Analysis of Ser/Thr Kinase HASPIN-Interacting Proteins in the Spermatids
HASPIN is predominantly expressed in spermatids, and plays an important role in cell division in somatic and meiotic cells through histone H3 phosphorylation. The literature published to date has suggested that HASPIN may play multiple roles in cells. Here, 10 gene products from the mouse testis cDN...
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| Format: | Article |
| Language: | English |
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MDPI AG
2022-08-01
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| Series: | International Journal of Molecular Sciences |
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| Online Access: | https://www.mdpi.com/1422-0067/23/16/9060 |
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| author | Naoko Maeda Junji Tsuchida Yoshitake Nishimune Hiromitsu Tanaka |
| author_facet | Naoko Maeda Junji Tsuchida Yoshitake Nishimune Hiromitsu Tanaka |
| author_sort | Naoko Maeda |
| collection | DOAJ |
| description | HASPIN is predominantly expressed in spermatids, and plays an important role in cell division in somatic and meiotic cells through histone H3 phosphorylation. The literature published to date has suggested that HASPIN may play multiple roles in cells. Here, 10 gene products from the mouse testis cDNA library that interact with HASPIN were isolated using the two-hybrid system. Among them, CENPJ/CPAP, KPNA6/importin alpha 6, and C1QBP/HABP1 were analyzed in detail for their interactions with HASPIN, with HASPIN phosphorylated C1QBP as the substrate. The results indicated that HASPIN is involved in spermatogenesis through the phosphorylation of C1QBP in spermatids, and also may be involved in the formation of centrosomes. |
| first_indexed | 2024-03-09T09:56:34Z |
| format | Article |
| id | doaj.art-a332e91f7e1b46d19533f2403aac4470 |
| institution | Directory Open Access Journal |
| issn | 1661-6596 1422-0067 |
| language | English |
| last_indexed | 2024-03-09T09:56:34Z |
| publishDate | 2022-08-01 |
| publisher | MDPI AG |
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| series | International Journal of Molecular Sciences |
| spelling | doaj.art-a332e91f7e1b46d19533f2403aac44702023-12-01T23:47:15ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672022-08-012316906010.3390/ijms23169060Analysis of Ser/Thr Kinase HASPIN-Interacting Proteins in the SpermatidsNaoko Maeda0Junji Tsuchida1Yoshitake Nishimune2Hiromitsu Tanaka3Department of Science for Laboratory Animal Experimentation, Research Institute for Microbial Diseases, Osaka University, 3-1 Yamadaoka, Suita 565-0871, Osaka, JapanDepartment of Science for Laboratory Animal Experimentation, Research Institute for Microbial Diseases, Osaka University, 3-1 Yamadaoka, Suita 565-0871, Osaka, JapanDepartment of Science for Laboratory Animal Experimentation, Research Institute for Microbial Diseases, Osaka University, 3-1 Yamadaoka, Suita 565-0871, Osaka, JapanFaculty of Pharmaceutical Sciences, Nagasaki International University, 2825-7 Huis Ten Bosch, Sasebo 859-3298, Nagasaki, JapanHASPIN is predominantly expressed in spermatids, and plays an important role in cell division in somatic and meiotic cells through histone H3 phosphorylation. The literature published to date has suggested that HASPIN may play multiple roles in cells. Here, 10 gene products from the mouse testis cDNA library that interact with HASPIN were isolated using the two-hybrid system. Among them, CENPJ/CPAP, KPNA6/importin alpha 6, and C1QBP/HABP1 were analyzed in detail for their interactions with HASPIN, with HASPIN phosphorylated C1QBP as the substrate. The results indicated that HASPIN is involved in spermatogenesis through the phosphorylation of C1QBP in spermatids, and also may be involved in the formation of centrosomes.https://www.mdpi.com/1422-0067/23/16/9060C1QBPCENPJKPNARANBP9MORNTcp10 |
| spellingShingle | Naoko Maeda Junji Tsuchida Yoshitake Nishimune Hiromitsu Tanaka Analysis of Ser/Thr Kinase HASPIN-Interacting Proteins in the Spermatids International Journal of Molecular Sciences C1QBP CENPJ KPNA RANBP9 MORN Tcp10 |
| title | Analysis of Ser/Thr Kinase HASPIN-Interacting Proteins in the Spermatids |
| title_full | Analysis of Ser/Thr Kinase HASPIN-Interacting Proteins in the Spermatids |
| title_fullStr | Analysis of Ser/Thr Kinase HASPIN-Interacting Proteins in the Spermatids |
| title_full_unstemmed | Analysis of Ser/Thr Kinase HASPIN-Interacting Proteins in the Spermatids |
| title_short | Analysis of Ser/Thr Kinase HASPIN-Interacting Proteins in the Spermatids |
| title_sort | analysis of ser thr kinase haspin interacting proteins in the spermatids |
| topic | C1QBP CENPJ KPNA RANBP9 MORN Tcp10 |
| url | https://www.mdpi.com/1422-0067/23/16/9060 |
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