In Vitro Immunomodulatory Effect of R10 Fraction of Garlic on Viability and Production of TNF-? in CD8+ T Cells
Introduction & Objective: -cells, especially CD8+ T lymphocytes are the most important cells in anti-tumor response. Previously R10 fraction of garlic extract was reported as an immuno-modulator which induced an effective cellular immunity and Th1 responses. In this study the in vitro immunomodu...
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| Format: | Article |
| Language: | fas |
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Hamadan University of Medical Sciences
2014-03-01
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| Series: | پزشکی بالینی ابن سینا |
| Subjects: | |
| Online Access: | http://sjh.umsha.ac.ir/article-1-111-en.html |
| _version_ | 1831807883995512832 |
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| author | Tuba Ghazanfari Hosein Rashidi Shohreh Jalaei Pegah Alijani |
| author_facet | Tuba Ghazanfari Hosein Rashidi Shohreh Jalaei Pegah Alijani |
| author_sort | Tuba Ghazanfari |
| collection | DOAJ |
| description | Introduction & Objective: -cells, especially CD8+ T lymphocytes are the most important cells in anti-tumor response. Previously R10 fraction of garlic extract was reported as an immuno-modulator which induced an effective cellular immunity and Th1 responses. In this study the in vitro immunomodulatory effect of R10 on CD8+ T cells viability and production of TNF-α were evaluated. Materials & Methods: In this experimental study, using monoclonal antibodies attached to magnetic beads with isolating columns by magnetic bead method, CD8+ T cells from spleen cells of Balb/C mice were isolated. R10 fraction based on molecular weight was prepared using Ultra filtration. MTT assay was used to evaluate cell viability. TNF-α level was meas-ured in the supernatant of culture of CD8+ T cells by ELISA. Obtained data was compared and analyzed using Nonparametric Test and Keraskel & Wanny's Test tests.. Results: The findings indicate that all dilutions of R10 fraction increased cell viability of CD8+ T cells in comparison with the negative control group and in the presence of ConA with dilution of 1:50 of R10 fraction significantly increased cell viability of CD8+ T Cells compared to ConA alone. Secretion of TNF-α significantly increased by all dilutions of R10 fraction. Conclusion: These findings suggest that R10 fraction of garlic can be used as an Immuno-modulator drug candidate for induction of cellular Immunity in tumor therapy. |
| first_indexed | 2024-12-22T20:13:04Z |
| format | Article |
| id | doaj.art-a31ed5c2cfba4b6288abfcca004c17e3 |
| institution | Directory Open Access Journal |
| issn | 2588-722X 2588-7238 |
| language | fas |
| last_indexed | 2024-12-22T20:13:04Z |
| publishDate | 2014-03-01 |
| publisher | Hamadan University of Medical Sciences |
| record_format | Article |
| series | پزشکی بالینی ابن سینا |
| spelling | doaj.art-a31ed5c2cfba4b6288abfcca004c17e32022-12-21T18:14:01ZfasHamadan University of Medical Sciencesپزشکی بالینی ابن سینا2588-722X2588-72382014-03-01204273279In Vitro Immunomodulatory Effect of R10 Fraction of Garlic on Viability and Production of TNF-? in CD8+ T CellsTuba Ghazanfari0Hosein Rashidi1Shohreh Jalaei2Pegah Alijani3 Introduction & Objective: -cells, especially CD8+ T lymphocytes are the most important cells in anti-tumor response. Previously R10 fraction of garlic extract was reported as an immuno-modulator which induced an effective cellular immunity and Th1 responses. In this study the in vitro immunomodulatory effect of R10 on CD8+ T cells viability and production of TNF-α were evaluated. Materials & Methods: In this experimental study, using monoclonal antibodies attached to magnetic beads with isolating columns by magnetic bead method, CD8+ T cells from spleen cells of Balb/C mice were isolated. R10 fraction based on molecular weight was prepared using Ultra filtration. MTT assay was used to evaluate cell viability. TNF-α level was meas-ured in the supernatant of culture of CD8+ T cells by ELISA. Obtained data was compared and analyzed using Nonparametric Test and Keraskel & Wanny's Test tests.. Results: The findings indicate that all dilutions of R10 fraction increased cell viability of CD8+ T cells in comparison with the negative control group and in the presence of ConA with dilution of 1:50 of R10 fraction significantly increased cell viability of CD8+ T Cells compared to ConA alone. Secretion of TNF-α significantly increased by all dilutions of R10 fraction. Conclusion: These findings suggest that R10 fraction of garlic can be used as an Immuno-modulator drug candidate for induction of cellular Immunity in tumor therapy.http://sjh.umsha.ac.ir/article-1-111-en.htmlcd8+ t cellscytokinesgarlicr10 fractionviability |
| spellingShingle | Tuba Ghazanfari Hosein Rashidi Shohreh Jalaei Pegah Alijani In Vitro Immunomodulatory Effect of R10 Fraction of Garlic on Viability and Production of TNF-? in CD8+ T Cells پزشکی بالینی ابن سینا cd8+ t cells cytokines garlic r10 fraction viability |
| title | In Vitro Immunomodulatory Effect of R10 Fraction of Garlic on Viability and Production of TNF-? in CD8+ T Cells |
| title_full | In Vitro Immunomodulatory Effect of R10 Fraction of Garlic on Viability and Production of TNF-? in CD8+ T Cells |
| title_fullStr | In Vitro Immunomodulatory Effect of R10 Fraction of Garlic on Viability and Production of TNF-? in CD8+ T Cells |
| title_full_unstemmed | In Vitro Immunomodulatory Effect of R10 Fraction of Garlic on Viability and Production of TNF-? in CD8+ T Cells |
| title_short | In Vitro Immunomodulatory Effect of R10 Fraction of Garlic on Viability and Production of TNF-? in CD8+ T Cells |
| title_sort | in vitro immunomodulatory effect of r10 fraction of garlic on viability and production of tnf in cd8 t cells |
| topic | cd8+ t cells cytokines garlic r10 fraction viability |
| url | http://sjh.umsha.ac.ir/article-1-111-en.html |
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