Dracocephalum moldavica L. Extract ameliorates intestinal inflammation by regulating gut microbiota and repairing the intestinal barrier in 2k1c rats
When used in ethnomedicine, Dracocephalum moldavica L. (DML) and its bioactive extract protect against several diseases caused by inflammation and oxidative stress. However, the effect of DML on intestinal inflammation in hypertension has not been thoroughly investigated. This study focused on the e...
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| Format: | Article |
| Language: | English |
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University of Belgrade, University of Novi Sad
2023-01-01
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| Series: | Archives of Biological Sciences |
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| Online Access: | https://doiserbia.nb.rs/img/doi/0354-4664/2023/0354-46642300013Y.pdf |
| _version_ | 1797393749393801216 |
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| author | Yu Hui Chen Zhangjun Chen Huixia Wang Zhanli |
| author_facet | Yu Hui Chen Zhangjun Chen Huixia Wang Zhanli |
| author_sort | Yu Hui |
| collection | DOAJ |
| description | When used in ethnomedicine, Dracocephalum moldavica L. (DML) and its bioactive extract protect against several diseases caused by inflammation and oxidative stress. However, the effect of DML on intestinal inflammation in hypertension has not been thoroughly investigated. This study focused on the effects of DML extract on intestinal inflammation in two-kidney, one clip (2K1C) rats. Male Wistar rats were divided into three groups with daily intragastric administration of either saline (control group and model group) or DML extract (treatment group, 50 mg/kg per day) for 4 weeks. In the model and treatment groups, 2K1C hypertension was induced by clipping the left renal artery. The profiles of stool microbiota, the levels of short-chain fatty acids (SCFAs), the integrity of the gut barrier, oxidative stress biomarkers and inflammatory factors were analyzed in rats supplemented with or without DML extract. This study revealed the alleviation of high blood pressure in 2K1C rats treated with DML extract. The DML extract attenuated gut microbiota dysbiosis in the 2K1C rats by increasing the abundance of the genus Lactobacillus and by decreasing the abundance of Papillibacter, Desulfovibrio and Anaerobiospirillum genera. Treatment with the DML extract increased the levels of SCFAs and repaired the intestinal barrier, it reduced the release of oxidative stress biomarkers and inflammatory factors and suppressed the expression of TLR4 and NF-κB mRNAs. We conclude that the DML extract may alleviate intestinal inflammation by adjusting the profile of gut microbiota and enhancing the intestinal barrier. |
| first_indexed | 2024-03-09T00:07:23Z |
| format | Article |
| id | doaj.art-a31781231c4040e18bb5fec265d36c16 |
| institution | Directory Open Access Journal |
| issn | 0354-4664 1821-4339 |
| language | English |
| last_indexed | 2024-03-09T00:07:23Z |
| publishDate | 2023-01-01 |
| publisher | University of Belgrade, University of Novi Sad |
| record_format | Article |
| series | Archives of Biological Sciences |
| spelling | doaj.art-a31781231c4040e18bb5fec265d36c162023-12-12T13:06:41ZengUniversity of Belgrade, University of Novi SadArchives of Biological Sciences0354-46641821-43392023-01-0175215516410.2298/ABS230307013Y0354-46642300013YDracocephalum moldavica L. Extract ameliorates intestinal inflammation by regulating gut microbiota and repairing the intestinal barrier in 2k1c ratsYu Hui0Chen Zhangjun1Chen Huixia2Wang Zhanli3School of Basic Medicine, Baotou Medical College, Baotou, ChinaSchool of Basic Medicine, Baotou Medical College, Baotou, ChinaSchool of Basic Medicine, Baotou Medical College, Baotou, ChinaSchool of Basic Medicine, Baotou Medical College, Baotou, China + Department of Clinical Laboratory, Shanghai University of Medicine & Health Sciences Affiliated Zhoupu Hospital, Shanghai, ChinaWhen used in ethnomedicine, Dracocephalum moldavica L. (DML) and its bioactive extract protect against several diseases caused by inflammation and oxidative stress. However, the effect of DML on intestinal inflammation in hypertension has not been thoroughly investigated. This study focused on the effects of DML extract on intestinal inflammation in two-kidney, one clip (2K1C) rats. Male Wistar rats were divided into three groups with daily intragastric administration of either saline (control group and model group) or DML extract (treatment group, 50 mg/kg per day) for 4 weeks. In the model and treatment groups, 2K1C hypertension was induced by clipping the left renal artery. The profiles of stool microbiota, the levels of short-chain fatty acids (SCFAs), the integrity of the gut barrier, oxidative stress biomarkers and inflammatory factors were analyzed in rats supplemented with or without DML extract. This study revealed the alleviation of high blood pressure in 2K1C rats treated with DML extract. The DML extract attenuated gut microbiota dysbiosis in the 2K1C rats by increasing the abundance of the genus Lactobacillus and by decreasing the abundance of Papillibacter, Desulfovibrio and Anaerobiospirillum genera. Treatment with the DML extract increased the levels of SCFAs and repaired the intestinal barrier, it reduced the release of oxidative stress biomarkers and inflammatory factors and suppressed the expression of TLR4 and NF-κB mRNAs. We conclude that the DML extract may alleviate intestinal inflammation by adjusting the profile of gut microbiota and enhancing the intestinal barrier.https://doiserbia.nb.rs/img/doi/0354-4664/2023/0354-46642300013Y.pdfhypertensiontwo-kidneyone clip ratsdracocephalum moldavica l.gut microbiotaintestinal inflammation |
| spellingShingle | Yu Hui Chen Zhangjun Chen Huixia Wang Zhanli Dracocephalum moldavica L. Extract ameliorates intestinal inflammation by regulating gut microbiota and repairing the intestinal barrier in 2k1c rats Archives of Biological Sciences hypertension two-kidney one clip rats dracocephalum moldavica l. gut microbiota intestinal inflammation |
| title | Dracocephalum moldavica L. Extract ameliorates intestinal inflammation by regulating gut microbiota and repairing the intestinal barrier in 2k1c rats |
| title_full | Dracocephalum moldavica L. Extract ameliorates intestinal inflammation by regulating gut microbiota and repairing the intestinal barrier in 2k1c rats |
| title_fullStr | Dracocephalum moldavica L. Extract ameliorates intestinal inflammation by regulating gut microbiota and repairing the intestinal barrier in 2k1c rats |
| title_full_unstemmed | Dracocephalum moldavica L. Extract ameliorates intestinal inflammation by regulating gut microbiota and repairing the intestinal barrier in 2k1c rats |
| title_short | Dracocephalum moldavica L. Extract ameliorates intestinal inflammation by regulating gut microbiota and repairing the intestinal barrier in 2k1c rats |
| title_sort | dracocephalum moldavica l extract ameliorates intestinal inflammation by regulating gut microbiota and repairing the intestinal barrier in 2k1c rats |
| topic | hypertension two-kidney one clip rats dracocephalum moldavica l. gut microbiota intestinal inflammation |
| url | https://doiserbia.nb.rs/img/doi/0354-4664/2023/0354-46642300013Y.pdf |
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