Nontargeted and targeted metabolomics analysis provides novel insight into nonvolatile metabolites in Jianghua Kucha tea germplasm (Camellia sinensis var. Assamica cv. Jianghua)
Jianghua Kucha (JHKC) is a special tea germplasm with high bitterness growing in China; however, the chemical characteristics of JHKC are not completely understood. In this study, 61 differential metabolites were identified between 11 wild JHKC individuals and 3 control cultivars of Fudingdabai, Yun...
| Main Authors: | , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2022-03-01
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| Series: | Food Chemistry: X |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2590157522000682 |
| _version_ | 1828289307952021504 |
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| author | Wenliang Wu Meiling Lu Jiakun Peng Haipeng Lv Jiang Shi Shuguang Zhang Zhen Liu Jihua Duan Dan Chen Weidong Dai Zhi Lin |
| author_facet | Wenliang Wu Meiling Lu Jiakun Peng Haipeng Lv Jiang Shi Shuguang Zhang Zhen Liu Jihua Duan Dan Chen Weidong Dai Zhi Lin |
| author_sort | Wenliang Wu |
| collection | DOAJ |
| description | Jianghua Kucha (JHKC) is a special tea germplasm with high bitterness growing in China; however, the chemical characteristics of JHKC are not completely understood. In this study, 61 differential metabolites were identified between 11 wild JHKC individuals and 3 control cultivars of Fudingdabai, Yunkang 10, and Zhuyeqi using comprehensive nontargeted and targeted metabolomics approach. The JHKC accessions mainly possessed significantly higher levels of purine alkaloids of theacrine (12.06 ± 5.23 mg/g) and 1,3,7-trimethyluric acid, non-epi-form flavanols (catechin, gallocatechin, catechin gallate, and gallocatechin gallate), and methylated flavanols of epigallocatechin-3-O-(3″-O-methyl)-gallate (4.79 ± 1.45 mg/g) and epicatechin-3-O-(3″-O-methyl)-gallate (1.02 ± 0.34 mg/g), as well as significantly lower levels of flavonol glycosides, which indicated that caffeine metabolism, flavonoid biosynthesis, and flavonol and flavone biosynthesis are mostly differential metabolic pathways. Our study demonstrated that JHKC germplasm is a promising resource for breeding novel tea cultivars with high contents of theacrine, non-epi-form flavanols, and methylated flavanols. |
| first_indexed | 2024-04-13T10:18:43Z |
| format | Article |
| id | doaj.art-a3153e4752c84c74b8649317af3c4b0c |
| institution | Directory Open Access Journal |
| issn | 2590-1575 |
| language | English |
| last_indexed | 2024-04-13T10:18:43Z |
| publishDate | 2022-03-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Food Chemistry: X |
| spelling | doaj.art-a3153e4752c84c74b8649317af3c4b0c2022-12-22T02:50:35ZengElsevierFood Chemistry: X2590-15752022-03-0113100270Nontargeted and targeted metabolomics analysis provides novel insight into nonvolatile metabolites in Jianghua Kucha tea germplasm (Camellia sinensis var. Assamica cv. Jianghua)Wenliang Wu0Meiling Lu1Jiakun Peng2Haipeng Lv3Jiang Shi4Shuguang Zhang5Zhen Liu6Jihua Duan7Dan Chen8Weidong Dai9Zhi Lin10Key Laboratory of Tea Biology and Resources Utilization, Ministry of Agriculture, Tea Research Institute, Chinese Academy of Agricultural Sciences, 9 Meiling South Road, Hangzhou, Zhejiang 310008, PR China; Tea Research Institute, Hunan Academy of Agricultural Sciences, Changsha, Hunan 410125, PR ChinaAgilent Technologies (China) Limited, 3 Wangjing North Road, Chaoyang District, Beijing 100102, PR ChinaKey Laboratory of Tea Biology and Resources Utilization, Ministry of Agriculture, Tea Research Institute, Chinese Academy of Agricultural Sciences, 9 Meiling South Road, Hangzhou, Zhejiang 310008, PR ChinaKey Laboratory of Tea Biology and Resources Utilization, Ministry of Agriculture, Tea Research Institute, Chinese Academy of Agricultural Sciences, 9 Meiling South Road, Hangzhou, Zhejiang 310008, PR ChinaKey Laboratory of Tea Biology and Resources Utilization, Ministry of Agriculture, Tea Research Institute, Chinese Academy of Agricultural Sciences, 9 Meiling South