Prevalence and Characterization of Extended-Spectrum β-Lactamase-Producing <i>Escherichia coli</i> Isolated from Dogs and Cats in South Korea
Overall, 836 <i>Escherichia coli</i> isolates (695 isolates from dogs and 141 from cats) were recovered from the diarrhea, skin/ear, urine, and genitals of dogs and cats between 2018 and 2019. Cefovecin and enrofloxacin resistance were noted in 17.1% and 21.2% of <i>E. coli</i&g...
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MDPI AG
2023-04-01
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| Series: | Antibiotics |
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| Online Access: | https://www.mdpi.com/2079-6382/12/4/745 |
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| author | Ji-Hyun Choi Md. Sekendar Ali Bo-Youn Moon Hee-Young Kang Su-Jeong Kim Hyun-Ju Song Abraham Fikru Mechesso Dong-Chan Moon Suk-Kyung Lim |
| author_facet | Ji-Hyun Choi Md. Sekendar Ali Bo-Youn Moon Hee-Young Kang Su-Jeong Kim Hyun-Ju Song Abraham Fikru Mechesso Dong-Chan Moon Suk-Kyung Lim |
| author_sort | Ji-Hyun Choi |
| collection | DOAJ |
| description | Overall, 836 <i>Escherichia coli</i> isolates (695 isolates from dogs and 141 from cats) were recovered from the diarrhea, skin/ear, urine, and genitals of dogs and cats between 2018 and 2019. Cefovecin and enrofloxacin resistance were noted in 17.1% and 21.2% of <i>E. coli</i> isolates, respectively. The cefovecin and enrofloxacin resistance rates were higher in dog isolates (18.1% and 22.9%) compared with the rates in cat isolates (12.1%, 12.8%). Interestingly, resistance to both antimicrobials was noted in 10.8% (90/836) of the isolates, predominantly in isolates from dogs. <i>bla</i><sub>CTX-M-14</sub>, <i>bla</i><sub>CTX-M-15</sub>, and <i>bla</i><sub>CMY-2</sub> were the most frequent extended-spectrum β-lactamase/plasmid-mediated AmpC β-lactamase (ESBL/AmpC)- gene types. The co-existence of <i>bla</i><sub>CTX-M and</sub><i>bla</i><sub>CMY-2</sub> was noted in six <i>E. coli</i> isolates from dogs. Sequencing analysis demonstrated that S83L and D87N in <i>gyrA</i> and S80I in <i>parC</i> were the most frequent point mutations in the quinolone resistance-determining regions of the cefovecin and enrofloxacin-resistant isolates. A total of 11 isolates from dogs carried the plasmid-mediated quinolone resistance genes (six <i>aac(6’)-Ib-cr</i>, four <i>qnrS</i>, and one <i>qnrB</i>), while only two cat isolates carried the <i>qnrS</i> gene. Multilocus sequence typing of the cefovecin and enrofloxacin-resistant isolates revealed that sequence type (ST)131 <i>E. coli</i> carrying <i>bla</i><sub>CTX-M-14</sub> and <i>bla</i><sub>CTX-M-15</sub> genes and ST405 <i>E. coli</i> carrying <i>bla</i><sub>CMY-2</sub> gene were predominant among the isolated <i>E. coli</i> strains. The majority of the ESBL/AmpC-producing isolates displayed diverse pulsed-field gel electrophoresis profiles. This study demonstrated that third-generation cephalosporin- and fluoroquinolone-resistant <i>E. coli</i> were widely distributed in companion animals. The detection of the pandemic ST131 clone carrying <i>bla</i><sub>CTX-M</sub>-<sub>14/15</sub> in companion animals presented a public health threat. |
| first_indexed | 2024-03-11T05:18:11Z |
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| institution | Directory Open Access Journal |
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| language | English |
| last_indexed | 2024-03-11T05:18:11Z |
| publishDate | 2023-04-01 |
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| series | Antibiotics |
| spelling | doaj.art-a32bb37cb29a473a9ea4e1a3d55d00872023-11-17T18:03:24ZengMDPI AGAntibiotics2079-63822023-04-0112474510.3390/antibiotics12040745Prevalence and Characterization of Extended-Spectrum β-Lactamase-Producing <i>Escherichia coli</i> Isolated from Dogs and Cats in South KoreaJi-Hyun Choi0Md. Sekendar Ali1Bo-Youn Moon2Hee-Young Kang3Su-Jeong Kim4Hyun-Ju Song5Abraham Fikru Mechesso6Dong-Chan Moon7Suk-Kyung Lim8Bacterial Disease Division, Animal and Plant Quarantine Agency, 177 Hyeksin 8-ro, Gimcheon-si 39660, Republic of KoreaBacterial Disease Division, Animal and Plant Quarantine Agency, 177 Hyeksin 8-ro, Gimcheon-si 39660, Republic of KoreaBacterial Disease Division, Animal and Plant Quarantine Agency, 177 Hyeksin 8-ro, Gimcheon-si 39660, Republic of KoreaBacterial Disease Division, Animal and Plant Quarantine Agency, 177 Hyeksin 8-ro, Gimcheon-si 39660, Republic of KoreaBacterial