Characterization and Biotechnological Potential of Extracellular Polysaccharides Synthesized by <i>Alteromonas</i> Strains Isolated from French Polynesia Marine Environments
Marine environments comprise almost three quarters of Earth’s surface, representing the largest ecosystem of our planet. The vast ecological and metabolic diversity found in marine microorganisms suggest that these marine resources have a huge potential as sources of novel commercially appealing bio...
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| Format: | Article |
| Language: | English |
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MDPI AG
2021-09-01
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| Series: | Marine Drugs |
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| Online Access: | https://www.mdpi.com/1660-3397/19/9/522 |
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| author | Patrícia Concórdio-Reis Vítor D. Alves Xavier Moppert Jean Guézennec Filomena Freitas Maria A. M. Reis |
| author_facet | Patrícia Concórdio-Reis Vítor D. Alves Xavier Moppert Jean Guézennec Filomena Freitas Maria A. M. Reis |
| author_sort | Patrícia Concórdio-Reis |
| collection | DOAJ |
| description | Marine environments comprise almost three quarters of Earth’s surface, representing the largest ecosystem of our planet. The vast ecological and metabolic diversity found in marine microorganisms suggest that these marine resources have a huge potential as sources of novel commercially appealing biomolecules, such as exopolysaccharides (EPS). Six <i>Alteromonas</i> strains from different marine environments in French Polynesia atolls were selected for EPS extraction. All the EPS were heteropolysaccharides composed of different monomers, including neutral monosaccharides (glucose, galactose, and mannose, rhamnose and fucose), and uronic acids (glucuronic acid and galacturonic acid), which accounted for up to 45.5 mol% of the EPS compositions. Non-carbohydrate substituents, such as acetyl (0.5–2.1 wt%), pyruvyl (0.2–4.9 wt%), succinyl (1–1.8 wt%), and sulfate (1.98–3.43 wt%); and few peptides (1.72–6.77 wt%) were also detected. Thermal analysis demonstrated that the EPS had a degradation temperature above 260 °C, and high char yields (32–53%). Studies on EPS functional properties revealed that they produce viscous aqueous solutions with a shear thinning behavior and could form strong gels in two distinct ways: by the addition of Fe<sup>2+</sup>, or in the presence of Mg<sup>2+</sup>, Cu<sup>2+</sup>, or Ca<sup>2+</sup> under alkaline conditions. Thus, these EPS could be versatile materials for different applications. |
| first_indexed | 2024-03-10T07:30:11Z |
| format | Article |
| id | doaj.art-a31bd53277574d43b4fd77893b6450eb |
| institution | Directory Open Access Journal |
| issn | 1660-3397 |
| language | English |
| last_indexed | 2024-03-10T07:30:11Z |
| publishDate | 2021-09-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Marine Drugs |
| spelling | doaj.art-a31bd53277574d43b4fd77893b6450eb2023-11-22T13:59:03ZengMDPI AGMarine Drugs1660-33972021-09-0119952210.3390/md19090522Characterization and Biotechnological Potential of Extracellular Polysaccharides Synthesized by <i>Alteromonas</i> Strains Isolated from French Polynesia Marine EnvironmentsPatrícia Concórdio-Reis0Vítor D. Alves1Xavier Moppert2Jean Guézennec3Filomena Freitas4Maria A. M. Reis5Associate Laboratory i4HB–Institute for Health and Bioeconomy, School of Science and Technology, NOVA University Lisbon, 2829-516 Caparica, PortugalLEAF—Linking Landscape, Environment, Agriculture and Food—Research Center, Instituto Superior de Agronomia, Universidade de Lisboa, 1349-017 Lisbon, PortugalPacific Biotech SAS, BP 140 289, 98 701 Arue, Tahiti, French PolynesiaAiMB (Advices in Marine Biotechnology), 17 Rue d’Ouessant, 29280 Plouzané, FranceAssociate Laboratory i4HB–Institute for Health and Bioeconomy, School of Science and Technology, NOVA University Lisbon, 2829-516 Caparica, PortugalAssociate Laboratory i4HB–Institute for Health and Bioeconomy, School of Science and Technology, NOVA University Lisbon, 2829-516 Caparica, PortugalMarine environments comprise almost three quarters of Earth’s surface, representing the largest ecosystem of our planet. The vast ecological and metabolic diversity found in marine microorganisms suggest that these marine resources have a huge potential as sources of novel commercially appealing biomolecules, such as exopolysaccharides (EPS). Six <i>Alteromonas</i> strains from different marine environments in French Polynesia atolls were selected for EPS extraction. All the EPS were heteropolysaccharides composed of different monomers, including neutral monosaccharides (glucose, galactose, and mannose, rhamnose and fucose), and uronic acids (glucuronic acid and galacturonic acid), which accounted for up to 45.5 mol% of the EPS compositions. Non-carbohydrate substituents, such as acetyl (0.5–2.1 wt%), pyruvyl (0.2–4.9 wt%), succinyl (1–1.8 wt%), and sulfate (1.98–3.43 wt%); and few peptides (1.72–6.77 wt%) were also detected. Thermal analysis demonstrated that the EPS had a degradation temperature above 260 °C, and high char yields (32–53%). Studies on EPS functional properties revealed that they produce viscous aqueous solutions with a shear thinning behavior and could form strong gels in two distinct ways: by the addition of Fe<sup>2+</sup>, or in the presence of Mg<sup>2+</sup>, Cu<sup>2+</sup>, or Ca<sup>2+</sup> under alkaline conditions. Thus, these EPS could be versatile materials for different applications.https://www.mdpi.com/1660-3397/19/9/522<i>Alteromonas</i> sp.exopolysaccharide characterizationfunctional propertiesrheologygelation |
| spellingShingle | Patrícia Concórdio-Reis Vítor D. Alves Xavier Moppert Jean Guézennec Filomena Freitas Maria A. M. Reis Characterization and Biotechnological Potential of Extracellular Polysaccharides Synthesized by <i>Alteromonas</i> Strains Isolated from French Polynesia Marine Environments Marine Drugs <i>Alteromonas</i> sp. exopolysaccharide characterization functional properties rheology gelation |
| title | Characterization and Biotechnological Potential of Extracellular Polysaccharides Synthesized by <i>Alteromonas</i> Strains Isolated from French Polynesia Marine Environments |
| title_full | Characterization and Biotechnological Potential of Extracellular Polysaccharides Synthesized by <i>Alteromonas</i> Strains Isolated from French Polynesia Marine Environments |
| title_fullStr | Characterization and Biotechnological Potential of Extracellular Polysaccharides Synthesized by <i>Alteromonas</i> Strains Isolated from French Polynesia Marine Environments |
| title_full_unstemmed | Characterization and Biotechnological Potential of Extracellular Polysaccharides Synthesized by <i>Alteromonas</i> Strains Isolated from French Polynesia Marine Environments |
| title_short | Characterization and Biotechnological Potential of Extracellular Polysaccharides Synthesized by <i>Alteromonas</i> Strains Isolated from French Polynesia Marine Environments |
| title_sort | characterization and biotechnological potential of extracellular polysaccharides synthesized by i alteromonas i strains isolated from french polynesia marine environments |
| topic | <i>Alteromonas</i> sp. exopolysaccharide characterization functional properties rheology gelation |
| url | https://www.mdpi.com/1660-3397/19/9/522 |
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