Antimicrobial Coating Efficacy for Prevention of Pseudomonas aeruginosa Biofilm Growth on ISS Water System Materials
Biofilms can lead to biofouling, microbially induced corrosion, physical impediment and eventual loss in function of water systems, and other engineered systems. The remoteness and closed environment of the International Space Station (ISS) make it vulnerable to unchecked biofilm growth; thus, biofi...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2022-04-01
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| Series: | Frontiers in Microbiology |
| Subjects: | |
| Online Access: | https://www.frontiersin.org/articles/10.3389/fmicb.2022.874236/full |
| _version_ | 1811313099373805568 |
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| author | Madelyn K. Mettler Ceth W. Parker Kasthuri Venkateswaran Brent M. Peyton |
| author_facet | Madelyn K. Mettler Ceth W. Parker Kasthuri Venkateswaran Brent M. Peyton |
| author_sort | Madelyn K. Mettler |
| collection | DOAJ |
| description | Biofilms can lead to biofouling, microbially induced corrosion, physical impediment and eventual loss in function of water systems, and other engineered systems. The remoteness and closed environment of the International Space Station (ISS) make it vulnerable to unchecked biofilm growth; thus, biofilm mitigation strategies are crucial for current ISS operation and future long duration and deep-space crewed missions. In this study, a space flown bacterial strain of Pseudomonas aeruginosa (PA14) was used as a model organism for its ability to form biofilms. Additionally, a novel antimicrobial coating’s ability to reduce biofilm accumulation on stainless steel, Teflon, titanium, and Inconel (all used in the ISS water treatment and handling systems) was analyzed. Coated materials demonstrated reductions of P. aeruginosa biofilm across all materials when tested in a continuous flow system with tryptic soy broth medium. However, the coating lost efficacy in potato dextrose broth medium. These findings were corroborated via scanning electron microscopy. This study illustrates the fundamental importance of using multiple approaches to test antibiofilm strategies, as well as the specificity in which conditions such strategies can be implemented. |
| first_indexed | 2024-04-13T10:49:25Z |
| format | Article |
| id | doaj.art-a322e02c93d24ef6924558db46c1d6fc |
| institution | Directory Open Access Journal |
| issn | 1664-302X |
| language | English |
| last_indexed | 2024-04-13T10:49:25Z |
| publishDate | 2022-04-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Microbiology |
| spelling | doaj.art-a322e02c93d24ef6924558db46c1d6fc2022-12-22T02:49:43ZengFrontiers Media S.A.Frontiers in Microbiology1664-302X2022-04-011310.3389/fmicb.2022.874236874236Antimicrobial Coating Efficacy for Prevention of Pseudomonas aeruginosa Biofilm Growth on ISS Water System MaterialsMadelyn K. Mettler0Ceth W. Parker1Kasthuri Venkateswaran2Brent M. Peyton3Center for Biofilm Engineering, Montana State University, Bozeman, MT, United StatesNASA Jet Propulsion Laboratory, California Institute of Technology, Pasadena, CA, United StatesNASA Jet Propulsion Laboratory, California Institute of Technology, Pasadena, CA, United StatesCenter for Biofilm Engineering, Montana State University, Bozeman, MT, United StatesBiofilms can lead to biofouling, microbially induced corrosion, physical impediment and eventual loss in function of water systems, and other engineered systems. The remoteness and closed environment of the International Space Station (ISS) make it vulnerable to unchecked biofilm growth; thus, biofilm mitigation strategies are crucial for current ISS operation and future long duration and deep-space crewed missions. In this study, a space flown bacterial strain of Pseudomonas aeruginosa (PA14) was used as a model organism for its ability to form biofilms. Additionally, a novel antimicrobial coating’s ability to reduce biofilm accumulation on stainless steel, Teflon, titanium, and Inconel (all used in the ISS water treatment and handling systems) was analyzed. Coated materials demonstrated reductions of P. aeruginosa biofilm across all materials when tested in a continuous flow system with tryptic soy broth medium. However, the coating lost efficacy in potato dextrose broth medium. These findings were corroborated via scanning electron microscopy. This study illustrates the fundamental importance of using multiple approaches to test antibiofilm strategies, as well as the specificity in which conditions such strategies can be implemented.https://www.frontiersin.org/articles/10.3389/fmicb.2022.874236/fullbiofilmPseudomonas aeruginosa PA14antimicrobialInternational Space StationNASAbuilt environment |
| spellingShingle | Madelyn K. Mettler Ceth W. Parker Kasthuri Venkateswaran Brent M. Peyton Antimicrobial Coating Efficacy for Prevention of Pseudomonas aeruginosa Biofilm Growth on ISS Water System Materials Frontiers in Microbiology biofilm Pseudomonas aeruginosa PA14 antimicrobial International Space Station NASA built environment |
| title | Antimicrobial Coating Efficacy for Prevention of Pseudomonas aeruginosa Biofilm Growth on ISS Water System Materials |
| title_full | Antimicrobial Coating Efficacy for Prevention of Pseudomonas aeruginosa Biofilm Growth on ISS Water System Materials |
| title_fullStr | Antimicrobial Coating Efficacy for Prevention of Pseudomonas aeruginosa Biofilm Growth on ISS Water System Materials |
| title_full_unstemmed | Antimicrobial Coating Efficacy for Prevention of Pseudomonas aeruginosa Biofilm Growth on ISS Water System Materials |
| title_short | Antimicrobial Coating Efficacy for Prevention of Pseudomonas aeruginosa Biofilm Growth on ISS Water System Materials |
| title_sort | antimicrobial coating efficacy for prevention of pseudomonas aeruginosa biofilm growth on iss water system materials |
| topic | biofilm Pseudomonas aeruginosa PA14 antimicrobial International Space Station NASA built environment |
| url | https://www.frontiersin.org/articles/10.3389/fmicb.2022.874236/full |
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