High miRNA-378 expression has high diagnostic values for pulmonary tuberculosis and predicts adverse outcomes
Abstract Background Pulmonary tuberculosis (TB) is a chronic infectious disease. microRNA (miR)-378 is involved in TB diagnosis. This study explored the effects of miR-378 on TB patients. Methods A total of 126 TB patients were selected, including 63 active TB and 63 latent TB, with 62 healthy subje...
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| Format: | Article |
| Language: | English |
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BMC
2022-03-01
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| Series: | BMC Molecular and Cell Biology |
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| Online Access: | https://doi.org/10.1186/s12860-022-00413-w |
| _version_ | 1818646805473132544 |
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| author | Xiaolu Sun Kai Liu Yan Zhao Tianhua Zhang |
| author_facet | Xiaolu Sun Kai Liu Yan Zhao Tianhua Zhang |
| author_sort | Xiaolu Sun |
| collection | DOAJ |
| description | Abstract Background Pulmonary tuberculosis (TB) is a chronic infectious disease. microRNA (miR)-378 is involved in TB diagnosis. This study explored the effects of miR-378 on TB patients. Methods A total of 126 TB patients were selected, including 63 active TB and 63 latent TB, with 62 healthy subjects as controls. Serum miR-378 expression was detected. The diagnostic value of miR-378 in TB was analyzed using the ROC curve. Immune inflammatory factor levels were detected and their correlations with miR-378 expression were analyzed. The drug resistance of active TB patients was recorded after standard treatment. miR-378 expression in drug-resistant TB patients was detected. The effects of miR-378 on adverse outcome incidence were analyzed. Results miR-378 expression was highly expressed in TB and the expression was higher in the active group than the latent group. Serum miR-378 expression > 1.490 had high sensitivity and specificity in TB diagnosis. miR-378 expression was correlated with TB clinical indexes. IL-4, IL-6, and IL-1β levels were highly expressed, while IFN-γ, TNF-α, and IL-12 levels were lowly expressed in TB patients. Serum miR-378 level in the active group was positively correlated with serum IL-4, IL-6, and IL-1β, and negatively correlated with serum IFN-γ, TNF-α, and IL-12 concentrations. miR-378 expression was downregulated in the TB treated, single (SDR TB) and multi-drug resistance (MDR TB) groups, the miR-378 expression in SDR TB and MDR TB groups was higher than the TB treated group and lower in the SDR TB group than the MDR TB group. High miR-378 expression predicted higher adverse outcome incidence. Conclusions High miR-378 expression assisted TB diagnosis and predicted adverse outcomes. |
| first_indexed | 2024-12-17T00:52:28Z |
| format | Article |
| id | doaj.art-a31c25f33d8042f89775010ab5299207 |
| institution | Directory Open Access Journal |
| issn | 2661-8850 |
| language | English |
| last_indexed | 2024-12-17T00:52:28Z |
| publishDate | 2022-03-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Molecular and Cell Biology |
| spelling | doaj.art-a31c25f33d8042f89775010ab52992072022-12-21T22:09:44ZengBMCBMC Molecular and Cell Biology2661-88502022-03-0123111110.1186/s12860-022-00413-wHigh miRNA-378 expression has high diagnostic values for pulmonary tuberculosis and predicts adverse outcomesXiaolu Sun0Kai Liu1Yan Zhao2Tianhua Zhang3Department of Tuberculosis Treatmen and Prevention, Shaanxi Provincial Institute for Tuberculosis Control and PreventionDepartment of Orthopedics, First Affiliated Hospital of Xi’an Jiaotong UniversityDepartment of Tuberculosis Treatmen and Prevention, Shaanxi Provincial Institute for Tuberculosis Control and PreventionDepartment of Tuberculosis Treatmen and Prevention, Shaanxi Provincial Institute for Tuberculosis Control and PreventionAbstract Background Pulmonary tuberculosis (TB) is a chronic infectious disease. microRNA (miR)-378 is involved in TB diagnosis. This study explored the effects of miR-378 on TB patients. Methods A total of 126 TB patients were selected, including 63 active TB and 63 latent TB, with 62 healthy subjects as controls. Serum miR-378 expression was detected. The diagnostic value of miR-378 in TB was analyzed using the ROC curve. Immune inflammatory factor levels were detected and their correlations with miR-378 expression were analyzed. The drug resistance of active TB patients was recorded after standard treatment. miR-378 expression in drug-resistant TB patients was detected. The effects of miR-378 on adverse outcome incidence were analyzed. Results miR-378 expression was highly expressed in TB and the expression was higher in the active group than the latent group. Serum miR-378 expression > 1.490 had high sensitivity and specificity in TB diagnosis. miR-378 expression was correlated with TB clinical indexes. IL-4, IL-6, and IL-1β levels were highly expressed, while IFN-γ, TNF-α, and IL-12 levels were lowly expressed in TB patients. Serum miR-378 level in the active group was positively correlated with serum IL-4, IL-6, and IL-1β, and negatively correlated with serum IFN-γ, TNF-α, and IL-12 concentrations. miR-378 expression was downregulated in the TB treated, single (SDR TB) and multi-drug resistance (MDR TB) groups, the miR-378 expression in SDR TB and MDR TB groups was higher than the TB treated group and lower in the SDR TB group than the MDR TB group. High miR-378 expression predicted higher adverse outcome incidence. Conclusions High miR-378 expression assisted TB diagnosis and predicted adverse outcomes.https://doi.org/10.1186/s12860-022-00413-wmiR-378Pulmonary tuberculosisDrug-resistant TBImmunological functionAdverse outcomesReceiver operating characteristic curve |
| spellingShingle | Xiaolu Sun Kai Liu Yan Zhao Tianhua Zhang High miRNA-378 expression has high diagnostic values for pulmonary tuberculosis and predicts adverse outcomes BMC Molecular and Cell Biology miR-378 Pulmonary tuberculosis Drug-resistant TB Immunological function Adverse outcomes Receiver operating characteristic curve |
| title | High miRNA-378 expression has high diagnostic values for pulmonary tuberculosis and predicts adverse outcomes |
| title_full | High miRNA-378 expression has high diagnostic values for pulmonary tuberculosis and predicts adverse outcomes |
| title_fullStr | High miRNA-378 expression has high diagnostic values for pulmonary tuberculosis and predicts adverse outcomes |
| title_full_unstemmed | High miRNA-378 expression has high diagnostic values for pulmonary tuberculosis and predicts adverse outcomes |
| title_short | High miRNA-378 expression has high diagnostic values for pulmonary tuberculosis and predicts adverse outcomes |
| title_sort | high mirna 378 expression has high diagnostic values for pulmonary tuberculosis and predicts adverse outcomes |
| topic | miR-378 Pulmonary tuberculosis Drug-resistant TB Immunological function Adverse outcomes Receiver operating characteristic curve |
| url | https://doi.org/10.1186/s12860-022-00413-w |
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