Spread of ST348 <i>Klebsiella pneumoniae</i> Producing NDM-1 in a Peruvian Hospital
The aim of this study was to characterize carbapenem-resistant <i>Klebsiella pneumoniae</i> (CR-Kp) isolates recovered from adults and children with severe bacteremia in a Peruvian Hospital in June 2018. Antimicrobial susceptibility was determined by disc/gradient diffusion and broth mic...
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MDPI AG
2020-09-01
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| Series: | Microorganisms |
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| Online Access: | https://www.mdpi.com/2076-2607/8/9/1392 |
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| author | Maria J. Pons Marta Marí-Almirall Barbara Ymaña Jeel Moya-Salazar Laura Muñoz Sharon Sauñe Richard Salazar-Hernández Jordi Vila Ignasi Roca |
| author_facet | Maria J. Pons Marta Marí-Almirall Barbara Ymaña Jeel Moya-Salazar Laura Muñoz Sharon Sauñe Richard Salazar-Hernández Jordi Vila Ignasi Roca |
| author_sort | Maria J. Pons |
| collection | DOAJ |
| description | The aim of this study was to characterize carbapenem-resistant <i>Klebsiella pneumoniae</i> (CR-Kp) isolates recovered from adults and children with severe bacteremia in a Peruvian Hospital in June 2018. Antimicrobial susceptibility was determined by disc/gradient diffusion and broth microdilution when necessary. Antibiotic resistance mechanisms were evaluated by PCR and DNA sequencing. Clonal relatedness was assessed using pulsed-field gel electrophoresis (PFGE) and multilocus sequence typing (MLST). Plasmid typing was performed with a PCR-based method. Thirty CR-Kp isolates were recovered in June 2018. All isolates were non-susceptible to all β-lactams, ciprofloxacin, gentamicin and trimethoprim-sulfamethoxazole, while mostly remaining susceptible to colistin, tigecycline, levofloxacin and amikacin. All isolates carried the <i>bla</i><sub>NDM-1</sub> gene and were extended spectrum β-lactamase (ESBL) producers. PFGE showed four different pulsotypes although all isolates but two belonged to the ST348 sequence type, previously reported in Portugal. <i>bla</i><sub>NDM-1</sub> was located in an IncFIB-M conjugative plasmid. To our knowledge, this is the first report of an New Delhi metallo-β-lactamase (NDM)-producing <i>K. pneumoniae</i> recovered from both children and adults in Lima, Peru, as well as the first time that the outbreak strain ST348 is reported in Peru and is associated with NDM. Studies providing epidemiological and molecular data on CR-Kp in Peru are essential to monitor their dissemination and prevent further spread. |
| first_indexed | 2024-03-10T16:24:40Z |
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| institution | Directory Open Access Journal |
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| language | English |
| last_indexed | 2024-03-10T16:24:40Z |
| publishDate | 2020-09-01 |
| publisher | MDPI AG |
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| series | Microorganisms |
| spelling | doaj.art-a3221720050741ff8e9cf1c7fe90c91e2023-11-20T13:20:22ZengMDPI AGMicroorganisms2076-26072020-09-0189139210.3390/microorganisms8091392Spread of ST348 <i>Klebsiella pneumoniae</i> Producing NDM-1 in a Peruvian HospitalMaria J. Pons0Marta Marí-Almirall1Barbara Ymaña2Jeel Moya-Salazar3Laura Muñoz4Sharon Sauñe5Richard Salazar-Hernández6Jordi Vila7Ignasi Roca8Facultad de Medicina, Universidad Científica del Sur, Carr. Antigua Panamericana Sur 19, Villa El Salvador, Lima 15067, PeruDepartment of Clinical Microbiology, ISGlobal, Hospital Clínic, Universitat de Barcelona, Rosselló 149-153, 08036 Barcelona, SpainFacultad de Medicina, Universidad Científica del Sur, Carr. Antigua Panamericana Sur 19, Villa