Disease Division, Animal and Plant Quarantine Agency, 177 Hyeksin 8-ro, Gimcheon-si 39660, Republic of KoreaBacterial Disease Division, Animal and Plant Quarantine Agency, 177 Hyeksin 8-ro, Gimcheon-si 39660, Republic of KoreaDepartment of Pathology and Microbiology, College of Medicine, University of Nebraska Medical Center, Omaha, NE 68198-5900, USADivision of Antimicrobial Resistance Research, Center for Infectious Diseases Research, Korea Disease Control and Prevention Agency, Cheongju 28159, Republic of KoreaBacterial Disease Division, Animal and Plant Quarantine Agency, 177 Hyeksin 8-ro, Gimcheon-si 39660, Republic of KoreaOverall, 836 <i>Escherichia coli</i> isolates (695 isolates from dogs and 141 from cats) were recovered from the diarrhea, skin/ear, urine, and genitals of dogs and cats between 2018 and 2019. Cefovecin and enrofloxacin resistance were noted in 17.1% and 21.2% of <i>E. coli</i> isolates, respectively. The cefovecin and enrofloxacin resistance rates were higher in dog isolates (18.1% and 22.9%) compared with the rates in cat isolates (12.1%, 12.8%). Interestingly, resistance to both antimicrobials was noted in 10.8% (90/836) of the isolates, predominantly in isolates from dogs. <i>bla</i><sub>CTX-M-14</sub>, <i>bla</i><sub>CTX-M-15</sub>, and <i>bla</i><sub>CMY-2</sub> were the most frequent extended-spectrum β-lactamase/plasmid-mediated AmpC β-lactamase (ESBL/AmpC)- gene types. The co-existence of <i>bla</i><sub>CTX-M and</sub><i>bla</i><sub>CMY-2</sub> was noted in six <i>E. coli</i> isolates from dogs. Sequencing analysis demonstrated that S83L and D87N in <i>gyrA</i> and S80I in <i>parC</i> were the most frequent point mutations in the quinolone resistance-determining regions of the cefovecin and enrofloxacin-resistant isolates. A total of 11 isolates from dogs carried the plasmid-mediated quinolone resistance genes (six <i>aac(6’)-Ib-cr</i>, four <i>qnrS</i>, and one <i>qnrB</i>), while only two cat isolates carried the <i>qnrS</i> gene. Multilocus sequence typing of the cefovecin and enrofloxacin-resistant isolates revealed that sequence type (ST)131 <i>E. coli</i> carrying <i>bla</i><sub>CTX-M-14</sub> and <i>bla</i><sub>CTX-M-15</sub> genes and ST405 <i>E. coli</i> carrying <i>bla</i><sub>CMY-2</sub> gene were predominant among the isolated <i>E. coli</i> strains. The majority of the ESBL/AmpC-producing isolates displayed diverse pulsed-field gel electrophoresis profiles. This study demonstrated that third-generation cephalosporin- and fluoroquinolone-resistant <i>E. coli</i> were widely distributed in companion animals. The detection of the pandemic ST131 clone carrying <i>bla</i><sub>CTX-M</sub>-<sub>14/15</sub> in companion animals presented a public health threat.https://www.mdpi.com/2079-6382/12/4/745β-lactamase<i>bla</i><sub>CTX-M</sub><i>bla</i><sub>CMY-2</sub>companion animals<i>E. coli</i>mutation |
| spellingShingle | Ji-Hyun Choi Md. Sekendar Ali Bo-Youn Moon Hee-Young Kang Su-Jeong Kim Hyun-Ju Song Abraham Fikru Mechesso Dong-Chan Moon Suk-Kyung Lim Prevalence and Characterization of Extended-Spectrum β-Lactamase-Producing <i>Escherichia coli</i> Isolated from Dogs and Cats in South Korea Antibiotics β-lactamase <i>bla</i><sub>CTX-M</sub> <i>bla</i><sub>CMY-2</sub> companion animals <i>E. coli</i> mutation |
| title | Prevalence and Characterization of Extended-Spectrum β-Lactamase-Producing <i>Escherichia coli</i> Isolated from Dogs and Cats in South Korea |
| title_full | Prevalence and Characterization of Extended-Spectrum β-Lactamase-Producing <i>Escherichia coli</i> Isolated from Dogs and Cats in South Korea |
| title_fullStr | Prevalence and Characterization of Extended-Spectrum β-Lactamase-Producing <i>Escherichia coli</i> Isolated from Dogs and Cats in South Korea |
| title_full_unstemmed | Prevalence and Characterization of Extended-Spectrum β-Lactamase-Producing <i>Escherichia coli</i> Isolated from Dogs and Cats in South Korea |
| title_short | Prevalence and Characterization of Extended-Spectrum β-Lactamase-Producing <i>Escherichia coli</i> Isolated from Dogs and Cats in South Korea |
| title_sort | prevalence and characterization of extended spectrum β lactamase producing i escherichia coli i isolated from dogs and cats in south korea |
| topic | β-lactamase <i>bla</i><sub>CTX-M</sub> <i>bla</i><sub>CMY-2</sub> companion animals <i>E. coli</i> mutation |
| url | https://www.mdpi.com/2079-6382/12/4/745 |
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