Cytological and Proteomic Analysis of Ginkgo biloba Pollen Intine
The pollen intine plays important roles in pollen germination and tube growth, but related information in Ginkgo biloba remains unclear. We isolated and obtained de-exined pollen from G. biloba. Using fluorescent probes, we observed the strongest cellulose fluorescence in the pollen intine. De-ester...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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KeAi Communications Co., Ltd.
2020-07-01
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| Series: | Horticultural Plant Journal |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2468014120300388 |
| _version_ | 1797205236806320128 |
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| author | Weixing Li Yunling Ye Fangmei Cheng Yan Lu Biao Jin Li Wang |
| author_facet | Weixing Li Yunling Ye Fangmei Cheng Yan Lu Biao Jin Li Wang |
| author_sort | Weixing Li |
| collection | DOAJ |
| description | The pollen intine plays important roles in pollen germination and tube growth, but related information in Ginkgo biloba remains unclear. We isolated and obtained de-exined pollen from G. biloba. Using fluorescent probes, we observed the strongest cellulose fluorescence in the pollen intine. De-esterified pectin immunolabeled with JIM5 was present throughout the entire cell wall, whereas esterified pectin recognized by the monoclonal antibody JIM7 was concentrated in some regions. Callose staining with aniline blue was observed across the entire surface of the pollen intine. These results were confirmed by Fourier Transform InfraRed (FTIR) analysis. We also used proteomic approaches to identify different proteins between mature and de-exined pollen (48 h after hydration) in vitro. Based on mass spectrometry, de-exined pollen had more proteins than mature pollen, including calmodulin, serine hydroxymethyltransferase, β-galactosidase 6, and class IV chitinase. According to Gene Ontology (GO) analysis, the differentially expressed proteins were mainly associated with transportation, defense reaction, sugar metabolism, energy metabolism, signal transduction, and cell wall formation. These findings suggest that most proteins involved in pollen germination and pollen tube growth are synthesized during pollen hydration, indicating the important role of pollen hydration in the reproductive process of G. Biloba. |
| first_indexed | 2024-04-24T08:47:55Z |
| format | Article |
| id | doaj.art-a325908f859840a09936328fe988f399 |
| institution | Directory Open Access Journal |
| issn | 2468-0141 |
| language | English |
| last_indexed | 2024-04-24T08:47:55Z |
| publishDate | 2020-07-01 |
| publisher | KeAi Communications Co., Ltd. |
| record_format | Article |
| series | Horticultural Plant Journal |
| spelling | doaj.art-a325908f859840a09936328fe988f3992024-04-16T13:04:24ZengKeAi Communications Co., Ltd.Horticultural Plant Journal2468-01412020-07-0164257266Cytological and Proteomic Analysis of Ginkgo biloba Pollen IntineWeixing Li0Yunling Ye1Fangmei Cheng2Yan Lu3Biao Jin4Li Wang5College of Horticulture and Plant Protection, Yangzhou University, Yangzhou 225009, ChinaCollege of Horticulture and Plant Protection, Yangzhou University, Yangzhou 225009, ChinaSuqian Academy of Protected Horticulture, Nanjing Agricultural University, Suqian, Jiangsu 223800, ChinaInstrumental Analysis Center, Yangzhou University, Yangzhou 225009, ChinaCollege of Horticulture and Plant Protection, Yangzhou University, Yangzhou 225009, ChinaCollege of Horticulture and Plant Protection, Yangzhou University, Yangzhou 225009, China; Corresponding author. Tel: +86 514 87979395The pollen intine plays important roles in pollen germination and tube growth, but related information in Ginkgo biloba remains unclear. We isolated and obtained de-exined pollen from G. biloba. Using fluorescent probes, we observed the strongest cellulose fluorescence in the pollen intine. De-esterified pectin immunolabeled with JIM5 was present throughout the entire cell wall, whereas esterified pectin recognized by the monoclonal antibody JIM7 was concentrated in some regions. Callose staining with aniline blue was observed across the entire surface of the pollen intine. These results were confirmed by Fourier Transform InfraRed (FTIR) analysis. We also used proteomic approaches to identify different proteins between mature and de-exined pollen (48 h after hydration) in vitro. Based on mass spectrometry, de-exined pollen had more proteins than mature pollen, including calmodulin, serine hydroxymethyltransferase, β-galactosidase 6, and class IV chitinase. According to Gene Ontology (GO) analysis, the differentially expressed proteins were mainly associated with transportation, defense reaction, sugar metabolism, energy metabolism, signal transduction, and cell wall formation. These findings suggest that most proteins involved in pollen germination and pollen tube growth are synthesized during pollen hydration, indicating the important role of pollen hydration in the reproductive process of G. Biloba.http://www.sciencedirect.com/science/article/pii/S2468014120300388Ginkgo bilobapollen intinecytological analysisproteomicsmass spectrometry |
| spellingShingle | Weixing Li Yunling Ye Fangmei Cheng Yan Lu Biao Jin Li Wang Cytological and Proteomic Analysis of Ginkgo biloba Pollen Intine Horticultural Plant Journal Ginkgo biloba pollen intine cytological analysis proteomics mass spectrometry |
| title | Cytological and Proteomic Analysis of Ginkgo biloba Pollen Intine |
| title_full | Cytological and Proteomic Analysis of Ginkgo biloba Pollen Intine |
| title_fullStr | Cytological and Proteomic Analysis of Ginkgo biloba Pollen Intine |
| title_full_unstemmed | Cytological and Proteomic Analysis of Ginkgo biloba Pollen Intine |
| title_short | Cytological and Proteomic Analysis of Ginkgo biloba Pollen Intine |
| title_sort | cytological and proteomic analysis of ginkgo biloba pollen intine |
| topic | Ginkgo biloba pollen intine cytological analysis proteomics mass spectrometry |
| url | http://www.sciencedirect.com/science/article/pii/S2468014120300388 |
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