Hox genes polymorphism depicts developmental disruption of common sole eggs
In sole aquaculture production, consistency in the quality of produced eggs throughout the year is unpredictable. Hox genes have a crucial role in controlling embryonic development and their genetic variation could alter the phenotype dramatically. In teleosts genome duplication led paralog hox gene...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
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De Gruyter
2019-12-01
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| Series: | Open Life Sciences |
| Subjects: | |
| Online Access: | https://doi.org/10.1515/biol-2019-0061 |
| _version_ | 1819137346128314368 |
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| author | Kavouras Menelaos Malandrakis Emmanouil E. Danis Theodoros Blom Ewout Anastassiadis Konstantinos Panagiotaki Panagiota Exadactylos Athanasios |
| author_facet | Kavouras Menelaos Malandrakis Emmanouil E. Danis Theodoros Blom Ewout Anastassiadis Konstantinos Panagiotaki Panagiota Exadactylos Athanasios |
| author_sort | Kavouras Menelaos |
| collection | DOAJ |
| description | In sole aquaculture production, consistency in the quality of produced eggs throughout the year is unpredictable. Hox genes have a crucial role in controlling embryonic development and their genetic variation could alter the phenotype dramatically. In teleosts genome duplication led paralog hox genes to become diverged. Direct association of polymorphism in hoxa1a, hoxa2a & hoxa2b of Solea solea with egg viability indicates hoxa2b as a potential genetic marker. High Resolution Melt (HRM) analysis was carried out in 52 viable and 61 non-viable eggs collected at 54±6 hours post fertilization (hpf). Allelic and genotypic frequencies of polymorphism were analyzed and results illustrated a significantly increased risk for non-viability for minor alleles and their homozygous genotypes. Haplotype analysis demonstrated a significant recessive effect on the risk of non-viability, by increasing the odds of disrupting embryonic development up to three-fold. Phylogenetic analysis showed that the paralog genes hoxa2a and hoxa2b, are separated distinctly in two clades and presented a significant ω variation, revealing their diverged evolutionary rate. |
| first_indexed | 2024-12-22T10:49:24Z |
| format | Article |
| id | doaj.art-a31986dd227f40938358104d13eb9d82 |
| institution | Directory Open Access Journal |
| issn | 2391-5412 |
| language | English |
| last_indexed | 2024-12-22T10:49:24Z |
| publishDate | 2019-12-01 |
| publisher | De Gruyter |
| record_format | Article |
| series | Open Life Sciences |
| spelling | doaj.art-a31986dd227f40938358104d13eb9d822022-12-21T18:28:49ZengDe GruyterOpen Life Sciences2391-54122019-12-0114154956310.1515/biol-2019-0061biol-2019-0061Hox genes polymorphism depicts developmental disruption of common sole eggsKavouras Menelaos0Malandrakis Emmanouil E.1Danis Theodoros2Blom Ewout3Anastassiadis Konstantinos4Panagiotaki Panagiota5Exadactylos Athanasios6Fytokou str, 38446, Volos, GreeceDepartment of Ichthyology and Aquatic Environment, School of Agricultural Sciences, University of Thessaly, Fytokou str, Volos, GreeceDepartment of Ichthyology and Aquatic Environment, School of Agricultural Sciences, University of Thessaly, Fytokou str, Volos, GreeceWageningen Marine Research, Wageningen University & Research, IJmuiden, The NetherlandsBiotechnology Centre (BIOTEC), TU Dresden, Dresden, GermanyDepartment of Ichthyology and Aquatic Environment, School of Agricultural Sciences, University of Thessaly, Fytokou str, Volos, GreeceFytokou str, 38446, Volos, GreeceIn sole aquaculture production, consistency in the quality of produced eggs throughout the year is unpredictable. Hox genes have a crucial role in controlling embryonic development and their genetic variation could alter the phenotype dramatically. In teleosts genome duplication led paralog hox genes to become diverged. Direct association of polymorphism in hoxa1a, hoxa2a & hoxa2b of Solea solea with egg viability indicates hoxa2b as a potential genetic marker. High Resolution Melt (HRM) analysis was carried out in 52 viable and 61 non-viable eggs collected at 54±6 hours post fertilization (hpf). Allelic and genotypic frequencies of polymorphism were analyzed and results illustrated a significantly increased risk for non-viability for minor alleles and their homozygous genotypes. Haplotype analysis demonstrated a significant recessive effect on the risk of non-viability, by increasing the odds of disrupting embryonic development up to three-fold. Phylogenetic analysis showed that the paralog genes hoxa2a and hoxa2b, are separated distinctly in two clades and presented a significant ω variation, revealing their diverged evolutionary rate.https://doi.org/10.1515/biol-2019-0061egg qualityembryonic developmenthox genespolymorphismreproduction |
| spellingShingle | Kavouras Menelaos Malandrakis Emmanouil E. Danis Theodoros Blom Ewout Anastassiadis Konstantinos Panagiotaki Panagiota Exadactylos Athanasios Hox genes polymorphism depicts developmental disruption of common sole eggs Open Life Sciences egg quality embryonic development hox genes polymorphism reproduction |
| title | Hox genes polymorphism depicts developmental disruption of common sole eggs |
| title_full | Hox genes polymorphism depicts developmental disruption of common sole eggs |
| title_fullStr | Hox genes polymorphism depicts developmental disruption of common sole eggs |
| title_full_unstemmed | Hox genes polymorphism depicts developmental disruption of common sole eggs |
| title_short | Hox genes polymorphism depicts developmental disruption of common sole eggs |
| title_sort | hox genes polymorphism depicts developmental disruption of common sole eggs |
| topic | egg quality embryonic development hox genes polymorphism reproduction |
| url | https://doi.org/10.1515/biol-2019-0061 |
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