus vaninii with an effective method |
| title_short | Recovery of a hypolipidemic polysaccharide from artificially cultivated Sanghuangporus vaninii with an effective method |
| title_sort | recovery of a hypolipidemic polysaccharide from artificially cultivated sanghuangporus vaninii with an effective method |
| topic | Sanghuangporus vaninii hypolipidemic polysaccharide acid-ethanol pretreatment zebrafish model structure illustration |
| url | https://www.frontiersin.org/articles/10.3389/fnut.2022.1095556/full |
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