El Salvador, Lima 15067, PeruHospital Nacional Docente Madre Niño San Bartolomé, Avenida Alfonso Ugarte 825, Lima 150101, PeruDepartment of Clinical Microbiology, ISGlobal, Hospital Clínic, Universitat de Barcelona, Rosselló 149-153, 08036 Barcelona, SpainEscuela de Posgrado, Facultad de Ciencias de la Salud, Universidad Privada San Juan Bautista, Ex Hacienda Villa, Av José Antonio Lavalle s/n, Lima 15067, PeruServicio de Citología y Citogenética, Departamento de Anatomía Patológica, Hospital Nacional Guillermo Almenara Irigoyen, Jirón García Naranjo 840, La Victoria 13, Lima 150115, PeruDepartment of Clinical Microbiology, ISGlobal, Hospital Clínic, Universitat de Barcelona, Rosselló 149-153, 08036 Barcelona, SpainDepartment of Clinical Microbiology, ISGlobal, Hospital Clínic, Universitat de Barcelona, Rosselló 149-153, 08036 Barcelona, SpainThe aim of this study was to characterize carbapenem-resistant <i>Klebsiella pneumoniae</i> (CR-Kp) isolates recovered from adults and children with severe bacteremia in a Peruvian Hospital in June 2018. Antimicrobial susceptibility was determined by disc/gradient diffusion and broth microdilution when necessary. Antibiotic resistance mechanisms were evaluated by PCR and DNA sequencing. Clonal relatedness was assessed using pulsed-field gel electrophoresis (PFGE) and multilocus sequence typing (MLST). Plasmid typing was performed with a PCR-based method. Thirty CR-Kp isolates were recovered in June 2018. All isolates were non-susceptible to all β-lactams, ciprofloxacin, gentamicin and trimethoprim-sulfamethoxazole, while mostly remaining susceptible to colistin, tigecycline, levofloxacin and amikacin. All isolates carried the <i>bla</i><sub>NDM-1</sub> gene and were extended spectrum β-lactamase (ESBL) producers. PFGE showed four different pulsotypes although all isolates but two belonged to the ST348 sequence type, previously reported in Portugal. <i>bla</i><sub>NDM-1</sub> was located in an IncFIB-M conjugative plasmid. To our knowledge, this is the first report of an New Delhi metallo-β-lactamase (NDM)-producing <i>K. pneumoniae</i> recovered from both children and adults in Lima, Peru, as well as the first time that the outbreak strain ST348 is reported in Peru and is associated with NDM. Studies providing epidemiological and molecular data on CR-Kp in Peru are essential to monitor their dissemination and prevent further spread.https://www.mdpi.com/2076-2607/8/9/1392carbapenem resistancemetallo-β-lactamase<i>Klebsiella pneumoniae</i>epidemiologybloodstream infections |
| spellingShingle | Maria J. Pons Marta Marí-Almirall Barbara Ymaña Jeel Moya-Salazar Laura Muñoz Sharon Sauñe Richard Salazar-Hernández Jordi Vila Ignasi Roca Spread of ST348 <i>Klebsiella pneumoniae</i> Producing NDM-1 in a Peruvian Hospital Microorganisms carbapenem resistance metallo-β-lactamase <i>Klebsiella pneumoniae</i> epidemiology bloodstream infections |
| title | Spread of ST348 <i>Klebsiella pneumoniae</i> Producing NDM-1 in a Peruvian Hospital |
| title_full | Spread of ST348 <i>Klebsiella pneumoniae</i> Producing NDM-1 in a Peruvian Hospital |
| title_fullStr | Spread of ST348 <i>Klebsiella pneumoniae</i> Producing NDM-1 in a Peruvian Hospital |
| title_full_unstemmed | Spread of ST348 <i>Klebsiella pneumoniae</i> Producing NDM-1 in a Peruvian Hospital |
| title_short | Spread of ST348 <i>Klebsiella pneumoniae</i> Producing NDM-1 in a Peruvian Hospital |
| title_sort | spread of st348 i klebsiella pneumoniae i producing ndm 1 in a peruvian hospital |
| topic | carbapenem resistance metallo-β-lactamase <i>Klebsiella pneumoniae</i> epidemiology bloodstream infections |
| url | https://www.mdpi.com/2076-2607/8/9/1392 |
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