PHENOTYPIC AND MOLECULAR IDENTIFICATION OF YEASTS ISOLATED FROM CULTURED TILAPIA (Oreochromis niloticus) IN KAFRELSHEIKH PROVINCE, EGYPT
The present study aimed to identify yeasts by phenotypic, biochemical and genotypic methods. Three hundred live Oreochromis niloticus samples were collected from different locations at Kafrelsheikh Governorate, Egypt and during different seasons. Yeast isolates were identified according to their ph...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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University of Ljubljana Press (Založba Univerze v Ljubljani)
2019-03-01
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| Series: | Slovenian Veterinary Research |
| Online Access: | https://www.slovetres.si/index.php/SVR/article/view/803 |
| _version_ | 1797225024273252352 |
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| author | Amgad Moawad Ahmed Noor El Deen Nadia Mahfouz Salwa Helmy Yasmen Hashem |
| author_facet | Amgad Moawad Ahmed Noor El Deen Nadia Mahfouz Salwa Helmy Yasmen Hashem |
| author_sort | Amgad Moawad |
| collection | DOAJ |
| description |
The present study aimed to identify yeasts by phenotypic, biochemical and genotypic methods. Three hundred live Oreochromis niloticus samples were collected from different locations at Kafrelsheikh Governorate, Egypt and during different seasons. Yeast isolates were identified according to their phenotypical characterization in combination with polymerase chain reaction (PCR) and restriction fragment length polymorphism (RFLP) method. Two hundred and twenty-six yeast isolates from eighty diseased and two hundred and twenty apparently healthy O. niloticus samples. Isolated yeasts were Rhodotorula spp. (18.14%), Candida albicans (19.47%), Candida parapsilosis (16.37%), Candida guilliermondii (14.6%), Candida inconspicua (8.85%), Trichosporon asahii (17.25%), Geotrichum spp. (2.66%) and unidentified Ascus forming yeasts (2.66%). Yeasts most frequently isolated from gills and skin. Phenotypic methods are valuable in identification of yeasts into genera but these methods take more time. Molecular methods through the amplification of (internal transcribed spacer) ITS1-5.8S-ITS2 regions of fungal rRNA, followed by RFLP-PCR using Msp1 restriction enzyme allowed simple, rapid, cost-effective, sensetive and accurate identification of the phenotypically identified yeasts.
Key words: Oreochromis niloticus; yeast; PCR; Msp1; PCR-RFLP
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| first_indexed | 2024-04-24T14:02:26Z |
| format | Article |
| id | doaj.art-a32337c6ae8c468ebccb435854f37a11 |
| institution | Directory Open Access Journal |
| issn | 1580-4003 2385-8761 |
| language | English |
| last_indexed | 2024-04-24T14:02:26Z |
| publishDate | 2019-03-01 |
| publisher | University of Ljubljana Press (Založba Univerze v Ljubljani) |
| record_format | Article |
| series | Slovenian Veterinary Research |
| spelling | doaj.art-a32337c6ae8c468ebccb435854f37a112024-04-03T12:39:22ZengUniversity of Ljubljana Press (Založba Univerze v Ljubljani)Slovenian Veterinary Research1580-40032385-87612019-03-015622-Suppl10.26873/SVR-803-2019PHENOTYPIC AND MOLECULAR IDENTIFICATION OF YEASTS ISOLATED FROM CULTURED TILAPIA (Oreochromis niloticus) IN KAFRELSHEIKH PROVINCE, EGYPTAmgad MoawadAhmed Noor El DeenNadia MahfouzSalwa HelmyYasmen Hashem The present study aimed to identify yeasts by phenotypic, biochemical and genotypic methods. Three hundred live Oreochromis niloticus samples were collected from different locations at Kafrelsheikh Governorate, Egypt and during different seasons. Yeast isolates were identified according to their phenotypical characterization in combination with polymerase chain reaction (PCR) and restriction fragment length polymorphism (RFLP) method. Two hundred and twenty-six yeast isolates from eighty diseased and two hundred and twenty apparently healthy O. niloticus samples. Isolated yeasts were Rhodotorula spp. (18.14%), Candida albicans (19.47%), Candida parapsilosis (16.37%), Candida guilliermondii (14.6%), Candida inconspicua (8.85%), Trichosporon asahii (17.25%), Geotrichum spp. (2.66%) and unidentified Ascus forming yeasts (2.66%). Yeasts most frequently isolated from gills and skin. Phenotypic methods are valuable in identification of yeasts into genera but these methods take more time. Molecular methods through the amplification of (internal transcribed spacer) ITS1-5.8S-ITS2 regions of fungal rRNA, followed by RFLP-PCR using Msp1 restriction enzyme allowed simple, rapid, cost-effective, sensetive and accurate identification of the phenotypically identified yeasts. Key words: Oreochromis niloticus; yeast; PCR; Msp1; PCR-RFLP https://www.slovetres.si/index.php/SVR/article/view/803 |
| spellingShingle | Amgad Moawad Ahmed Noor El Deen Nadia Mahfouz Salwa Helmy Yasmen Hashem PHENOTYPIC AND MOLECULAR IDENTIFICATION OF YEASTS ISOLATED FROM CULTURED TILAPIA (Oreochromis niloticus) IN KAFRELSHEIKH PROVINCE, EGYPT Slovenian Veterinary Research |
| title | PHENOTYPIC AND MOLECULAR IDENTIFICATION OF YEASTS ISOLATED FROM CULTURED TILAPIA (Oreochromis niloticus) IN KAFRELSHEIKH PROVINCE, EGYPT |
| title_full | PHENOTYPIC AND MOLECULAR IDENTIFICATION OF YEASTS ISOLATED FROM CULTURED TILAPIA (Oreochromis niloticus) IN KAFRELSHEIKH PROVINCE, EGYPT |
| title_fullStr | PHENOTYPIC AND MOLECULAR IDENTIFICATION OF YEASTS ISOLATED FROM CULTURED TILAPIA (Oreochromis niloticus) IN KAFRELSHEIKH PROVINCE, EGYPT |
| title_full_unstemmed | PHENOTYPIC AND MOLECULAR IDENTIFICATION OF YEASTS ISOLATED FROM CULTURED TILAPIA (Oreochromis niloticus) IN KAFRELSHEIKH PROVINCE, EGYPT |
| title_short | PHENOTYPIC AND MOLECULAR IDENTIFICATION OF YEASTS ISOLATED FROM CULTURED TILAPIA (Oreochromis niloticus) IN KAFRELSHEIKH PROVINCE, EGYPT |
| title_sort | phenotypic and molecular identification of yeasts isolated from cultured tilapia oreochromis niloticus in kafrelsheikh province egypt |
| url | https://www.slovetres.si/index.php/SVR/article/view/803 |
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