Umbilical Cord Blood Cells Do Not Reduce Ventilation-Induced Lung Injury in Preterm Lambs
BackgroundPreterm infants often have immature lungs and, consequently, many require respiratory support at birth. However, respiratory support causes lung inflammation and injury, termed ventilation-induced lung injury (VILI). Umbilical cord blood (UCB) contains five cell types that have been shown...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2020-02-01
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| Series: | Frontiers in Physiology |
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| Online Access: | https://www.frontiersin.org/article/10.3389/fphys.2020.00119/full |
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| author | Madeleine J. Smith Kyra Y. Y. Chan Paris Papagianis Paris Papagianis Ilias Nitsos Valerie Zahra Beth Allison Graeme R. Polglase Courtney A. McDonald |
| author_facet | Madeleine J. Smith Kyra Y. Y. Chan Paris Papagianis Paris Papagianis Ilias Nitsos Valerie Zahra Beth Allison Graeme R. Polglase Courtney A. McDonald |
| author_sort | Madeleine J. Smith |
| collection | DOAJ |
| description | BackgroundPreterm infants often have immature lungs and, consequently, many require respiratory support at birth. However, respiratory support causes lung inflammation and injury, termed ventilation-induced lung injury (VILI). Umbilical cord blood (UCB) contains five cell types that have been shown to reduce inflammation and injury. The aim of this study was to determine whether UCB cells can reduce VILI in preterm lambs.MethodsWe assessed lung inflammation and injury, with and without UCB cell administration. Fetal lambs at 125 ± 1 days gestation underwent sterile surgery and were randomly allocated to one of four groups; unoperated controls (UNOP), sham controls (SHAM), injuriously ventilated lambs (VILI), and injuriously ventilated lambs that received UCB cells via the jugular vein 1 h after ventilation (VILICELLS). Ventilated lambs received an injurious ventilation strategy for 15 min, before they were returned to the uterus and the lamb and ewe recovered for 24 h. After 24 h, lambs were delivered via caesarean section and euthanized and the lungs were collected for histological and molecular assessment of inflammation and injury.ResultsVILI led to increased immune cell infiltration, increased cellular proliferation, increased tissue wall thickness, and significantly reduced alveolar septation compared to controls. Further, extracellular matrix proteins collagen and elastin had abnormal deposition following VILI compared to control groups. Administration of UCB cells did not reduce any of these indices.ConclusionAdministration of UCB cells 1 h after ventilation onset did not reduce VILI in preterm lambs. |
| first_indexed | 2024-12-23T06:47:28Z |
| format | Article |
| id | doaj.art-a31f295c4738444b829e3095a1c6e26a |
| institution | Directory Open Access Journal |
| issn | 1664-042X |
| language | English |
| last_indexed | 2024-12-23T06:47:28Z |
| publishDate | 2020-02-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Physiology |
| spelling | doaj.art-a31f295c4738444b829e3095a1c6e26a2022-12-21T17:56:31ZengFrontiers Media S.A.Frontiers in Physiology1664-042X2020-02-011110.3389/fphys.2020.00119506781Umbilical Cord Blood Cells Do Not Reduce Ventilation-Induced Lung Injury in Preterm LambsMadeleine J. Smith0Kyra Y. Y. Chan1Paris Papagianis2Paris Papagianis3Ilias Nitsos4Valerie Zahra5Beth Allison6Graeme R. Polglase7Courtney A. McDonald8The Ritchie Centre, Department of Obstetrics and Gynaecology, Hudson Institute of Medical Research, Monash University, Melbourne, VIC, AustraliaThe Ritchie Centre, Department of Obstetrics and Gynaecology, Hudson Institute of Medical Research, Monash University, Melbourne, VIC, AustraliaThe Ritchie Centre, Department of Obstetrics and Gynaecology, Hudson Institute of Medical Research, Monash University, Melbourne, VIC, AustraliaChronic