Road, Hangzhou, Zhejiang 310008, PR ChinaTea Research Institute, Hunan Academy of Agricultural Sciences, Changsha, Hunan 410125, PR ChinaTea Research Institute, Hunan Academy of Agricultural Sciences, Changsha, Hunan 410125, PR ChinaTea Research Institute, Hunan Academy of Agricultural Sciences, Changsha, Hunan 410125, PR ChinaKey Laboratory of Tea Biology and Resources Utilization, Ministry of Agriculture, Tea Research Institute, Chinese Academy of Agricultural Sciences, 9 Meiling South Road, Hangzhou, Zhejiang 310008, PR ChinaKey Laboratory of Tea Biology and Resources Utilization, Ministry of Agriculture, Tea Research Institute, Chinese Academy of Agricultural Sciences, 9 Meiling South Road, Hangzhou, Zhejiang 310008, PR China; Corresponding authors.Key Laboratory of Tea Biology and Resources Utilization, Ministry of Agriculture, Tea Research Institute, Chinese Academy of Agricultural Sciences, 9 Meiling South Road, Hangzhou, Zhejiang 310008, PR China; Corresponding authors.Jianghua Kucha (JHKC) is a special tea germplasm with high bitterness growing in China; however, the chemical characteristics of JHKC are not completely understood. In this study, 61 differential metabolites were identified between 11 wild JHKC individuals and 3 control cultivars of Fudingdabai, Yunkang 10, and Zhuyeqi using comprehensive nontargeted and targeted metabolomics approach. The JHKC accessions mainly possessed significantly higher levels of purine alkaloids of theacrine (12.06 ± 5.23 mg/g) and 1,3,7-trimethyluric acid, non-epi-form flavanols (catechin, gallocatechin, catechin gallate, and gallocatechin gallate), and methylated flavanols of epigallocatechin-3-O-(3″-O-methyl)-gallate (4.79 ± 1.45 mg/g) and epicatechin-3-O-(3″-O-methyl)-gallate (1.02 ± 0.34 mg/g), as well as significantly lower levels of flavonol glycosides, which indicated that caffeine metabolism, flavonoid biosynthesis, and flavonol and flavone biosynthesis are mostly differential metabolic pathways. Our study demonstrated that JHKC germplasm is a promising resource for breeding novel tea cultivars with high contents of theacrine, non-epi-form flavanols, and methylated flavanols.http://www.sciencedirect.com/science/article/pii/S2590157522000682Jianghua KuchaMetabolomicsNonvolatile metabolitesTheacrineLC-MS |
| spellingShingle | Wenliang Wu Meiling Lu Jiakun Peng Haipeng Lv Jiang Shi Shuguang Zhang Zhen Liu Jihua Duan Dan Chen Weidong Dai Zhi Lin Nontargeted and targeted metabolomics analysis provides novel insight into nonvolatile metabolites in Jianghua Kucha tea germplasm (Camellia sinensis var. Assamica cv. Jianghua) Food Chemistry: X Jianghua Kucha Metabolomics Nonvolatile metabolites Theacrine LC-MS |
| title | Nontargeted and targeted metabolomics analysis provides novel insight into nonvolatile metabolites in Jianghua Kucha tea germplasm (Camellia sinensis var. Assamica cv. Jianghua) |
| title_full | Nontargeted and targeted metabolomics analysis provides novel insight into nonvolatile metabolites in Jianghua Kucha tea germplasm (Camellia sinensis var. Assamica cv. Jianghua) |
| title_fullStr | Nontargeted and targeted metabolomics analysis provides novel insight into nonvolatile metabolites in Jianghua Kucha tea germplasm (Camellia sinensis var. Assamica cv. Jianghua) |
| title_full_unstemmed | Nontargeted and targeted metabolomics analysis provides novel insight into nonvolatile metabolites in Jianghua Kucha tea germplasm (Camellia sinensis var. Assamica cv. Jianghua) |
| title_short | Nontargeted and targeted metabolomics analysis provides novel insight into nonvolatile metabolites in Jianghua Kucha tea germplasm (Camellia sinensis var. Assamica cv. Jianghua) |
| title_sort | nontargeted and targeted metabolomics analysis provides novel insight into nonvolatile metabolites in jianghua kucha tea germplasm camellia sinensis var assamica cv jianghua |
| topic | Jianghua Kucha Metabolomics Nonvolatile metabolites Theacrine LC-MS |
| url | http://www.sciencedirect.com/science/article/pii/S2590157522000682 |
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