Development of Pretreatment Strategies for the Fractionation of Hazelnut Shells in the Scope of Biorefinery
Hazelnut shells are an important waste from the hazelnut processing industry that could be valorized in a multi-product biorefinery. Individual or combined pretreatments may be integrated in processes enabling the integral fractionation of biomass. In this study, fractionation methods based on alkal...
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| Format: | Article |
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MDPI AG
2020-10-01
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| Series: | Agronomy |
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| Online Access: | https://www.mdpi.com/2073-4395/10/10/1568 |
| _version_ | 1797551016337473536 |
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| author | Laura López Sandra Rivas Andrés Moure Carlos Vila Juan Carlos Parajó |
| author_facet | Laura López Sandra Rivas Andrés Moure Carlos Vila Juan Carlos Parajó |
| author_sort | Laura López |
| collection | DOAJ |
| description | Hazelnut shells are an important waste from the hazelnut processing industry that could be valorized in a multi-product biorefinery. Individual or combined pretreatments may be integrated in processes enabling the integral fractionation of biomass. In this study, fractionation methods based on alkaline, alkaline-organosolv, organosolv, or acid-catalyzed organosolv treatments were applied to raw or autohydrolyzed hazelnut shells. A comparative analysis of results confirmed that the highest lignin removal was achieved with the acid-catalyzed organosolv delignification, which also allowed limited cellulose losses. When this treatment was applied to raw hazelnut shells, 65.3% of the lignin was removed, valuable hemicellulose-derived products were obtained, and the cellulose content of the processed solids increased up to 54%. Autohydrolysis of hazelnut shells resulted in the partial solubilization of hemicelluloses (mainly in the form of soluble oligosaccharides). Consecutive stages of autohydrolysis and acid-catalyzed organosolv delignification resulted in 47.9% lignin removal, yielding solids of increased cellulose content (55.4%) and very low content of residual hemicelluloses. The suitability of selected delignified and autohydrolyzed-delignified hazelnut shells as substrates for enzymatic hydrolysis was assessed in additional experiments. The most susceptible substrates (from acid-catalyzed organosolv treatments) reached 74.2% cellulose conversion into glucose, with a concentration of 28.52 g glucose/L. |
| first_indexed | 2024-03-10T15:38:45Z |
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| id | doaj.art-a32c10d3252e4590ab3b6bcdd8c2e835 |
| institution | Directory Open Access Journal |
| issn | 2073-4395 |
| language | English |
| last_indexed | 2024-03-10T15:38:45Z |
| publishDate | 2020-10-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Agronomy |
| spelling | doaj.art-a32c10d3252e4590ab3b6bcdd8c2e8352023-11-20T17:04:18ZengMDPI AGAgronomy2073-43952020-10-011010156810.3390/agronomy10101568Development of Pretreatment Strategies for the Fractionation of Hazelnut Shells in the Scope of BiorefineryLaura López0Sandra Rivas1Andrés Moure2Carlos Vila3Juan Carlos Parajó4Department of Chemical Engineering, University of Vigo (Campus Ourense), 32004 As Lagoas, Ourense, SpainDepartment of Chemical Engineering, University of Vigo (Campus Ourense), 32004 As Lagoas, Ourense, SpainDepartment of Chemical Engineering, University of Vigo (Campus Ourense), 32004 As Lagoas, Ourense, SpainDepartment of Chemical Engineering, University of Vigo (Campus Ourense), 32004 As Lagoas, Ourense, SpainDepartment of Chemical Engineering, University of Vigo (Campus Ourense), 32004 As Lagoas, Ourense, SpainHazelnut shells are an important waste from the hazelnut processing industry that could be valorized in a multi-product biorefinery. Individual or combined pretreatments may be integrated in processes enabling the integral fractionation of biomass. In this study, fractionation methods based on alkaline, alkaline-organosolv, organosolv, or acid-catalyzed organosolv treatments were applied to raw or autohydrolyzed hazelnut shells. A comparative analysis of results confirmed that the highest lignin removal was achieved with the acid-catalyzed organosolv delignification, which also allowed limited cellulose losses. When this treatment was applied to raw hazelnut shells, 65.3% of the lignin was removed, valuable hemicellulose-derived products were obtained, and the cellulose content of the processed solids increased up to 54%. Autohydrolysis of hazelnut shells resulted in the partial solubilization of hemicelluloses (mainly in the form of soluble oligosaccharides). Consecutive stages of autohydrolysis and acid-catalyzed organosolv delignification resulted in 47.9% lignin removal, yielding solids of increased cellulose content (55.4%) and very low content of residual hemicelluloses. The suitability of selected delignified and autohydrolyzed-delignified hazelnut shells as substrates for enzymatic hydrolysis was assessed in additional experiments. The most susceptible substrates (from acid-catalyzed organosolv treatments) reached 74.2% cellulose conversion into glucose, with a concentration of 28.52 g glucose/L.https://www.mdpi.com/2073-4395/10/10/1568biorefineryhazelnut shellshydrothermal pretreatmentdelignificationenzymatic hydrolysisfractionation |
| spellingShingle | Laura López Sandra Rivas Andrés Moure Carlos Vila Juan Carlos Parajó Development of Pretreatment Strategies for the Fractionation of Hazelnut Shells in the Scope of Biorefinery Agronomy biorefinery hazelnut shells hydrothermal pretreatment delignification enzymatic hydrolysis fractionation |
| title | Development of Pretreatment Strategies for the Fractionation of Hazelnut Shells in the Scope of Biorefinery |
| title_full | Development of Pretreatment Strategies for the Fractionation of Hazelnut Shells in the Scope of Biorefinery |
| title_fullStr | Development of Pretreatment Strategies for the Fractionation of Hazelnut Shells in the Scope of Biorefinery |
| title_full_unstemmed | Development of Pretreatment Strategies for the Fractionation of Hazelnut Shells in the Scope of Biorefinery |
| title_short | Development of Pretreatment Strategies for the Fractionation of Hazelnut Shells in the Scope of Biorefinery |
| title_sort | development of pretreatment strategies for the fractionation of hazelnut shells in the scope of biorefinery |
| topic | biorefinery hazelnut shells hydrothermal pretreatment delignification enzymatic hydrolysis fractionation |
| url | https://www.mdpi.com/2073-4395/10/10/1568 |
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