Cytotoxicity and Antimicrobial Activity of BioAkt<sup>TM</sup> and Phytic Acid: A Laboratory-Based Study
(1) Background: To improve endodontic treatments, it is necessary to find good irrigant solutions that balance potential toxic effects with optimal antimicrobial capacity. The aim of this laboratory study was to assess the cytotoxic and antimicrobial capacity of phytic acid (IP6) and BioAkt<sup&g...
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| Format: | Article |
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MDPI AG
2022-09-01
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| Series: | Applied Sciences |
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| Online Access: | https://www.mdpi.com/2076-3417/12/19/9715 |
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| author | Paolo Bertoletti Matteo Salvadori Riccardo Tonini Diletta Forgione Jacopo Francinelli Maria Luisa Garo Stefano Salgarello |
| author_facet | Paolo Bertoletti Matteo Salvadori Riccardo Tonini Diletta Forgione Jacopo Francinelli Maria Luisa Garo Stefano Salgarello |
| author_sort | Paolo Bertoletti |
| collection | DOAJ |
| description | (1) Background: To improve endodontic treatments, it is necessary to find good irrigant solutions that balance potential toxic effects with optimal antimicrobial capacity. The aim of this laboratory study was to assess the cytotoxic and antimicrobial capacity of phytic acid (IP6) and BioAkt<sup>TM</sup> in a laboratory setting to determine their suitability prior to endodontic evaluation. (2) Methods: The antimicrobial and cytotoxic effects of IP6 and BioAkt<sup>TM</sup> were compared with those of 1.5% NaOCl. Cytotoxicity was evaluated via crystal violet assay and CellTiter-Glo<sup>®</sup> 2.0 assay, while antimicrobial capacity was tested via disk diffusion and the effect of a 1-h treatment on Enterococcus faecalis. (3) Results: A crystal violet test and CellTiter-Glo<sup>®</sup> 2.0 assay showed statistically significant differences in cell viability and cell activity after 4 and 24 h for all tested solutions (<i>p</i> < 0.05). The antimicrobial activity of BioAkt<sup>TM</sup> was similar to that of 1.5% NaOCl, while phytic acid showed the lowest antimicrobial activity compared to BioAkt<sup>TM</sup> (<i>p</i> < 0.05) and 1.5% NaOCl (<i>p</i> < 0.05). (4) Conclusion: BioAkt<sup>TM</sup> showed an optimal balance between antimicrobial activity and cytotoxicity. |
| first_indexed | 2024-03-09T22:03:47Z |
| format | Article |
| id | doaj.art-a328b674f7d14d769d150485641d6bc9 |
| institution | Directory Open Access Journal |
| issn | 2076-3417 |
| language | English |
| last_indexed | 2024-03-09T22:03:47Z |
| publishDate | 2022-09-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Applied Sciences |
| spelling | doaj.art-a328b674f7d14d769d150485641d6bc92023-11-23T19:44:35ZengMDPI AGApplied Sciences2076-34172022-09-011219971510.3390/app12199715Cytotoxicity and Antimicrobial Activity of BioAkt<sup>TM</sup> and Phytic Acid: A Laboratory-Based StudyPaolo Bertoletti0Matteo Salvadori1Riccardo Tonini2Diletta Forgione3Jacopo Francinelli4Maria Luisa Garo5Stefano Salgarello6Department of Medical and Surgery Specialties, Radiological Sciences and Public Health, Dental School, University of Brescia, 25121 Brescia, ItalyDepartment of Medical and Surgery Specialties, Radiological Sciences and Public Health, Dental School, University of Brescia, 25121 Brescia, ItalyDepartment of Medical and Surgery Specialties, Radiological Sciences and Public Health, Dental School, University of Brescia, 25121 Brescia, ItalyDepartment of Medical and Surgery Specialties, Radiological Sciences and Public Health, Dental School, University of Brescia, 25121 Brescia, ItalyDepartment of Medical and Surgery Specialties, Radiological Sciences and Public Health, Dental School, University of Brescia, 25121 Brescia, ItalyDepartment of Medical and Surgery Specialties, Radiological Sciences and Public Health, Dental School, University of Brescia, 25121 Brescia, ItalyDepartment of Medical and Surgery Specialties, Radiological Sciences and Public Health, Dental School, University of Brescia, 25121 Brescia, Italy(1) Background: To improve endodontic treatments, it is necessary to find good irrigant solutions that balance potential toxic effects with optimal antimicrobial capacity. The aim of this laboratory study was to assess the cytotoxic and antimicrobial capacity of phytic acid (IP6) and BioAkt<sup>TM</sup> in a laboratory setting to determine their suitability prior to endodontic evaluation. (2) Methods: The antimicrobial and cytotoxic effects of IP6 and BioAkt<sup>TM</sup> were compared with those of 1.5% NaOCl. Cytotoxicity was evaluated via crystal violet assay and CellTiter-Glo<sup>®</sup> 2.0 assay, while antimicrobial capacity was tested via disk diffusion and the effect of a 1-h treatment on Enterococcus faecalis. (3) Results: A crystal violet test and CellTiter-Glo<sup>®</sup> 2.0 assay showed statistically significant differences in cell viability and cell activity after 4 and 24 h for all tested solutions (<i>p</i> < 0.05). The antimicrobial activity of BioAkt<sup>TM</sup> was similar to that of 1.5% NaOCl, while phytic acid showed the lowest antimicrobial activity compared to BioAkt<sup>TM</sup> (<i>p</i> < 0.05) and 1.5% NaOCl (<i>p</i> < 0.05). (4) Conclusion: BioAkt<sup>TM</sup> showed an optimal balance between antimicrobial activity and cytotoxicity.https://www.mdpi.com/2076-3417/12/19/9715BioAkt<sup>TM</sup>phytic acidcytotoxicityantimicrobial activitylaboratory study |
| spellingShingle | Paolo Bertoletti Matteo Salvadori Riccardo Tonini Diletta Forgione Jacopo Francinelli Maria Luisa Garo Stefano Salgarello Cytotoxicity and Antimicrobial Activity of BioAkt<sup>TM</sup> and Phytic Acid: A Laboratory-Based Study Applied Sciences BioAkt<sup>TM</sup> phytic acid cytotoxicity antimicrobial activity laboratory study |
| title | Cytotoxicity and Antimicrobial Activity of BioAkt<sup>TM</sup> and Phytic Acid: A Laboratory-Based Study |
| title_full | Cytotoxicity and Antimicrobial Activity of BioAkt<sup>TM</sup> and Phytic Acid: A Laboratory-Based Study |
| title_fullStr | Cytotoxicity and Antimicrobial Activity of BioAkt<sup>TM</sup> and Phytic Acid: A Laboratory-Based Study |
| title_full_unstemmed | Cytotoxicity and Antimicrobial Activity of BioAkt<sup>TM</sup> and Phytic Acid: A Laboratory-Based Study |
| title_short | Cytotoxicity and Antimicrobial Activity of BioAkt<sup>TM</sup> and Phytic Acid: A Laboratory-Based Study |
| title_sort | cytotoxicity and antimicrobial activity of bioakt sup tm sup and phytic acid a laboratory based study |
| topic | BioAkt<sup>TM</sup> phytic acid cytotoxicity antimicrobial activity laboratory study |
| url | https://www.mdpi.com/2076-3417/12/19/9715 |
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