Infectious and Inflammatory Diseases Research, School of Health and Biomedical Sciences, RMIT University, Bundoora, VIC, AustraliaThe Ritchie Centre, Department of Obstetrics and Gynaecology, Hudson Institute of Medical Research, Monash University, Melbourne, VIC, AustraliaThe Ritchie Centre, Department of Obstetrics and Gynaecology, Hudson Institute of Medical Research, Monash University, Melbourne, VIC, AustraliaThe Ritchie Centre, Department of Obstetrics and Gynaecology, Hudson Institute of Medical Research, Monash University, Melbourne, VIC, AustraliaThe Ritchie Centre, Department of Obstetrics and Gynaecology, Hudson Institute of Medical Research, Monash University, Melbourne, VIC, AustraliaThe Ritchie Centre, Department of Obstetrics and Gynaecology, Hudson Institute of Medical Research, Monash University, Melbourne, VIC, AustraliaBackgroundPreterm infants often have immature lungs and, consequently, many require respiratory support at birth. However, respiratory support causes lung inflammation and injury, termed ventilation-induced lung injury (VILI). Umbilical cord blood (UCB) contains five cell types that have been shown to reduce inflammation and injury. The aim of this study was to determine whether UCB cells can reduce VILI in preterm lambs.MethodsWe assessed lung inflammation and injury, with and without UCB cell administration. Fetal lambs at 125 ± 1 days gestation underwent sterile surgery and were randomly allocated to one of four groups; unoperated controls (UNOP), sham controls (SHAM), injuriously ventilated lambs (VILI), and injuriously ventilated lambs that received UCB cells via the jugular vein 1 h after ventilation (VILICELLS). Ventilated lambs received an injurious ventilation strategy for 15 min, before they were returned to the uterus and the lamb and ewe recovered for 24 h. After 24 h, lambs were delivered via caesarean section and euthanized and the lungs were collected for histological and molecular assessment of inflammation and injury.ResultsVILI led to increased immune cell infiltration, increased cellular proliferation, increased tissue wall thickness, and significantly reduced alveolar septation compared to controls. Further, extracellular matrix proteins collagen and elastin had abnormal deposition following VILI compared to control groups. Administration of UCB cells did not reduce any of these indices.ConclusionAdministration of UCB cells 1 h after ventilation onset did not reduce VILI in preterm lambs.https://www.frontiersin.org/article/10.3389/fphys.2020.00119/fulllungventilation-induced lung injurybronchopulmonary dysplasiapretermumbilical cord blood cellsinflammation |
| spellingShingle | Madeleine J. Smith Kyra Y. Y. Chan Paris Papagianis Paris Papagianis Ilias Nitsos Valerie Zahra Beth Allison Graeme R. Polglase Courtney A. McDonald Umbilical Cord Blood Cells Do Not Reduce Ventilation-Induced Lung Injury in Preterm Lambs Frontiers in Physiology lung ventilation-induced lung injury bronchopulmonary dysplasia preterm umbilical cord blood cells inflammation |
| title | Umbilical Cord Blood Cells Do Not Reduce Ventilation-Induced Lung Injury in Preterm Lambs |
| title_full | Umbilical Cord Blood Cells Do Not Reduce Ventilation-Induced Lung Injury in Preterm Lambs |
| title_fullStr | Umbilical Cord Blood Cells Do Not Reduce Ventilation-Induced Lung Injury in Preterm Lambs |
| title_full_unstemmed | Umbilical Cord Blood Cells Do Not Reduce Ventilation-Induced Lung Injury in Preterm Lambs |
| title_short | Umbilical Cord Blood Cells Do Not Reduce Ventilation-Induced Lung Injury in Preterm Lambs |
| title_sort | umbilical cord blood cells do not reduce ventilation induced lung injury in preterm lambs |
| topic | lung ventilation-induced lung injury bronchopulmonary dysplasia preterm umbilical cord blood cells inflammation |
| url | https://www.frontiersin.org/article/10.3389/fphys.2020.00119/full |
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